Abstract:
:Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their surface topography. Immobilizations were performed by adsorption followed by treatment with glutaraldehyde. The immobilized-enzyme activities per unit support of all of the enzymes tested were compared with and found to be superior to the immobilized activities attainable on aminopropyl-activated glass microbeads. When enzyme was present in less than saturating amounts, the coated beads were able to remove 100% of the glucose oxidase activity initially present in the immobilization solution, with 78-87% of that activity expressed on the support surface. Bound glucose oxidase was more stable to thermal inactivation than native enzyme.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Wasserman BP,Hultin HO,Jacobson BSdoi
10.1002/bit.260220203subject
Has Abstractpub_date
1980-02-01 00:00:00pages
271-87issue
2eissn
0006-3592issn
1097-0290journal_volume
22pub_type
杂志文章abstract::The G-H loop of foot-and-mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine-glycine-aspartic acid tripeptide, a recognized integrin-binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide repr...
journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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