Increased heterologous protein production by Saccharomyces cerevisiae growing on ethanol as sole carbon source.

abstract：:In large-scale mammalian cell culture, the key toxic by-products assessed and monitored are lactate and ammonia. Often no distinction between the two isoforms of lactate is made. Here, we present profiles of both D- and L-lactate. D-Lactate is the end molecule of the methylglyoxal pathway. D-Lactate unlike L-lactate i...

journal_title：Biotechnology and bioengineering

authors： Paoli T,Faulkner J,O'kennedy R,Keshavarz-Moore E

更新日期：2010-09-01 00:00:00

abstract：:An examination is made of five methods for obtaining values of the enthalpy of formation of a unit mass of living Escherichia coli K-12 cells. The values obtained by these methods ranged from -88.95 kJ to -99.55 kJ, the gross average being 96.01 kJ, per unit carbon formula weight equivalent of living, hydrated cells. ...

journal_title：Biotechnology and bioengineering

authors： Battley EH

更新日期：1992-01-05 00:00:00

abstract：:In this study we describe a model that estimates the extracellular (nonfungal) and overall water contents of wheat grains during solid-state fermentation (SSF) with Aspergillus oryzae, using on-line measurements of oxygen, carbon dioxide, and water vapor in the gas phase. The model uses elemental balances to predict s...

journal_title：Biotechnology and bioengineering

authors： Nagel FJ,Tramper J,Bakker MS,Rinzema A

更新日期：2001-01-20 00:00:00

abstract：:A model was developed to evaluate the effects of cells and surfactants on oxygen transfer in surface-aerated bioreactors. The model assumed the presence of serial layers of adsorbed surfactants and microorganisms directly adjacent to the gas-liquid interface due to their surface activities, followed by a stagnant liqu...

journal_title：Biotechnology and bioengineering

authors： Ju LK,Sundarajan A

更新日期：1992-12-20 00:00:00

abstract：:Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...

journal_title：Biotechnology and bioengineering

authors： Landberg J,Wright NR,Wulff T,Herrgård MJ,Nielsen AT

更新日期：2020-08-18 00:00:00

abstract：:Shake flask and stirred tank bioleaching experiments showed that the dissolution of chalcopyrite is inhibited by ferric ion concentrations as low as 200 mg L(-1) and redox potentials >420 mV (vs. Ag/AgCl). Chemical leaching of chalcopyrite (4% suspension, surface area 2.3 m2 g(-1)) was enhanced fourfold in the presenc...

journal_title：Biotechnology and bioengineering

authors： Third KA,Cord-Ruwisch R,Watling HR

更新日期：2002-05-20 00:00:00

abstract：:Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...

journal_title：Biotechnology and bioengineering

authors： Hjortso MA,Bailey JE

更新日期：1984-05-01 00:00:00

abstract：:The damaging and degenerative effects in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and Crohn's disease often manifests as the formation of lesions that feature a high local concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). GM-CSF along with other pro-inflammatory fa...

journal_title：Biotechnology and bioengineering

authors： Mosabbir AA,Qudrat A,Truong K

更新日期：2018-04-01 00:00:00

abstract：:The production of acetate by aerobically growing E. coli is examined. The problem is formulated in terms of a flow network that has as its objective maximal ATP synthesis. It is found that when loads are imposed and flux constraints exist either at the level of NADH turnover rate or the activity of a key Krebs cycle e...

journal_title：Biotechnology and bioengineering

authors： Majewski RA,Domach MM

更新日期：1990-03-25 00:00:00

abstract：:Immobilized enzyme hollow fibers may be useful in the purification or treatment of whole blood under clinical conditions. In this study, catalytically pure heparinase was immobilized to cellulose to analyze the feasibility for the removal of heparin's anticoagulant activity from whole blood. The kinetics of catalytica...

journal_title：Biotechnology and bioengineering

authors： Comfort AR,Albert E,Langer R

更新日期：1989-12-20 00:00:00

abstract：:A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

journal_title：Biotechnology and bioengineering

authors： Wang M,Yu C,Zhao H

更新日期：2016-03-01 00:00:00

abstract：:Mechanical forces regulate the structure and function of many tissues in vivo; recent results indicate that the mechanical environment can decisively influence the development of engineered tissues cultured in vitro. To investigate the effects of the hydrodynamic environment on tissue-engineered cartilage, primary bov...

journal_title：Biotechnology and bioengineering

authors： Gooch KJ,Kwon JH,Blunk T,Langer R,Freed LE,Vunjak-Novakovic G

更新日期：2001-02-20 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:Enterokinase and recombinant enterokinase light chain (rEK(L)) have been used widely to cleave fusion proteins with the target sequence of (Asp)(4)-Lys. In this work, we show that their utility as a site-specific cleavage agent is compromised by sporadic cleavage at other sites, albeit at low levels. Further degradati...

