Abstract:
:Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose concentration as the output variable was fed back to computer for calculation of substrate addition. The modified reactor was an airlift reactor with a net draft tube. The airlift reactor had high oxygen transfer rate and low shear stress which were important factors for production of thuringiensin. Fed-batch culture of Bacillus thuringiensis in the modified airlift reactor provided significant improvement of thuringiensin production.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Jong JZ,Hsiun DY,Wu WT,Tzeng YMdoi
10.1002/bit.260480307subject
Has Abstractpub_date
1995-11-05 00:00:00pages
207-13issue
3eissn
0006-3592issn
1097-0290journal_volume
48pub_type
杂志文章abstract::Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2-13 C]glucose or [U-13 C]glutamine to evaluate metabolic alterations in the amniocyte-derived, E1-transformed 1...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26063
更新日期:2017-01-01 00:00:00
abstract::Bioluminescence from the lux-based bacterial reporter Pseudomonas fluorescens HK44 was experimentally investigated under growth substrate-rich and limiting conditions in batch, continuous stirred tank (CSTR), and turbidostat reactors. A mechanistically based, mathematical model was developed to describe bioluminescenc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10475
更新日期:2003-02-05 00:00:00
abstract::The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resulting from lyophilizati...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990420)63:2<233::aid-bit
更新日期:1999-04-20 00:00:00
abstract::Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26839
更新日期:2019-01-01 00:00:00
abstract::As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24377
更新日期:2012-04-01 00:00:00
abstract::The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitri...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25866
更新日期:2016-05-01 00:00:00
abstract::A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10428
更新日期:2002-12-30 00:00:00
abstract::This paper describes batch and semicontinuous acetic acid fermentations for wine vinegar production carried out with Acetobacter pasteurianus, and an industrial strain using a noncommercial 100-L bubble column reactor equipped with a novel type of gas-liquid dynamic sparger. Results showed acetification rates with thi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990420)63:2<141::aid-bit
更新日期:1999-04-20 00:00:00
abstract::Understanding of the fundamental mechanisms that govern unintentional differentiation of human induced pluripotent stem cells (hiPSCs) provides key strategies to maintain their undifferentiated state during cell expansion. This study focused on deviation from the undifferentiated state in hiPSC colonies during culture...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25188
更新日期:2014-06-01 00:00:00
abstract::As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27311
更新日期:2020-06-01 00:00:00
abstract::Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26309
更新日期:2017-08-01 00:00:00
abstract::A comprehensive study of the application of continuous zone electrophoresis to preparative separation of proteins in free solution is presented. First, the influence of electric field strength, buffer residence time in the chamber, sample flow rate, and sample concentration on separation resolution and throughput were...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420707
更新日期:1993-09-20 00:00:00
abstract::A new method for solid-state ethanol fermentation (the SSEF system) was experimented on for the ethanol production from solid starchy materials, where a packedbed-type fermentor was used. Both cultivation of Aspergillus saitoi and enrichment of a saccharifying enzyme were effective for hydrolysis of the starch. Ethano...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270907
更新日期:1985-09-01 00:00:00
abstract::Mass transfer is known to play a critical role in bioprocess performance and henceforth monitoring dissolved O2 (DO) and dissolved CO2 (dCO2 ) is of paramount importance. At bioreactor level these parameters can be monitored online and can be controlled by sparging air/oxygen or stirrer speed. However, traditional sma...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27252
更新日期:2020-04-01 00:00:00
abstract::Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990820)64:4<418::aid-bit
更新日期:1999-08-20 00:00:00
abstract::In macroscopic dynamic models of fermentation processes, elementary modes (EM) derived from metabolic networks are often used to describe the reaction stoichiometry in a simplified manner and to build predictive models by parameterizing kinetic rate equations for the EM. In this procedure, the selection of a set of EM...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27340
