Abstract:
:Pseudomonas putida cells were grown in confined volumes in dual-membrane immobilized cell reactors constructed from microporous polyethylene hollow fibers and silicone rubber tubules as a model system for the study of mass transport in microbial aggregates. Local cell concentrations in the reactors reached 300 g dry mass/L. Pulse-chase radioisotope labeling with (35)SO(4) (2-) was used to estimate the rates of cell mass synthesis and degradation. Sulfur incorporation consistently exceeded sulfur release, implying that the cell mass concentration continually increases. The location and size of the cell growth region was determined using liquid emulsion autoradiography of thin sections prepared from labeled reactors. Cell growth occurs in a region less than 25 mum in depth adjacent to the oxygen supply, and the expansion of the cells caused by cell growth promotes convection of the cell mass into regions of the reactor where starving cells accumulate. The combination of mass-balance and spatial distribution measurements that can be made using radioisotope tracers provides a versatile method for determining metabolic rates and limitations caused by mass transfer in immobilized cell reactors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Karel SF,Robertson CRdoi
10.1002/bit.260340307subject
Has Abstractpub_date
1989-07-01 00:00:00pages
320-36issue
3eissn
0006-3592issn
1097-0290journal_volume
34pub_type
杂志文章abstract::Cellulosomes are large plant cell wall degrading complexes secreted by some anaerobic bacteria. They are typically composed of a major scaffolding protein containing multiple receptors called cohesins, which tightly anchor a small complementary module termed dockerin harbored by the cellulosomal enzymes. In the presen...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27242
更新日期:2020-03-01 00:00:00
abstract::Equilibrium adsorption studies on Escherichia coli and Saccharomyces sp. revealed the capacity and affinity of these organisms for the surfaces of powdered charcoal and nickel. In simple salt solutions both organisms readily adsorbed to each solid with an affinity and maximum loading capacity individual to each cell-s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340703
更新日期:1989-10-05 00:00:00
abstract::An unadapted mixed methanogenic sludge transformed formaldehyde into methanol and formate. The methanol to formate ratio obtained was 1:1. Formaldehyde conversion proceeded without any lag phase, suggesting the constitutive character of the formaldehyde conversion enzymes involved. Because the rate of formaldehyde con...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10294
更新日期:2002-08-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360204
更新日期:1990-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260351002
更新日期:1990-04-25 00:00:00
abstract::Bioprocess identification starts with collection of process information. Usually there is a variety of information available, consisting of actual measurement data, historical data, empirical kinetic and yield correlations, and general knowledge available from literature. A central problem is to find out how the vario...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960220)49:4<364::AID-BIT
更新日期:1996-02-20 00:00:00
abstract::The feasibility of producing ethanol in a continuous system from cellulose using Clostridirrrn thermocellum was investigated. This anaerobic and therniophilic bacterium was able to degrade cellulose directly into ethanol with acetic acid, hydrogen. and carbon dioxide as by-products of this fermentation. The fermentati...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240106
更新日期:1982-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980320)57:6<704::aid-bit
更新日期:1998-03-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280107
更新日期:1986-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22753
更新日期:2010-08-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260361007
更新日期:1990-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26725
更新日期:2018-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960805)51:3<271::AID-BIT
更新日期:1996-08-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20146
更新日期:2004-05-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1999-09-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360609
更新日期:1990-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240810
更新日期:1982-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370808
更新日期:1991-04-05 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2009-03-01 00:00:00
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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更新日期:2006-09-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10913
更新日期:2004-03-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2006-07-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260460204
更新日期:1995-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:1998-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1997-12-05 00:00:00