Abstract:
:The results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. It has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transformations of substrates. A partial phase diagram for a system consisting of glucose, water, and (poly)ethylene glycol was constructed to identify the regions which are most suitable for biotransformations. It was confirmed that the glass transition in this system occurred below the reaction temperature at any given composition of the constituent components. Several glycosidases were found to be catalytically active in this medium and the activity of beta-glucosidase from almond was determined at several compositions of the reaction mixture and related to the corresponding regions of the phase diagram. The synthetic utility of the system was illustrated by glucosylation of several alpha,omega-alkyldiols, short-chain polyethylene glycols, and hydroxyalkyl and glyceryl monoacrylates.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Millqvist-Fureby A,Gill IS,Vulfson ENsubject
Has Abstractpub_date
1998-10-20 00:00:00pages
190-6issue
2eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19981020)60:2<190::AID-BITjournal_volume
60pub_type
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