Strain engineering for high-level 5-aminolevulinic acid production in Escherichia coli.

abstract：:The microbial degradation of quinoline by Comamonas acidovorans was studied in a laboratory scale stirred tank reactor. In continuous culture experiments using quinoline as a sole source of carbon and nitrogen, it was shown by means of mass balances that quinoline was converted completely to biomass, carbon dioxide, a...

journal_title：Biotechnology and bioengineering

authors： Miethling R,Hecht V,Deckwer WD

更新日期：1993-08-20 00:00:00

abstract：:An alpha-galactosidase and a beta-mannanase produced by the hyperthermophilic bacterium, Thermotoga neapolitana 5068 (TN5068), separately and together, were evaluated for their ability to hydrolyze guar gum in relation to viscosity reduction of guar-based hydraulic fracturing fluids used in oil and gas well stimulatio...

journal_title：Biotechnology and bioengineering

authors： McCutchen CM,Duffaud GD,Leduc P,Petersen AR,Tayal A,Khan SA,Kelly RM

更新日期：1996-10-20 00:00:00

abstract：:Downstream processing operations are often carried out blind in the process timescale since product monitoring on-line is not common. Knowledge of the location and concentration of the product and key contaminants is complementary to other process information for process development and, if available on-line in conjun...

journal_title：Biotechnology and bioengineering

authors： Holwill IJ,Chard SJ,Flanagan MT,Hoare M

更新日期：1997-01-05 00:00:00

abstract：:Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product...

journal_title：Biotechnology and bioengineering

authors： Somasundaram B,Pleitt K,Shave E,Baker K,Lua LHL

更新日期：2018-12-01 00:00:00

abstract：:Various esters were synthesized in nearly anhydrous hexane from alcohols and carboxylic acids using a lipase from Candida cylindracea. The enzyme was immobilized on a nylon support and protein loadings as high as 10 mg/g were obtained. The activity of the immobilized enzyme was maximum in a range of temperatures from ...

journal_title：Biotechnology and bioengineering

authors： Carta G,Gainer JL,Benton AH

更新日期：1991-05-01 00:00:00

abstract：:Biofouling is the undesirable adhesion and development of microorganisms and macroorganisms in a water environment. An electrochemical antifouling system based on management of primary adhesion of microorganisms was developed employing titanium electrode for antifouling of seawater cooling pipes and marine infrastruct...

journal_title：Biotechnology and bioengineering

authors： Wake H,Takahashi H,Takimoto T,Takayanagi H,Ozawa K,Kadoi H,Okochi M,Matsunaga T

更新日期：2006-10-20 00:00:00

abstract：:This study addresses the characterization of human islet-like structures generated from a newly discovered sparse population of precursor cells (Petropavlovskaia and Rosenberg, 2002) in the human pancreas. These cells may be progenitor cells capable of producing pancreatic cells suitable for the treatment of type 1 di...

journal_title：Biotechnology and bioengineering

authors： Bodnar CA,Sen A,Kallos MS,Behie LA,Petropavlovskaia M,Rosenberg L

更新日期：2006-04-05 00:00:00

abstract：:A bioremediation process to remove Co(2+) from aqueous solution is investigated in this study using a magnetic resonance imaging (MRI) protocol to rapidly obtain multiple 2D spatially resolved Co(2+) ion concentration maps. The MRI technique is described in detail and its ability to determine the evolution in both axi...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Holland DJ,Paterson-Beedle M,Macaskie LE,Gladden LF,Johns ML

更新日期：2008-03-01 00:00:00

abstract：:Cellulolytic fungi, such as Trichoderma reesei, T. lignorum, and Chaetomium cellulolyticum reach a low packing density of mycelia when grown on straw under conditions of solid substrate fermentation. The low packing density is shown to be caused partially by the geometric limitation of the growth of mycelia in the sub...

journal_title：Biotechnology and bioengineering

authors： Laukevics JJ,Apsite AF,Viesturs US,Tengerdy RP

更新日期：1985-12-01 00:00:00

abstract：:A denitrifying consortium capable of transforming carbon tetrachloride (CCl(4)) was cultured from aquifer sediment from the U.S. Department of Energy's Hanford Site in southeastern Washington State. To understand the kinetics of the biological destruction of CCl(4) by these microbes, a set of experiments, the conditio...

journal_title：Biotechnology and bioengineering

authors： Petersen JN,Skeen RS,Amos KM,Hooker BS

更新日期：1994-03-15 00:00:00

abstract：:Microbial degradation of trichloroethylene (TCE) has been demonstrated under aerobic conditions with propane. The primary objective of this research was to evaluate the feasibility of introducing a vapor phase form of TCE in the presence of propane to batch bioreactors containing a liquid phase suspension of Mycobacte...

journal_title：Biotechnology and bioengineering

authors： Wilcox DW,Autenrieth RL,Bonner JS

更新日期：1995-05-20 00:00:00

abstract：:Coastal bermudagrass was pretreated by a low-temperature ammonia fiber explosion (AFEX) process, which soaked the grass in liquid ammonia and then explosively released the pressure. Saccharifying enzymes were systematically applied to the AFEX-treated grass corresponding to low, medium, and high loadings of cellulase/...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Ripley EP,Nikolaou M

更新日期：1994-11-05 00:00:00

abstract：:The copolymerization method of immobilization was used to obtain preparations of enzymes covalently incorporated in polyacrylamide gel. They possess properties making them suitable for practical use. First, the preparations are hundreds of times more stable against irreversible thermoinactivation than native enzymes. ...

