Intracellular Ca2+ imaging for micropatterned cardiac myocytes.

abstract：:A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...

journal_title：Biotechnology and bioengineering

authors： Pathi B,Kinsey ST,Locke BR

更新日期：2011-08-01 00:00:00

abstract：:In this study, we assessed the importance of cytoskeleton organization in the mammalian cells used to produce therapeutic proteins. Two cytoskeletal genes, Actin alpha cardiac muscle 1 (ACTC1) and a guanosine triphosphate GTPase-activating protein (TAGAP), were found to be upregulated in highly productive therapeutic ...

journal_title：Biotechnology and bioengineering

authors： Pourcel L,Buron F,Arib G,Le Fourn V,Regamey A,Bodenmann I,Girod PA,Mermod N

更新日期：2020-04-01 00:00:00

abstract：:The rate of celluose degradation, limited due to the inhibition by cellobiose, can be increased by the hydrolysis of cellobiose to glucose using immobilized beta-glucosidase. Production of beta-glucosidase in four yeasts was studied and a maximum activity of 1.22 IU/mg cells was obtained in cells of Pichia etchellsii ...

journal_title：Biotechnology and bioengineering

authors： Jain D,Ghose TK

更新日期：1984-04-01 00:00:00

abstract：:An 11-L helical ribbon impeller (HRI) bioreactor was tested for the culture of Spodoptera frugiperda (Sf-9) cells. This impeller and surface baffling ensured homogeneous mixing and high oxygen transfer through surface aeration and surface-induced babble generation. Serum-supplemented and serum-free cultures, using TNM...

journal_title：Biotechnology and bioengineering

authors： Kamen AA,Tom RL,Caron AW,Chavarie C,Massie B,Archambault J

更新日期：1991-09-01 00:00:00

abstract：:The flotation of Streptomyces pilosus was studied in the absence and presence of adsorbed Pb(II) ions on the cell walls and with and without using sodium dodecylsulfate (SDS) as collector. Adsorption of SDS onto the organism in the absence and presence of Pb(II) ions was also investigated. It was found that in the abs...

journal_title：Biotechnology and bioengineering

authors： Sadowski Z,Golab Z,Smith RW

更新日期：1991-04-25 00:00:00

abstract：:Benzil was reduced stereospecifically to (S)-benzoin by Bacillus cereus strain Tim-r01. To isolate the gene responsible for asymmetric reduction, we constructed a library consisting of Escherichia coli clones that harbored plasmids expressing Bacillus cereus genes. The library was screened using the halo formation ass...

journal_title：Biotechnology and bioengineering

authors： Maruyama R,Nishizawa M,Itoi Y,Ito S,Inoue M

更新日期：2001-12-20 00:00:00

abstract：:The first genome scale model (GSM) for Streptomyces leeuwenhoekii C34 was developed to study the biosynthesis pathways of specialized metabolites and to find metabolic engineering targets for enhancing their production. The model, iVR1007, consists of 1,722 reactions, 1,463 metabolites, and 1,007 genes, it includes th...

journal_title：Biotechnology and bioengineering

authors： Razmilic V,Castro JF,Andrews B,Asenjo JA

更新日期：2018-07-01 00:00:00

abstract：:In the present investigation molecular sieve has been found to be a suitable carrier for the immobilization of enzymes. From the present study it may be specifically found that trypsin and pappain can be immobilized by molecular sieve type 4a following a very simple techniqure. The immobilized enzyme can be used both ...

journal_title：Biotechnology and bioengineering

authors： Mukherjea RN,Bhattacharya P,Ghosh BK,Taraphdar DK

更新日期：1977-09-01 00:00:00

abstract：:Being biosurfactants, rhamnolipids create severe foaming when produced in aerobic Pseudomonas aeruginosa fermentation. The necessary reduction of aeration causes oxygen limitation and restricts cell and product concentrations. In this study, we evaluate the new strategy of rhamnolipid production under denitrification ...

journal_title：Biotechnology and bioengineering

authors： Chayabutra C,Wu J,Ju LK

更新日期：2001-01-05 00:00:00

abstract：:The S-System formalism provides a popular, versatile and mathematically tractable representation of metabolic pathways. At steady-state, after a logarithmic transformation, the S-System representation reduces into a system of linear equations. Thus, the maximization of a particular metabolite concentration or a flux s...

journal_title：Biotechnology and bioengineering

authors： Petkov SB,Maranas CD

更新日期：1997-10-20 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:Three-dimensional (3D) tumor models have been established in various microfluidic systems for drug delivery and resistance studies in vitro. However, one of the main drawbacks of these models is non-uniform distribution of cells, leaving regions with very low cell density within the 3D structures. As a result, molecul...

journal_title：Biotechnology and bioengineering

authors： Elliott NT,Yuan F

更新日期：2012-05-01 00:00:00

abstract：:We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffol...

journal_title：Biotechnology and bioengineering

authors： Tiğli RS,Gümüşderelioğlu M

更新日期：2009-10-15 00:00:00

abstract：:This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of ...

