Plasmid presence changes the relative levels of many host cell proteins and ribosome components in recombinant Escherichia coli.

Abstract:

:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor of plasmid replication. At the intermediate plasmid copy number of 56, levels of several of the TCA cycle enzymes (oxoglutarate dehydrogenase complex, succinate thiokinase, and succinate dehydrogenase) as well as in aspartate transcarbamoylase increased. At a plasmid copy number of 240, higher amounts of PEP carboxylase as well as several of the heat shock proteins were observed. Furthermore, at high plasmid levels, significant decreases occurred in growth rate, pyruvate kinase I, pyruvate dehydrogenase complex, unadenylated glutamine synthetase, aspartate transcarbamoylase as well as in several of the proteins involved in translation. Decreases in ribosome content as well as in the free 30S and 50S ribosomal subunit pool fractions were also observed in separate analyses. These results indicate that recombinant DNA manipulations can cause major alterations in numerous host cell properties which could significantly influence cloned protein production or metabolic engineering endeavors.

journal_name

Biotechnol Bioeng

authors

Birnbaum S,Bailey JE

doi

10.1002/bit.260370808

subject

Has Abstract

pub_date

1991-04-05 00:00:00

pages

736-45

issue

8

eissn

0006-3592

issn

1097-0290

journal_volume

37

pub_type

杂志文章
  • "Enzyme Test Bench," a high-throughput enzyme characterization technique including the long-term stability.

    abstract::A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.22242

    authors: Rachinskiy K,Schultze H,Boy M,Bornscheuer U,Büchs J

    更新日期:2009-06-01 00:00:00

  • Simulations of host-plasmid interactions in Escherichia coli: Copy number, promoter strength, and ribosome binding site strength effects on metabolic activity and plasmid gene expression.

    abstract::A mechanistically detailed single-cell model E. coli B/r-A was adapted to simulate the effects of vector presence on cell metabolism. Competition for RNA polymerase between chromosome and plasmid DNA is explicitly included. Distribution of active ribosomes among chromosome- and plasmid-derived messenger RNA, another k...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260290305

    authors: Peretti SW,Bailey JE

    更新日期:1987-02-01 00:00:00

  • Binding affinities/avidities of antibody-antigen interactions: quantification and scale-up implications.

    abstract::Bioaffinity interactions have been, and continue to be, successfully adapted from nature for use in separation and detection applications. It has been previously reported that the magnetophoretic mobility of labeled cells show a saturation type phenomenon as a function of the concentration of the free antibody-magneti...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21024

    authors: Zhang H,Williams PS,Zborowski M,Chalmers JJ

    更新日期:2006-12-05 00:00:00

  • Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

    abstract::Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (GFP) and a monoclonal antibody as reporter proteins. Following t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21972

    authors: Chenuet S,Martinet D,Besuchet-Schmutz N,Wicht M,Jaccard N,Bon AC,Derouazi M,Hacker DL,Beckmann JS,Wurm FM

    更新日期:2008-12-01 00:00:00

  • Affinity purification of plasmid DNA directly from crude bacterial cell lysates.

    abstract::We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21492

    authors: Darby RA,Forde GM,Slater NK,Hine AV

    更新日期:2007-12-01 00:00:00

  • Classification, error detection, and reconciliation of process information in complex biochemical systems.

    abstract::Bioprocess identification starts with collection of process information. Usually there is a variety of information available, consisting of actual measurement data, historical data, empirical kinetic and yield correlations, and general knowledge available from literature. A central problem is to find out how the vario...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19960220)49:4<364::AID-BIT

    authors: Noorman HJ,Romein B,Luyben KC,Heijnen JJ

    更新日期:1996-02-20 00:00:00

  • Impact of membrane solid-liquid separation on design of biological nutrient removal activated sludge systems.

    abstract::Installing membranes for solid-liquid separation into biological nutrient removal (BNR) activated sludge (AS) systems makes a profound difference not only in the design of the BNR system itself, but also in the design approach for the whole wastewater treatment plant (WWTP). In multizone BNR systems with membranes in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20311

    authors: Ramphao M,Wentzel MC,Merritt R,Ekama GA,Young T,Buckley CA

    更新日期:2005-03-20 00:00:00

  • Dynamic localization and persistent stimulation of factor-dependent cells by a stem cell factor / cellulose binding domain fusion protein.

    abstract::The extracellular matrix provides structural components that support the development of tissue morphology and the distribution of growth factors that modulate the overall cellular response to those growth factors. The ability to manipulate the presentation of factors in culture systems should provide an additional deg...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20611

    authors: Jervis EJ,Guarna MM,Doheny JG,Haynes CA,Kilburn DG

    更新日期:2005-08-05 00:00:00

  • Temperature and oxygen level determine N2 O respiration activities of heterotrophic N2 O-reducing bacteria: Biokinetic study.

    abstract::Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.27654

    authors: Zhou Y,Suenaga T,Qi C,Riya S,Hosomi M,Terada A

    更新日期:2020-12-11 00:00:00

  • Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multiparameter flow cytometry: effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration.

    abstract::Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

    更新日期:2000-11-20 00:00:00

  • Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet.

    abstract::Modification of cellular functions by overexpression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21738

    authors: Ino K,Kawasumi T,Ito A,Honda H

    更新日期:2008-05-01 00:00:00

  • A theoretical method to improve and optimize the design of bioartificial livers.

    abstract::Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.22765

    authors: Davidson AJ,Ellis MJ,Chaudhuri JB

    更新日期:2010-08-15 00:00:00

  • Automatic on-line fermentation headspace gas analysis using a computer-controlled gas chromatograph.