journal_title：Biotechnology and bioengineering

authors： Mozhaev VV,Siksnis VA,Torchilin VP,Martinek K

更新日期：1983-08-01 00:00:00

abstract：:The main processes involved in enhanced biological phosphorus removal (EBPR) under anaerobic and subsequently aerobic conditions are widely described in the literature. Polyphosphate accumulating organisms (PAO) are the organisms responsible for this process. However, the mechanisms of PAO are not fully established ye...

journal_title：Biotechnology and bioengineering

authors： Guisasola A,Pijuan M,Baeza JA,Carrera J,Casas C,Lafuente J

更新日期：2004-03-30 00:00:00

abstract：:The G-H loop of foot-and-mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine-glycine-aspartic acid tripeptide, a recognized integrin-binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide repr...

journal_title：Biotechnology and bioengineering

authors： Villaverde A,Feliu JX,Arís A,Harbottle RP,Benito A,Coutelle C

更新日期：1998-08-05 00:00:00

abstract：:The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have established a high resol...

journal_title：Biotechnology and bioengineering

authors： Amrhein S,Bauer KC,Galm L,Hubbuch J

更新日期：2015-12-01 00:00:00

abstract：:Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...

journal_title：Biotechnology and bioengineering

authors： Veleva AN,Cooper SL,Patterson C

更新日期：2007-09-01 00:00:00

abstract：:In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kin...

journal_title：Biotechnology and bioengineering

authors： Reuss M,Buchholz K

更新日期：1979-11-01 00:00:00

abstract：:The growth dynamics of a methanol utilizing bacterium, L3, in batch bioreactors were experimentally investigated. Formaldehyde, a key intermediate of methanol oxidation, is indicated to have a significant role in the complex batch growth behavior of L3. The intricate batch growth dynamics of many microorganisms can be...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Lim HC

更新日期：1984-11-01 00:00:00

abstract：:As nitrogen discharge limits are becoming more stringent, short-cut nitrogen systems and tertiary nitrogen polishing steps are gaining popularity. For partial nitritation or nitritation-denitritation systems, anaerobic ammonia oxidation (anammox) polishing may be feasible to remove residual ammonia and nitrite from th...

journal_title：Biotechnology and bioengineering

authors： Regmi P,Holgate B,Miller MW,Park H,Chandran K,Wett B,Murthy S,Bott CB

更新日期：2016-03-01 00:00:00

abstract：:The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhong JJ

更新日期：2005-02-20 00:00:00

abstract：:Molecular chaperones in aqueous-organic mixtures can broaden the utility of biocatalysis by stabilizing enzymes in denaturing conditions. We have designed a self-renaturing enzyme-chaperone chimera consisting of penicillin amidase and a thermophilic chaperonin that functions in aqueous-organic mixtures. The flexible l...

journal_title：Biotechnology and bioengineering

authors： Bergeron LM,Gomez L,Whitehead TA,Clark DS

更新日期：2009-04-01 00:00:00

abstract：:A cybernetic framework is presented which views microbial response in multiple substrate environments as a judicious investment of cellular resources in synthesizing different key proteins according to an optimal regulatory strategy. A mathematical model is developed within the cybernetic framework for the diauxic gro...

journal_title：Biotechnology and bioengineering

authors： Dhurjati P,Ramkrishna D,Flickinger MC,Tsao GT

更新日期：1985-01-01 00:00:00

abstract：:Membrane morphology is compared to protein depostion under passive adsorption and ultrafiltration conditions. Solute resistance of protein deposits for membranes of varying roughness, structure, and permeability can vary dramatically with operating conditions. Using Brunauer-Emmett-Teller adsorption isotherm (BET), st...

journal_title：Biotechnology and bioengineering

authors： Chen V,Kim KJ,Fane AG

更新日期：1995-07-20 00:00:00

abstract：:Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limi...

journal_title：Biotechnology and bioengineering

authors： Schoborg JA,Hershewe JM,Stark JC,Kightlinger W,Kath JE,Jaroentomeechai T,Natarajan A,DeLisa MP,Jewett MC

更新日期：2018-03-01 00:00:00

abstract：:Membrane adsorbers may be a viable alternative to the packed-bed chromatography for clearance of virus, host cell proteins, DNA, and other trace impurities. However, incorporation of membrane adsorbers into manufacturing processes has been slow due to the significant cost associated with obtaining regulatory approval ...

journal_title：Biotechnology and bioengineering

authors： Weaver J,Husson SM,Murphy L,Wickramasinghe SR

更新日期：2013-02-01 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00

abstract：:The hydrolysis of lactose by a beta-galactosidase from the thermophilic microorganism Thermus sp. strain T2, both in solution and immobilized on a commercial silica-alumina, has been studied. The enzyme has been previously produced by Escherichia coli JM101 harboring the plasmid pBGT1, which contains the codifying gen...

journal_title：Biotechnology and bioengineering

authors： Ladero M,Perez MT,Santos A,Garcia-Ochoa F

更新日期：2003-01-20 00:00:00

abstract：:A major challenge when designing cell scaffolds for chondrocyte delivery in vivo is creating scaffolds with sufficient mechanical properties to restore initial function while simultaneously controlling temporal changes in the gel structure to facilitate tissue formation. To address this design challenge, degradable ph...

journal_title：Biotechnology and bioengineering

authors： Bryant SJ,Bender RJ,Durand KL,Anseth KS

更新日期：2004-06-30 00:00:00

abstract：:A soil with aged contamination from lubricating oil (LO) and polychlorinated biphenyls (PCBs) was treated in a bioslurry reactor to investigate in-soil biosurfactant production by Pseudomonas aeruginosa, the most abundant indigenous, culturable, hydrocarbon-degrading microorganism. After 2 days of growth on LO, a depl...

journal_title：Biotechnology and bioengineering

authors： Hudak AJ,Cassidy DP

更新日期：2004-12-30 00:00:00