Abstract:
:Previous studies suggest that secretion of cloned proteins synthesized by recombinant Chinese hamster ovary (CHO) cells can be adenosine triphosphate (ATP) limited. Other research indicates that the presence of cloned Vitreoscilla hemoglobin (VHb) enhances ATP production in oxygen-limited Escherichia coli. To evaluate the influence of VHb expression on recombinant CHO cell productivity, the vhb gene has been fused to the mouse mammary tumor virus (MMTV) promoter and cloned in a CHO cell line previously engineered to express human tissue plasminogen activator (tPA). Western blot analysis confirms dexamethasone-inducible VHb expression in all of the clones tested. Batch cultivation experiments with one VHb-expressing clone and the parental CHO-tPA expressing cells. The VHb-expressing clone exhibits specific tPA production 40 to 100% greater than the parental CHO-tPA culture.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Pendse GJ,Bailey JEdoi
10.1002/bit.260441114subject
Has Abstractpub_date
1994-12-01 00:00:00pages
1367-70issue
11eissn
0006-3592issn
1097-0290journal_volume
44pub_type
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