Tetracycline-regulated overexpression of glycosyltransferases in Chinese hamster ovary cells.

abstract：:In this study it is reported the operation of a horizontal-flow anaerobic immobilized biomass (HAIB) reactor under sulfate-reducing condition which was also exposed to different amounts of ethanol and toluene. The system was inoculated with sludge taken from up-flow anaerobic sludge blanket (UASB) reactors treating re...

journal_title：Biotechnology and bioengineering

authors： Cattony EB,Chinalia FA,Ribeiro R,Zaiat M,Foresti E,Varesche MB

更新日期：2005-07-20 00:00:00

abstract：:Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

journal_title：Biotechnology and bioengineering

authors： Davidson AJ,Ellis MJ,Chaudhuri JB

更新日期：2010-08-15 00:00:00

abstract：:In regenerative medicine, computer models describing bioreactor processes can assist in designing optimal process conditions leading to robust and economically viable products. In this study, we started from a (3D) mechanistic model describing the growth of neotissue, comprised of cells, and extracellular matrix, in a...

journal_title：Biotechnology and bioengineering

authors： Mehrian M,Guyot Y,Papantoniou I,Olofsson S,Sonnaert M,Misener R,Geris L

更新日期：2018-03-01 00:00:00

abstract：:Refiner mechanical pulp was biologically treated with several higher fungi in order to test their potential for increasing the strength of paper. It was among the white-rot fungi that the best results were obtained. Polyporus versicolor gave the best overall improvement in handsheet properties with no reduction in tea...

journal_title：Biotechnology and bioengineering

authors： Pilon L,Barbe MC,Desrochers M,Jurasek L,Neumann PJ

更新日期：1982-09-01 00:00:00

abstract：:A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

journal_title：Biotechnology and bioengineering

authors： Goey CH,Tsang JMH,Bell D,Kontoravdi C

更新日期：2017-12-01 00:00:00

abstract：:In large-scale mammalian cell culture, the key toxic by-products assessed and monitored are lactate and ammonia. Often no distinction between the two isoforms of lactate is made. Here, we present profiles of both D- and L-lactate. D-Lactate is the end molecule of the methylglyoxal pathway. D-Lactate unlike L-lactate i...

journal_title：Biotechnology and bioengineering

authors： Paoli T,Faulkner J,O'kennedy R,Keshavarz-Moore E

更新日期：2010-09-01 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...

journal_title：Biotechnology and bioengineering

authors： James RI,Elton JP,Todd P,Kompala DS

更新日期：2000-01-20 00:00:00

abstract：:Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell sorting (FACS). For comparison, cell pools and cell lines were al...

journal_title：Biotechnology and bioengineering

authors： Oberbek A,Matasci M,Hacker DL,Wurm FM

更新日期：2011-03-01 00:00:00

abstract：:Metastatic disease remains one of the primary reasons for cancer-related deaths, yet the majority of in vitro cancer models focus on the primary tumor sites. Here, we describe a metastasis-on-a-chip device that houses multiple bioengineered three-dimensional (3D) organoids, established by a 3D photopatterning techniqu...

journal_title：Biotechnology and bioengineering

authors： Aleman J,Skardal A

更新日期：2019-04-01 00:00:00

abstract：:Potentially damaging reactive oxygen species (ROS) are involved in a number of pathways ranging from signal transduction to apoptosis. Cells have adapted this alteration in redox status into a complex regulatory mechanism. ROS are specifically able to induce the expression of a multitude of genes. We constructed and c...

journal_title：Biotechnology and bioengineering

authors： Lu C,Albano CR,Bentley WE,Rao G

更新日期：2005-03-05 00:00:00

abstract：:As nitrogen discharge limits are becoming more stringent, short-cut nitrogen systems and tertiary nitrogen polishing steps are gaining popularity. For partial nitritation or nitritation-denitritation systems, anaerobic ammonia oxidation (anammox) polishing may be feasible to remove residual ammonia and nitrite from th...

journal_title：Biotechnology and bioengineering

authors： Regmi P,Holgate B,Miller MW,Park H,Chandran K,Wett B,Murthy S,Bott CB

更新日期：2016-03-01 00:00:00

abstract：:The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...

journal_title：Biotechnology and bioengineering

authors： Ghose TK,Sahai V

更新日期：1979-02-01 00:00:00

abstract：:Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...

journal_title：Biotechnology and bioengineering

authors： Li Z,Cai Z,Fu W,Liu Y,Tian C,Wang H,Fu T,Wu Z,Wu D,Jin Y,Cheng Z,Terada N,Liu L,Wu W,Jin S,Bai F

更新日期：2020-03-01 00:00:00

abstract：:The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were ev...

journal_title：Biotechnology and bioengineering

authors： Dick LW Jr,Qiu D,Mahon D,Adamo M,Cheng KC

更新日期：2008-08-15 00:00:00

abstract：:Tissue function is comprised of a complex interplay between biological and physicochemical rate processes. The design of bioreactors for tissue engineering must account for these processes simultaneously in order to obtain a bioreactor that provides a uniform environment for tissue growth and development. In the prese...

