Hollow fiber supported gas membrane for in situ removal of ammonium during an antibiotic fermentation.

abstract：:An automated system with semi on-line monitoring of glucose, lactic acid, protein, and optical density during lactic acid fermentations, is set up to study the dynamics of lactic acid bacteria. The analyzers for glucose, lactic acid, and protein are based on flow injection analysis (FIA). The system consists of a labo...

journal_title：Biotechnology and bioengineering

authors： Nielsen J,Nikolajsen K,Villadsen J

更新日期：1989-04-05 00:00:00

abstract：:The major objectives for preparative protein chromatography are maximal loading and increased flow rate while maintaining defined resolution. Conventionally a series of chromatographic experiments are performed and the optimal conditions are selected according to the separation criteria. Computer-aided process design ...

journal_title：Biotechnology and bioengineering

authors： Jungbauer A,Kaltenbrunner O

更新日期：1996-10-20 00:00:00

abstract：:Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase ...

journal_title：Biotechnology and bioengineering

authors： Kosugi Y,Tanaka H,Tomizuka N

更新日期：1990-09-01 00:00:00

abstract：:The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Araki K,Shioya S,Suga K

更新日期：1991-06-20 00:00:00

abstract：:Chromatography is the method of choice for the separation of proteins, at both analytical and preparative scale. Orthogonal purification strategies for industrial use can easily be implemented by combining different modes of adsorption. Nevertheless, with flexibility comes the freedom of choice and optimal conditions ...

journal_title：Biotechnology and bioengineering

authors： Dismer F,Hansen S,Oelmeier SA,Hubbuch J

更新日期：2013-03-01 00:00:00

abstract：:The hypothesis that frequency and amplitude response can be used in a complicated metabolic pathway kinetics model for optimal parameter estimation, as speculated by its successful prior usage for a mechanical oscillator and a heterogeneous chemical system, is tested here. Given the complexity of the glycolysis model ...

journal_title：Biotechnology and bioengineering

authors： Zimmerman WB

更新日期：2005-10-05 00:00:00

abstract：:The catalytic properties of bovine serum albumin (BSA) have been studied rather extensively. The protein has shown catalytic activities toward a variety of reactions in aqueous media. However, there appears to be no reports of reactions catalyzed exclusively in organic media. In this study, we report the catalytic pro...

journal_title：Biotechnology and bioengineering

authors： Liu XC,Lemma-Gray P

更新日期：2001-09-20 00:00:00

abstract：:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

journal_title：Biotechnology and bioengineering

authors： Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

更新日期：1997-06-05 00:00:00

abstract：:Antimicrobial peptides (AMPs) kill microbes by non-specific membrane permeabilization, making them ideal templates for designing novel peptide-based antibiotics that can combat multi-drug resistant pathogens. For maximum efficacy in vivo and in vitro, AMPs must be biocompatible, salt-tolerant and possess broad-spectru...

journal_title：Biotechnology and bioengineering

authors： Saravanan R,Li X,Lim K,Mohanram H,Peng L,Mishra B,Basu A,Lee JM,Bhattacharjya S,Leong SS

更新日期：2014-01-01 00:00:00

abstract：:Ethanol-producing bioreactors employing cells of Zymomonas mobilis attached to glass-fiber pads were operated continuously for as long as 28 days. Ethanol production, which is related to bed-associated biomass levels, was found to occur in three distinct phases: an exponential phase, a linear phase, and a "steady-stat...

journal_title：Biotechnology and bioengineering

authors： Arcuri EJ

更新日期：1982-03-01 00:00:00

abstract：:The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size wit...

journal_title：Biotechnology and bioengineering

authors： Chan MY,Hoare M,Dunnill P

更新日期：1986-03-01 00:00:00

abstract：:Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...

journal_title：Biotechnology and bioengineering

authors： Kamata K,Toya Y,Shimizu H

更新日期：2019-05-01 00:00:00

abstract：:Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

journal_title：Biotechnology and bioengineering

authors： Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

更新日期：2008-01-01 00:00:00

abstract：:The patterning of cardiac myocytes on a micron scale ( approximately 5 microm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca(2+) transition o...

journal_title：Biotechnology and bioengineering

authors： Kaji H,Takoh K,Nishizawa M,Matsue T

更新日期：2003-03-20 00:00:00

abstract：:Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved...

journal_title：Biotechnology and bioengineering

authors： Yoshimoto N,Ikeda Y,Tatematsu K,Iijima M,Nakai T,Okajima T,Tanizawa K,Kuroda S

