The half-saturation coefficient for dissolved oxygen: A dynamic method for its determination and its effect on dual species competition.

abstract：:At the most fundamental level, saccharification occurs when cell wall degrading enzymes (CWDEs) diffuse, bind to and react on readily accessible cellulose fibrils. Thus, the study of the diffusive behavior of solutes into and out of cellulosic substrates is important for understanding how biomass pore size distributio...

journal_title：Biotechnology and bioengineering

authors： Yang D,Moran-Mirabal JM,Parlange JY,Walker LP

更新日期：2013-11-01 00:00:00

abstract：:This study examines protein adsorption behavior and the effects of mobile phase modifiers in multimodal chromatographic systems. Chromatography results with a diverse protein library indicate that multimodal and ion exchange resins have markedly different protein binding behavior and selectivity. NMR results corrobora...

journal_title：Biotechnology and bioengineering

authors： Holstein MA,Parimal S,McCallum SA,Cramer SM

更新日期：2012-01-01 00:00:00

abstract：:Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...

journal_title：Biotechnology and bioengineering

authors： Criddle CS

更新日期：1993-05-01 00:00:00

abstract：:Bioluminescence from the lux-based bacterial reporter Pseudomonas fluorescens HK44 was experimentally investigated under growth substrate-rich and limiting conditions in batch, continuous stirred tank (CSTR), and turbidostat reactors. A mechanistically based, mathematical model was developed to describe bioluminescenc...

journal_title：Biotechnology and bioengineering

authors： Kelly CJ,Hsiung CJ,Lajoie CA

更新日期：2003-02-05 00:00:00

abstract：:To study the influence of ammonium on an antibiotic cultivation, mass transfer measurements of ammonium through microporous hydrophobic membranes using different stripping methods were carried out and compared. The higher overall mass transfer coefficients for ammonium were obtained with an acid stripping solution com...

journal_title：Biotechnology and bioengineering

authors： Hecht V,Bischoff L,Gerth K

更新日期：1990-04-25 00:00:00

abstract：:A new biomass productivity model was parameterized for Chlorella vulgaris using short-term (<30 min) oxygen productivities from algal microcosms exposed to 6 light intensities (20-420 W/m(2)) and 6 temperatures (5-42 °C). The model was then validated against experimental biomass productivities recorded in bench-scale ...

journal_title：Biotechnology and bioengineering

authors： Béchet Q,Chambonnière P,Shilton A,Guizard G,Guieysse B

更新日期：2015-05-01 00:00:00

abstract：:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...

journal_title：Biotechnology and bioengineering

authors： Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA

更新日期：2015-09-01 00:00:00

abstract：:This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K(s) and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm r...

journal_title：Biotechnology and bioengineering

authors： Rittmann BE,Crawford L,Tuck CK,Namkung E

更新日期：1986-11-01 00:00:00

abstract：:The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([omim][PF(6)]), and in combination with supercr...

journal_title：Biotechnology and bioengineering

authors： Laszlo JA,Compton DL

更新日期：2001-10-20 00:00:00

abstract：:Many sites of environmental concern contain groundwater contaminated with nonaqueous phase liquids (NAPL). In such sites interfacial processes may affect both the equilibrium and kinetic behavior of the system. In particular, insoluble hydrocarbon partitioning and microbial biodegradation of insoluble hydrocarbon are ...

journal_title：Biotechnology and bioengineering

authors： Sekelsky AM,Shreve GS

更新日期：1999-05-20 00:00:00

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

journal_title：Biotechnology and bioengineering

authors： Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

更新日期：2008-08-01 00:00:00

abstract：:An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured...

journal_title：Biotechnology and bioengineering

authors： Xu M,Banc A,Woodruff TK,Shea LD

更新日期：2009-06-01 00:00:00

abstract：:We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

journal_title：Biotechnology and bioengineering

authors： Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

更新日期：2017-08-01 00:00:00

abstract：:This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

journal_title：Biotechnology and bioengineering

authors： Dos Santos LM,Livingston AG

更新日期：1995-07-05 00:00:00

abstract：:Using our recently developed sensor reactor approach, lysine-producing, nongrowing Corynebacterium glutamicum MH20-22B cells were subjected to serial (13)C-labeling experiments for flux analysis during the leucine-limited fed-batch production phase in a 300-L bioreactor. Based on two-dimensional (2D) nuclear magnetic ...

journal_title：Biotechnology and bioengineering

authors： Drysch A,El Massaoudi M,Wiechert W,de Graaf AA,Takors R

更新日期：2004-03-05 00:00:00

abstract：:The specific monoclonal antibody productivity (q(Mab)) of a murine hybridoma (CC9C10) increased with incubation temperature in the range 33 degrees C to 39 degrees C. q(Mab) was constant at each temperature and was independent of the phase of culture. The q(Mab) increased 97% at 39 degrees C and decreased by 21% at 33...