journal_title：Biotechnology and bioengineering

authors： Peng CA,Palsson BØ

更新日期：1996-06-05 00:00:00

abstract：:The thickness variability of biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thick...

journal_title：Biotechnology and bioengineering

authors： Murga R,Stewart PS,Daly D

更新日期：1995-03-20 00:00:00

abstract：:The starting point for the present investigations was the finding that increasing influent concentrations from 10 to 380 mmol/L glucose decreased the attainable growth rate of an acidogenic population in continuous culture from 0.52 to 0.05 h(-1) To account for this phenomenon, a new kinetic model is developed that co...

journal_title：Biotechnology and bioengineering

authors： Van Den Heuvel JC,Beeftink HH

更新日期：1988-05-01 00:00:00

abstract：:Oxygen and nutrient limitation was investigated in order to identify the origin of a lower specific ajmalicine production in Catharanthus roseus cultures at high cell densities in an induction medium. The effect of oxygen limitation was explored by comparing two identically aerated and agitated high cell density biore...

journal_title：Biotechnology and bioengineering

authors： Schlatmann JE,Moreno PR,Vinke JL,Ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1994-08-05 00:00:00

abstract：:In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...

journal_title：Biotechnology and bioengineering

authors： Turner NA,Needs EC,Khan JA,Vulfson EN

更新日期：2001-01-05 00:00:00

abstract：:A perfusion culture system was developed to investigate the oxygenation of high-density hybridoma cell cultures. The culture system was composed of a stirred-tank bioreactor and an external microfiltration hollow fiber cartridge for medium perfusion. Cell growth and antibody production were examined with large bubble ...

journal_title：Biotechnology and bioengineering

authors： Zhang S,Handa-Corrigan A,Spier RE

更新日期：1993-03-25 00:00:00

abstract：:To monitor gas reaction rates in animal cell culture at constant dissolved oxygen concentration (DO) and constant pH it was necessary to develop improved control methods. Decoupling of both controllrs was obtained by manipulation of molar fractions of oxygen and carbon dioxide in the gas phase. Two pairs of DO and pH ...

journal_title：Biotechnology and bioengineering

authors： Oeggerli A,Eyer K,Heinzle E

更新日期：1995-01-05 00:00:00

abstract：:This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

journal_title：Biotechnology and bioengineering

authors： Dos Santos LM,Livingston AG

更新日期：1995-07-05 00:00:00

abstract：:Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...

journal_title：Biotechnology and bioengineering

authors： Yu J,Wang J

更新日期：2001-06-20 00:00:00

abstract：:Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their surface topography. Immobilizations were per...

journal_title：Biotechnology and bioengineering

authors： Wasserman BP,Hultin HO,Jacobson BS

更新日期：1980-02-01 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00

abstract：:The kinetics and mechanism of Fe(III) reduction to Fe(II) were studied in pure batch cultures of Pseudomonas sp. 200. The rate of iron reduction has been mechanistically related to aqueous phase iron speciation. In the absence of microbial activity the iron reduction rate was negligible. Initial rates of microbial iro...

journal_title：Biotechnology and bioengineering

authors： Arnold RG,Olson TM,Hoffmann MR

更新日期：1986-11-01 00:00:00

abstract：:Membrane morphology is compared to protein depostion under passive adsorption and ultrafiltration conditions. Solute resistance of protein deposits for membranes of varying roughness, structure, and permeability can vary dramatically with operating conditions. Using Brunauer-Emmett-Teller adsorption isotherm (BET), st...

journal_title：Biotechnology and bioengineering

authors： Chen V,Kim KJ,Fane AG

更新日期：1995-07-20 00:00:00

abstract：:A detailed mechanistic model of enzymatic cellulose hydrolysis has been developed. The behavior of individual cellulase enzymes and parameters describing the cellulose surface properties are included. Results obtained for individual enzymes (T. reesei endoglucanase 2 and cellobiohydrolase I) and systems with both enzy...

journal_title：Biotechnology and bioengineering

authors： Levine SE,Fox JM,Blanch HW,Clark DS

更新日期：2010-09-01 00:00:00

abstract：:A mutant of Saccharomyces cerevisiae, which forms large, multicellular flocs in liquid culture, rapidly fermented media containing high concentrations of glucose (100-180 g/L) in a continuous nonaerated tower fermentor at 30 degrees C. The fermentor operated continuously for seven months. Batch and tower fermentor dat...

journal_title：Biotechnology and bioengineering

authors： Jones ST,Korus RA,Admassu W,Heimsch RC

更新日期：1984-07-01 00:00:00

abstract：:Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...

journal_title：Biotechnology and bioengineering

authors： Arain S,Weiss S,Heinzle E,John GT,Krause C,Klimant I

更新日期：2005-05-05 00:00:00