journal_title：Biotechnology and bioengineering

authors： McMahon M,Mulcahy P

更新日期：2002-03-05 00:00:00

abstract：:Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...

journal_title：Biotechnology and bioengineering

authors： Ren H,Hu P,Zhao H

更新日期：2017-08-01 00:00:00

abstract：:A mixed culture of sulfate-reducing bacteria containing the species Desulfovibrio desulfuricans was used to study sulfate-reduction stoichiometry and kinetics using ethanol as the carbon source. Growth yield was lower, and kinetics were slower, for ethanol compared to lactate. Ethanol was converted into acetate and no...

journal_title：Biotechnology and bioengineering

authors： Nagpal S,Chuichulcherm S,Livingston A,Peeva L

更新日期：2000-12-05 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00

abstract：:Saccharomyces cerevisiae is a widely used host organism for the production of heterologous proteins, often cultivated in glucose-based fed-batch processes. This production system however has many factors limiting the productivity, mainly towards the end of the fermentation. For the optimised production of a Camelid an...

journal_title：Biotechnology and bioengineering

authors： van de Laar T,Visser C,Holster M,López CG,Kreuning D,Sierkstra L,Lindner N,Verrips T

更新日期：2007-02-15 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:High throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput ap...

journal_title：Biotechnology and bioengineering

authors： Li H,Gao X,Demartini JD,Kumar R,Wyman CE

更新日期：2013-11-01 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...

journal_title：Biotechnology and bioengineering

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-03-20 00:00:00

abstract：:Steam explosion after sulphur dioxide impregnation of wood chips is an effective method for improving the enzymatic digestibility of cellulose in the softwood Pinus radiata. Digestibility of pretreated fiber was progressively increased by altering the conditions of steam explosion. With increasing digestibility, there...

journal_title：Biotechnology and bioengineering

authors： Wong KK,Deverell KF,Mackie KL,Clark TA,Donaldson LA

更新日期：1988-04-05 00:00:00

abstract：:Oxygenases catalyze, among other interesting reactions, highly selective hydrocarbon oxyfunctionalizations, which are important in industrial organic synthesis but difficult to achieve by chemical means. Many enzymatic oxygenations have been described, but few of these have been scaled up to industrial scales, due to ...

journal_title：Biotechnology and bioengineering

authors： Bühler B,Bollhalder I,Hauer B,Witholt B,Schmid A

更新日期：2003-06-30 00:00:00

abstract：:This study deals with the use of an upward gas/liquid slug flow to reduce tubular mineral membrane fouling. The injection of air into the feedstream is designed to create hydrodynamic conditions that destabilize the cake layer over the membrane surface inside the filtration module complex. Experimental study was carri...

journal_title：Biotechnology and bioengineering

authors： Mercier M,Maranges C,Fonade C,Lafforgue-Delorme C

更新日期：1998-04-05 00:00:00

abstract：:Reducing viscosities of concentrated solutions of therapeutic proteins is important for their subcutaneous and intravenous delivery. Although inorganic salts and optimizing the pH were previously reported to dramatically lower the viscosity of a monoclonal antibody solution, herein we have determined these effects not...

journal_title：Biotechnology and bioengineering

authors： Du W,Klibanov AM

更新日期：2011-03-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are characterized by a low glucose catabolic efficiency, resulting in undesirable lactate production. Here, it is hypothesized that such low efficiency is determined by the transport of pyruvate into the mitochondria. The mitochondrial pyruvate carrier (MPC), responsible for introduci...

journal_title：Biotechnology and bioengineering

authors： Bulté DB,Palomares LA,Parra CG,Martínez JA,Contreras MA,Noriega LG,Ramírez OT

更新日期：2020-09-01 00:00:00

abstract：:A bioremediation process to remove Co(2+) from aqueous solution is investigated in this study using a magnetic resonance imaging (MRI) protocol to rapidly obtain multiple 2D spatially resolved Co(2+) ion concentration maps. The MRI technique is described in detail and its ability to determine the evolution in both axi...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Holland DJ,Paterson-Beedle M,Macaskie LE,Gladden LF,Johns ML

更新日期：2008-03-01 00:00:00

abstract：:Autoflocculation and bioflocculation are considered to be the most promising means for the economical harvesting of microalgae. We have therefore studied these phenomena with cultures of Scenedesmus obliquus produced during biological tertiary wastewater treatment. The quantity of extracellular polymers produced durin...

journal_title：Biotechnology and bioengineering

authors： Lavoie A,de la Noüe J

更新日期：1987-11-01 00:00:00

abstract：:A novel two-stage bioreactor has been designed for a combined submerged (SF) and solid substrate fermentation (SSF) of wheat straw. The straw was pretreated with steam, and cellulases from the culture fluid of Trichoderma reesei were adsorbed on it for increased bioconvertibility. SSF was conducted in the top part of ...

journal_title：Biotechnology and bioengineering

authors： Viesturs UE,Strikauska SV,Leite MP,Berzins AJ,Tengerdy RP

更新日期：1987-08-05 00:00:00