journal_title：Biotechnology and bioengineering

authors： Choi SI,Song HW,Moon JW,Seong BL

更新日期：2001-12-20 00:00:00

abstract：:A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow-fiber ultrafiltration technology under conditions of large-scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5-fo...

journal_title：Biotechnology and bioengineering

authors： Weiss SA

更新日期：1980-01-01 00:00:00

abstract：:Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ tran...

journal_title：Biotechnology and bioengineering

authors： Perez-Basterrechea M,Esteban MM,Vega JA,Obaya AJ

更新日期：2018-12-01 00:00:00

abstract：:A two-member methane-utilizing mixed culture of bacteria, formed by combining two pure cultures isolated from a naturally occurring methane-utilizing mixed culture, was studied in continuous culture. From the nutritional requirements and substrate ranges of the pure cultures, a mechanism for the interspecific interact...

journal_title：Biotechnology and bioengineering

authors： Lamb SC,Garver JC

更新日期：1980-10-01 00:00:00

abstract：:Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of serum. Existing protocols desig...

journal_title：Biotechnology and bioengineering

authors： Jaluria P,Konstantopoulos K,Betenbaugh M,Shiloach J

更新日期：2008-04-15 00:00:00

abstract：:The use of recombinant adenoviral vectors for vaccination and gene therapy requires the development of purification processes that are cost-effective, scalable, and capable of robust host cell DNA clearance. An adenovirus purification process was developed which incorporates selective precipitation of host cell DNA, e...

journal_title：Biotechnology and bioengineering

authors： Goerke AR,To BC,Lee AL,Sagar SL,Konz JO

更新日期：2005-07-05 00:00:00

abstract：:Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of ...

journal_title：Biotechnology and bioengineering

authors： Sahoo S,Rao KK,Suraishkumar GK

更新日期：2006-05-05 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00

abstract：:We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell d...

journal_title：Biotechnology and bioengineering

authors： Echtenkamp PL,Wilson DB,Shuler ML

更新日期：2009-02-15 00:00:00

abstract：:Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...

journal_title：Biotechnology and bioengineering

authors： Jong JZ,Hsiun DY,Wu WT,Tzeng YM

更新日期：1995-11-05 00:00:00

abstract：:We investigated the role of green sulfur bacteria inlight-responsive electricity generation in microbial electrochemical cells (MXCs). We operated MXCs containing either monocultures or defined cocultures of previously enriched phototrophic Chlorobium and anode-respiring Geobacter under anaerobic conditions in the abs...

journal_title：Biotechnology and bioengineering

authors： Badalamenti JP,Torres CI,Krajmalnik-Brown R

更新日期：2014-02-01 00:00:00

abstract：:Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engine...

journal_title：Biotechnology and bioengineering

authors： Clomburg JM,Gonzalez R

更新日期：2011-04-01 00:00:00

abstract：:The flotation of Streptomyces pilosus was studied in the absence and presence of adsorbed Pb(II) ions on the cell walls and with and without using sodium dodecylsulfate (SDS) as collector. Adsorption of SDS onto the organism in the absence and presence of Pb(II) ions was also investigated. It was found that in the abs...

journal_title：Biotechnology and bioengineering

authors： Sadowski Z,Golab Z,Smith RW

更新日期：1991-04-25 00:00:00

abstract：:CHO cells express glycoproteins containing both the N-acetylneuraminic acid (Neu5Ac) and minor amounts of the N-glycolylneuraminic acid (Neu5Gc) forms of sialic acid. As Neu5Gc is not expressed in humans and can be recognized as a foreign epitope, there is the potential for immunogenicity issues for glycoprotein thera...

journal_title：Biotechnology and bioengineering

authors： Borys MC,Dalal NG,Abu-Absi NR,Khattak SF,Jing Y,Xing Z,Li ZJ

更新日期：2010-04-15 00:00:00

abstract：:The use of cell numbers rather than mass to quantify the size of the biotic phase in animal cell cultures causes several problems. First, the cell size varies with growth conditions, thus yields expressed in terms of cell numbers cannot be used in the normal mass balance sense. Second, experience from microbial system...

journal_title：Biotechnology and bioengineering

authors： Nielsen LK,Reid S,Greenfield PF

更新日期：1997-11-20 00:00:00

abstract：:Flocculation of yeast cells occurs during the fermentation of beer. Partway through the fermentation the cells become flocculent and start to form flocs. If the environmental conditions, such as medium composition and fluid velocities in the tank, are optimal, the flocs will grow in size large enough to settle. After ...

journal_title：Biotechnology and bioengineering

authors： van Hamersveld EH,van der Lans RG,Caulet PJ,Luyben KC

更新日期：1998-02-05 00:00:00

abstract：:The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. Here we use tetracyc...

journal_title：Biotechnology and bioengineering

authors： Umaña P,Jean-Mairet J,Bailey JE

更新日期：1999-12-05 00:00:00