更新日期：2016-08-01 00:00:00

abstract：:The mean sizes and size distributions of air bubbles and viscous castor oil drops were studied in a salt-rich aqueous solution (medium), first separately, and then simultaneously as a three-phase system. The dispersion was created in a 150-mm-diameter stirred tank equipped with a Rushton turbine, and the sizes were me...

journal_title：Biotechnology and bioengineering

authors： Galindo E,Pacek AW,Nienow AW

更新日期：2000-07-20 00:00:00

abstract：:A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Kozaki Y,Kodama H,Shioya S

更新日期：1999-03-05 00:00:00

abstract：:The initial steps of glycerol dissimilation and 1,3-propanediol (1, 3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The...

journal_title：Biotechnology and bioengineering

authors： Ahrens K,Menzel K,Zeng A,Deckwer W

更新日期：1998-09-05 00:00:00

abstract：:Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2-13 C]glucose or [U-13 C]glutamine to evaluate metabolic alterations in the amniocyte-derived, E1-transformed 1...

journal_title：Biotechnology and bioengineering

authors： Carinhas N,Koshkin A,Pais DA,Alves PM,Teixeira AP

更新日期：2017-01-01 00:00:00

abstract：:Fed-batch cultures of recombinant microorganisms have attracted attention as they can separate cell growth stage from cloned-gene expression phase during fermentations. In this work, the effect of different glucose feeding strategies on cell growth and cloned gene expression was studied during aerobic fed-batch fermen...

journal_title：Biotechnology and bioengineering

authors： Patkar A,Seo JH

更新日期：1992-06-05 00:00:00

abstract：:Screening tests of different marine algae biomas types revealed a high passive biosorptive uptake of lead up to 270 mg Pb/g of biomass in some brown marine algae. Members of the order Fucales perfomed particularly well in this descending sequence: Fucus > Ascophyllum > Sargassum. Although decreasing the swelling of we...

journal_title：Biotechnology and bioengineering

authors： Holan ZR,Volesky B

更新日期：1994-05-01 00:00:00

abstract：:We investigated the enzymic activity of bilirubin oxidase when it is suspended as a lyophilized powder in a low-water system. The enzyme required buffer salts and a source of water to show activity. This study investigated the complete range of water thermodynamic activity (a(w)) by combining the use of salt hydrates ...

journal_title：Biotechnology and bioengineering

authors： Alston MJ,Freedman RB

更新日期：2002-03-20 00:00:00

abstract：:The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (...

journal_title：Biotechnology and bioengineering

authors： Rossouw CL,Chetty A,Moolman FS,Birkholtz LM,Hoppe H,Mancama DT

更新日期：2012-08-01 00:00:00

abstract：:Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...

journal_title：Biotechnology and bioengineering

authors： Vaidya AA,Lele BS,Kulkarni MG,Mashelkar RA

更新日期：1999-08-20 00:00:00

abstract：:Regioselectivity of glycosyltransferases offers an important means to overcome the limitations of chemical synthesis of small molecule glycosides. In this study we explore a large multigene family of UDP-glucose:glycosyltransferases of Arabidopsis for their potential as novel biocatalysts for in vitro synthesis and wh...

journal_title：Biotechnology and bioengineering

authors： Lim EK,Ashford DA,Hou B,Jackson RG,Bowles DJ

更新日期：2004-09-05 00:00:00

abstract：:Model experiments were performed with starved yeast (Saccharomyces cerevisiae) cultures in a batch reactor in order to develop a better understanding of NAD(P)H and flavin culture fluorescence. Fluorescence was monitored during aerobic-anaerobic-aerobic transitions and ethanol and glucose substrate addition experiment...

journal_title：Biotechnology and bioengineering

authors： Siano SA,Mutharasan R

更新日期：1989-08-20 00:00:00

abstract：:Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed)...

journal_title：Biotechnology and bioengineering

authors： Wu LQ,Chen T,Wallace KK,Vazquez-Duhalt R,Payne GF

更新日期：2001-12-01 00:00:00

abstract：:An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hy...

journal_title：Biotechnology and bioengineering

authors： Heumann S,Eberl A,Fischer-Colbrie G,Pobeheim H,Kaufmann F,Ribitsch D,Cavaco-Paulo A,Guebitz GM

更新日期：2009-03-01 00:00:00

abstract：:Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...

journal_title：Biotechnology and bioengineering

authors： Ko JK,Kim Y,Ximenes E,Ladisch MR

更新日期：2015-02-01 00:00:00

abstract：:Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

journal_title：Biotechnology and bioengineering

authors： Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

更新日期：2019-01-01 00:00:00