更新日期:2020-07-01 00:00:00
abstract::To investigate the effects of factors secreted by different cell lines on human monoclonal antibody (MAb) integrity, 600 mg of a human MAb, which specifically binds to human erythrocytes, were produced in a perfusion process. After purification by protein A affinity chromatography, the MAb was used for integrity testi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260450202
更新日期:1995-01-20 00:00:00
abstract::A gene coding for human parathyroid hormone (hPTH) was synthesized and cloned into a yeast expression and secretion vector containing the mating factor alpha pre-pro leader sequence and the galactose-inducible promoter, GAL10. The intact hPTH(1-84) was found to be secreted into the culture medium. As observed in the p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980120)57:2<245::aid-bit
更新日期:1998-01-20 00:00:00
abstract::The use of recombinant adenoviral vectors for vaccination and gene therapy requires the development of purification processes that are cost-effective, scalable, and capable of robust host cell DNA clearance. An adenovirus purification process was developed which incorporates selective precipitation of host cell DNA, e...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20406
更新日期:2005-07-05 00:00:00
abstract::The use of expanded beds of STREAMLINE ion exchange adsorbents for the direct extraction of an intracellular enzyme glucose-6-phosphate dehydrogenase (G6PDH) from unclarified yeast cell homogenates has been investigated. It has been demonstrated that such crude feedstocks can be applied to the bed without prior clarif...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960120)49:2<204::AID-BIT
更新日期:1996-01-20 00:00:00
abstract::Protein-protein interactions were measured for ovalbumin and for lysozyme in aqueous salt solutions. Protein-protein interactions are correlated with a proposed potential of mean force equal to the free energy to desolvate the protein surface that is made inaccessible to the solvent due to the protein-protein interact...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10342
更新日期:2002-08-20 00:00:00
abstract::The application of the unscented Kalman filter to control starvation-induced programmed cell death-apoptosis-in Chinese hamster ovary cells was investigated. Neural network-based sensitivity analysis identified glutamine and asparagine as two major amino acids that play a key role in the suppression of apoptosis. Dyna...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10250
更新日期:2002-06-20 00:00:00
abstract::In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251222
更新日期:1983-12-01 00:00:00
abstract::The ferrous-iron oxidation kinetics of a bacterial culture consisting predominantly of Leptospirillum ferrooxidans were studied in continuous-flow bioreactors. The bacterial culture was fed with a salts solution containing 12 g/L ferrous-iron, at dilution rates ranging from 0.01 to 0.06 l/h, and temperatures ranging f...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19991005)65:1<44::aid-bit6
更新日期:1999-10-05 00:00:00
abstract::The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Syn...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25712
更新日期:2016-02-01 00:00:00
abstract::Highly branched mutants of two strains of Aspergillus oryzae (IFO4177, which produces alpha-amylase, and a transformant of IFO4177 [AMG#13], which produces heterologous glucoamylase in addition to alpha-amylase) were generated by UV or nitrous acid mutagenesis. Four mutants of the parental strain (IFO4177), which were...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19991220)65:6<638::aid-bit
更新日期:1999-12-20 00:00:00
abstract::Based on the recently constructed Escherichia coli itaconic acid production strain ita23, we aimed to improve the productivity by applying a two-stage process strategy with decoupled production of biomass and itaconic acid. We constructed a strain ita32 (MG1655 ΔaceA Δpta ΔpykF ΔpykA pCadCs), which, in contrast to ita...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26446
更新日期:2018-01-01 00:00:00
abstract::The success of in situ bioremediation is often limited by the inability to bring bacteria in contact with the pollutant, which they will degrade. A bench-scale model aquifer was used to evaluate the impact of chemotaxis on the migration of bacteria toward the source of a chemical pollutant. The model was packed with s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23159
更新日期:2011-09-01 00:00:00
abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25841
更新日期:2016-04-01 00:00:00
abstract::Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global n...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22860
更新日期:2010-12-15 00:00:00