journal_title：Biotechnology and bioengineering

authors： Barnabé N,Butler M

更新日期：1994-11-20 00:00:00

abstract：:Peroxidase-catalyzed asymmetric sulfoxidations, while synthetically attractive, suffer from relatively low reaction rates due to poor substrate solubilities in water and from appreciable spontaneous oxidation of substrates (especially aryl alkyl sulfides) with H(2)O(2). In this work, we found that both of these shortc...

journal_title：Biotechnology and bioengineering

authors： Dai L,Klibanov AM

更新日期：2000-11-05 00:00:00

abstract：:Pre-filtration using ion exchange membrane adsorbers can improve parvovirus filter throughput of monoclonal antibodies (mAbs). The membranes work by binding trace foulants, and although some antibody product also binds, yields > or =99% are easily achieved by overloading. Results show that foulant adsorption is depend...

journal_title：Biotechnology and bioengineering

authors： Brown A,Bechtel C,Bill J,Liu H,Liu J,McDonald D,Pai S,Radhamohan A,Renslow R,Thayer B,Yohe S,Dowd C

更新日期：2010-07-01 00:00:00

abstract：:We developed a kinetic model that describes a heterogeneous reaction system for the production of D-p-hydroxyphenylglycine from D,L-p-hydroxyphenyl-hydantoin using D-hydantoinase of Bacillus stearothermophilus SD1 and N-carbamoylase of Agrobacterium tumefaciens NRRL B11291. As a biocatalyst, whole cells with separatel...

journal_title：Biotechnology and bioengineering

authors： Park JH,Oh KH,Lee DC,Kim HS

更新日期：2002-06-30 00:00:00

abstract：:Chemotaxis has been shown to be beneficial for the migration of soil-inhabiting bacteria towards industrial chemical pollutants, which they degrade. Many studies have demonstrated the importance of this microbial property under various circumstances; however, few quantitative analyses have been undertaken to measure t...

journal_title：Biotechnology and bioengineering

authors： Wang X,Atencia J,Ford RM

更新日期：2015-05-01 00:00:00

abstract：:For the application of immobilized enzymes, fixed bed reactors are used almost exclusively. Fixed bed reactors have specific disadvantages, especially for processes with a deactivating catalyst. Therefore, we have studied a novel reactor type with continuous transport of the immobilized biocatalyst. Flow of biocatalys...

journal_title：Biotechnology and bioengineering

authors： Vos HJ,Groen DJ,Potters JJ,Luyben KC

更新日期：1990-08-05 00:00:00

abstract：:Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...

journal_title：Biotechnology and bioengineering

authors： Jong JZ,Hsiun DY,Wu WT,Tzeng YM

更新日期：1995-11-05 00:00:00

abstract：:The heterogeneous nature of hollow fiber reactors for cell cultivation requires special considerations for proper design and operation. Downstream concentration of high-molecular-weight proteins has been measured in the shell side of ultrafiltration hollow fiber bioreactors. This distribution resulted from shell-side ...

journal_title：Biotechnology and bioengineering

authors： Piret JM,Cooney CL

更新日期：1990-11-01 00:00:00

abstract：:PHA, a naturally occurring biopolymer produced by a wide range of microorganisms, is known for its applications as bioplastic. In recent years the use of agro-industrial wastewater as substrate for PHA production by bacterial enrichments has attracted considerable research attention. Crude glycerol as generated during...

journal_title：Biotechnology and bioengineering

authors： Moralejo-Gárate H,Palmeiro-Sánchez T,Kleerebezem R,Mosquera-Corral A,Campos JL,van Loosdrecht MC

更新日期：2013-12-01 00:00:00

abstract：:Modification of cellular functions by overexpression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA...

journal_title：Biotechnology and bioengineering

authors： Ino K,Kawasumi T,Ito A,Honda H

更新日期：2008-05-01 00:00:00

abstract：:Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol-induced AOX1 promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes glycerol and biomass is accumulated; in th...

journal_title：Biotechnology and bioengineering

authors： Trinh LB,Phue JN,Shiloach J

更新日期：2003-05-20 00:00:00

abstract：:A kinetic model for the agglomeration of milk micelles following kappa-casein hydrolysis is described. The key features of the model are: (1) the surface potential of casein micelles is sufficient to explain the colloidal stability of the milk system; (2) the reduction in surface potential following kappa-casein hydro...

journal_title：Biotechnology and bioengineering

authors： Carlson A,Hill CG Jr,Olson NF

更新日期：1987-04-01 00:00:00

abstract：:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

journal_title：Biotechnology and bioengineering

authors： Flesher AR,Marzowski J,Wang WC,Raff HV

更新日期：1995-06-05 00:00:00

abstract：:Pretreatment methods were compared with steam explosion, and differing views on the relative importance of mechanical and chemical effects were outlined. Hydrolysis was desirable; pyrolysis was undesirable. The effects of initial moisture content on steam consumption, mechanism and rate of heat transfer, pentosan solu...

journal_title：Biotechnology and bioengineering

authors： Brownell HH,Saddler JN

更新日期：1987-02-01 00:00:00