Encapsulating chondrocytes in degrading PEG hydrogels with high modulus: engineering gel structural changes to facilitate cartilaginous tissue production.

abstract：:Previous studies suggest that secretion of cloned proteins synthesized by recombinant Chinese hamster ovary (CHO) cells can be adenosine triphosphate (ATP) limited. Other research indicates that the presence of cloned Vitreoscilla hemoglobin (VHb) enhances ATP production in oxygen-limited Escherichia coli. To evaluate...

journal_title：Biotechnology and bioengineering

authors： Pendse GJ,Bailey JE

更新日期：1994-12-01 00:00:00

abstract：:Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of rando...

journal_title：Biotechnology and bioengineering

authors： McLachlan MJ,Johannes TW,Zhao H

更新日期：2008-02-01 00:00:00

abstract：:The morphology of mycelial fungi in liquid culture effects culture rheology and this in turn may affect product yield. It is therefore important to understand how environmental factors influence mycelial morphology and this paper describes the effect of dilution rate on two strains of Fusarium graminearum, the relativ...

journal_title：Biotechnology and bioengineering

authors： Wiebe MG,Trinci AP

更新日期：1991-06-05 00:00:00

abstract：:Plant cell walls are composed primarily of cellulose, hemicelluloses, lignins, and pectins. Of these components, lignins exhibit unique chemistry and physiological functions. Although lignins can be used as a product feedstock or as a fuel, lignins are also generally seen as a barrier to efficient enzymatic breakdown ...

journal_title：Biotechnology and bioengineering

authors： Donohoe BS,Decker SR,Tucker MP,Himmel ME,Vinzant TB

更新日期：2008-12-01 00:00:00

abstract：:Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

journal_title：Biotechnology and bioengineering

authors： Anish R,Rahman MS,Rao M

更新日期：2007-01-01 00:00:00

abstract：:Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...

journal_title：Biotechnology and bioengineering

authors： Satianegara G,Rogers PL,Rosche B

更新日期：2006-08-20 00:00:00

abstract：:Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

journal_title：Biotechnology and bioengineering

authors： Murphy A,O Fágáin C

更新日期：1998-05-20 00:00:00

abstract：:High throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput ap...

journal_title：Biotechnology and bioengineering

authors： Li H,Gao X,Demartini JD,Kumar R,Wyman CE

更新日期：2013-11-01 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTO...

journal_title：Biotechnology and bioengineering

authors： Kim MH,Wang N,McDonald T,Chu KH

更新日期：2012-12-01 00:00:00

abstract：:Ultrafiltration through Carbosep M(4) mineral membrane of protein solutions of decreasing complexity (whey before and after centrifugation or clarification, beta-lactoglobulin) was studied. Mathematical models were used to explain variations in flux with time. Taking into account variations in protein retention and hy...

journal_title：Biotechnology and bioengineering

authors： Taddei C,Daufin G,Aimar P,Sanchez V

更新日期：1989-06-20 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:The use of microalgae for biofuel production will be beneficial to society if we can produce biofuels at large scales with minimal mechanical energy input in the production process. Understanding micro-algal physiological responses under variable environmental conditions in bioreactors is essential for the optimizatio...

journal_title：Biotechnology and bioengineering

authors： Hansen TJ,Hondzo M,Mashek MT,Mashek DG,Lefebvre PA

更新日期：2013-01-01 00:00:00

abstract：:A refolding strategy was described for on-column refolding of recombinant human interferon-gamma (rhIFN-gamma) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-gamma protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded bed ...

journal_title：Biotechnology and bioengineering

authors： Jin T,Guan YX,Yao SJ,Lin DQ,Cho MG

更新日期：2006-03-05 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:The calculation of many derived fermentation variables such as the respiratory quotient (RQ) and mass transfer coefficient (K(L)a) requires the differences between the molar percentages of oxygen and carbon dioxide in the fermentor inlet and exit gas, called the %OUR and %CER. Noise and bias in %CER data is of order t...

journal_title：Biotechnology and bioengineering

authors： Royce PN,Thornhill NF

更新日期：1992-08-01 00:00:00

abstract：:In this article we present magnetic resonance microscopy (MRM) characterization of the advective transport in a biofilm capillary reactor. The biofilm generates non-axial flows that are up to 20% of the maximum axial velocity. The presence of secondary velocities of this magnitude alters the mass transport in the bior...

journal_title：Biotechnology and bioengineering

authors： Gjersing EL,Codd SL,Seymour JD,Stewart PS

更新日期：2005-03-30 00:00:00

abstract：:A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifyi...

journal_title：Biotechnology and bioengineering

authors： Terada A,Yamamoto T,Tsuneda S,Hirata A

更新日期：2006-07-05 00:00:00

abstract：:The microbial degradation of quinoline by Comamonas acidovorans was studied in a laboratory scale stirred tank reactor. In continuous culture experiments using quinoline as a sole source of carbon and nitrogen, it was shown by means of mass balances that quinoline was converted completely to biomass, carbon dioxide, a...

journal_title：Biotechnology and bioengineering

authors： Miethling R,Hecht V,Deckwer WD

更新日期：1993-08-20 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:The specific monoclonal antibody productivity (q(Mab)) of a murine hybridoma (CC9C10) increased with incubation temperature in the range 33 degrees C to 39 degrees C. q(Mab) was constant at each temperature and was independent of the phase of culture. The q(Mab) increased 97% at 39 degrees C and decreased by 21% at 33...

journal_title：Biotechnology and bioengineering

authors： Barnabé N,Butler M

更新日期：1994-11-20 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it is primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle for the development of recombinant strategies for IA production. Here, we created a library of...

journal_title：Biotechnology and bioengineering

authors： Jeon HG,Cheong DE,Han Y,Song JJ,Choi JH

更新日期：2016-07-01 00:00:00

abstract：:Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...

journal_title：Biotechnology and bioengineering

authors： Gaillet B,Gilbert R,Broussau S,Pilotte A,Malenfant F,Mullick A,Garnier A,Massie B

更新日期：2010-06-01 00:00:00

abstract：:Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of gen...

journal_title：Biotechnology and bioengineering

authors： Tee TW,Chowdhury A,Maranas CD,Shanks JV

更新日期：2014-05-01 00:00:00

abstract：:An analysis of the radial flow cell is presented to show that the assumption of creeping laminar flow should be used cautiously. Simple models which account for the influence of fluid inertial forces over most of the width of the plate are reviewed. A modified Reynolds number is introduced which may be used to test th...

journal_title：Biotechnology and bioengineering

authors： Fryer PJ,Slater NK,Duddridge JE

更新日期：1985-04-01 00:00:00

abstract：:The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...

journal_title：Biotechnology and bioengineering

authors： Ghose TK,Sahai V

更新日期：1979-02-01 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:The photosynthetic performance of a helical tubular photobioreactor ("Biocoil"), incorporating the filamentous cyanobacterium Spirulina platensis, was investigated. The photobioreactor was constructed in a cylindrical shape (0.9 m high) with a 0.25-m(2)basal area and a photostage comprising 60 m of transparent PVC tub...

journal_title：Biotechnology and bioengineering

authors： Watanabe Y,de la Noüe J,Hall DO

更新日期：1995-07-20 00:00:00