    abstract::A computer-controlled headspace gas chromatograph was used to monitor the progress of ethanol production from both aerobic batch and anaerobic continuous fermentations. Using an automatic, electropneumatic sampling system, aliquots of fermentation headspace gas were injected directly onto the column for quantitative e...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260251103

    authors: Comberbach DM,Bu'lock JD

    更新日期:1983-11-01 00:00:00

  • Dual substrate biodegradation of a nonionic surfactant and pentachlorophenol by Sphingomonas chlorophenolica RA2.

    abstract::The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20365

    authors: Bielefeldt AR,Cort T

    更新日期:2005-03-20 00:00:00

  • Selective adsorption of plant products.

    abstract::The results from this study demonstrate that neutral resins can selectively concentrate specific indole alkaloids from dilute aqueous mixtures. Adsorption was observed to provide a one to two order of magnitude improvement in concentrating these alkaloids, as compared to an equivalent single-staged extraction. Since t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260310905

    authors: Payne GF,Shuler ML

    更新日期:1988-06-05 00:00:00

  • Biotransformation of D-methionine into L-methionine in the cascade of four enzymes.

    abstract::D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21501

    authors: Findrik Z,Vasić-Racki D

    更新日期:2007-12-01 00:00:00

  • Optimization of rifamycin B fermentation in shake flasks via a machine-learning-based approach.

    abstract::Rifamycin B is an important polyketide antibiotic used in the treatment of tuberculosis and leprosy. We present results on medium optimization for Rifamycin B production via a barbital insensitive mutant strain of Amycolatopsis mediterranei S699. Machine-learning approaches such as Genetic algorithm (GA), Neighborhood...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20056

    authors: Bapat PM,Wangikar PP

    更新日期:2004-04-20 00:00:00

  • High-yield retroviral production using a temperature-modulated two-stage operation.

    abstract::For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20435

    authors: Kwon YJ,Peng CA

    更新日期:2005-05-05 00:00:00

  • Production of S-adenosyl-L-methionine by Saccharomyces cerevisiae cells carrying a gene for ethionine resistance.

    abstract::A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260351107

    authors: Shiomi N,Fukuda H,Fukuda Y,Murata K,Kimura A

    更新日期:1990-05-01 00:00:00

  • Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography.

    abstract::A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20738

    authors: Kempe H,Persson P,Axelsson A,Nilsson B,Zacchi G

    更新日期:2006-03-05 00:00:00

  • Evaluation of selectivity changes in HIC systems using a preferential interaction based analysis.

    abstract::It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20120

    authors: Xia F,Nagrath D,Garde S,Cramer SM

    更新日期:2004-08-05 00:00:00

  • Immobilization of Streptomyces phaerochromogenes by radiation-induced polymerization of glass-forming monomers.

    abstract::Immobilization of Streptomyces phaerochromogenes was studied by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures. Radiation damage of the enzyme could be avoided by choosing irradiation at low temperatures. The enzymatic activity of immobilized cells increased remarkably with a decre...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260210412

    authors: Kumakura M,Yoshida M,Kaetsu I

    更新日期:1979-04-01 00:00:00

  • Proposed mechanism of acetate accumulation in two recombinant Escherichia coli strains during high density fermentation.

    abstract::The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (lambdaDE3) and JM109, differ significantly in their acetate production during batch fermentation at high initial glucose con...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0290(19980105)57:1<71::aid-bit9

    authors: van de Walle M,Shiloach J

    更新日期:1998-01-05 00:00:00

  • Enzymatic reaction kinetic: comparison in an organic solvent and in supercritical carbon dioxide.

    abstract::Myristic acid esterification has been performed by an immobilized lipase from Mucor Miehei both in n-hexane and in supercritical carbon dioxide (SCCO(2)). The enzyme is stable in SCCO(2) at 15 MPa and 323 K. The reaction rate is influenced by the concentration of water and by the reaction medium composition. A reactio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260400218

    authors: Dumont T,Barth D,Corbier C,Branlant G,Perrut M

    更新日期:1992-06-20 00:00:00

  • Use of the abiotic proton balance for determining linear relations among net conversion rates of primary metabolites in fermentation processes.

    abstract::Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19961220)52:6<713::AID-BIT

    authors: Siano SA

    更新日期:1996-12-20 00:00:00

  • Maximum production strategy for biodegradable copolymer P(HB-co-HV) in fed-batch culture of Alcaligenes eutrophus.

    abstract::A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Shimizu H,Kozaki Y,Kodama H,Shioya S

    更新日期:1999-03-05 00:00:00

  • Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains.

    abstract::A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10428

    authors: Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

    更新日期:2002-12-30 00:00:00

  • Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Reprinted from Biotechnology and Bioengineering, Vol. XI, Issue 3, Pages 269-281 (1968).

    abstract::A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected...

    journal_title:Biotechnology and bioengineering

    pub_type: 传,古典文章,历史文章,杂志文章

    doi:10.1002/(sici)1097-0290(20000320)67:6<691::aid-bit

    authors: Cooney CL,Wang DI,Mateles RI

    更新日期:2000-03-20 00:00:00

  • Multiplexed gene transfer to a human T-cell line by combining Sleeping Beauty transposon system with methotrexate selection.

    abstract::Engineered human T-cells are a promising therapeutic modality for cancer immunotherapy. T-cells expressing chimeric antigen receptors combined with additional genes to enhance T-cell proliferation, survival, or tumor targeting may further improve efficacy but require multiple stable gene transfer events. Methods are t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.25538

    authors: Kacherovsky N,Liu GW,Jensen MC,Pun SH

    更新日期:2015-07-01 00:00:00

  • The kinetics of protein precipitation by different reagents.

    abstract::The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size wit...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260280312

    authors: Chan MY,Hoare M,Dunnill P

    更新日期:1986-03-01 00:00:00