High-yield method for immobilization of enzymes.

Abstract:

:Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their surface topography. Immobilizations were performed by adsorption followed by treatment with glutaraldehyde. The immobilized-enzyme activities per unit support of all of the enzymes tested were compared with and found to be superior to the immobilized activities attainable on aminopropyl-activated glass microbeads. When enzyme was present in less than saturating amounts, the coated beads were able to remove 100% of the glucose oxidase activity initially present in the immobilization solution, with 78-87% of that activity expressed on the support surface. Bound glucose oxidase was more stable to thermal inactivation than native enzyme.

journal_name

Biotechnol Bioeng

authors

Wasserman BP,Hultin HO,Jacobson BS

doi

10.1002/bit.260220203

subject

Has Abstract

pub_date

1980-02-01 00:00:00

pages

271-87

issue

2

eissn

0006-3592

issn

1097-0290

journal_volume

22

pub_type

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