A comparison of oxygenation methods fro high-density perfusion culture of animal cells.

abstract：:We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffol...

journal_title：Biotechnology and bioengineering

authors： Tiğli RS,Gümüşderelioğlu M

更新日期：2009-10-15 00:00:00

abstract：:The production of volatile industrial chemicals utilizing metabolically engineered extreme thermophiles offers the potential for processes with simultaneous fermentation and product separation. An excellent target chemical for such a process is acetone (Tb  = 56°C), ideally produced from lignocellulosic biomass. Caldi...

journal_title：Biotechnology and bioengineering

authors： Straub CT,Bing RG,Otten JK,Keller LM,Zeldes BM,Adams MWW,Kelly RM

更新日期：2020-08-08 00:00:00

abstract：:After injury to the central nervous system, a glial scar develops that physically and biochemically inhibits axon growth. In the scar, activated astrocytes secrete inhibitory extracellular matrix, of which chondroitin sulfate proteoglycans (CSPGs) are considered the major inhibitory component. An inhibitory interface ...

journal_title：Biotechnology and bioengineering

authors： Tuinstra HM,Ducommun MM,Briley WE,Shea LD

更新日期：2013-03-01 00:00:00

abstract：:Flocculation of yeast cells occurs during the fermentation of beer. Partway through the fermentation the cells become flocculent and start to form flocs. If the environmental conditions, such as medium composition and fluid velocities in the tank, are optimal, the flocs will grow in size large enough to settle. After ...

journal_title：Biotechnology and bioengineering

authors： van Hamersveld EH,van der Lans RG,Caulet PJ,Luyben KC

更新日期：1998-02-05 00:00:00

abstract：:Highly substituted polychlorinated biphenyls (PCBs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. Functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by DNA shuffling and staggered extension process (StEP) of th...

journal_title：Biotechnology and bioengineering

authors： Brühlmann F,Chen W

更新日期：1999-06-05 00:00:00

abstract：:Application of the well-characterized Streptococcus pyogenes CRISPR-Cas9 system in actinomycetes streptomycetes has enabled high-efficiency multiplex genome editing and CRISPRi-mediated transcriptional regulation in these prolific bioactive metabolite producers. Nonetheless, SpCas9 has its limitations and can be ineff...

journal_title：Biotechnology and bioengineering

authors： Yeo WL,Heng E,Tan LL,Lim YW,Lim YH,Hoon S,Zhao H,Zhang MM,Wong FT

更新日期：2019-09-01 00:00:00

abstract：:Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ tran...

journal_title：Biotechnology and bioengineering

authors： Perez-Basterrechea M,Esteban MM,Vega JA,Obaya AJ

更新日期：2018-12-01 00:00:00

abstract：:Saccharomyces cerevisiae is a widely used host organism for the production of heterologous proteins, often cultivated in glucose-based fed-batch processes. This production system however has many factors limiting the productivity, mainly towards the end of the fermentation. For the optimised production of a Camelid an...

journal_title：Biotechnology and bioengineering

authors： van de Laar T,Visser C,Holster M,López CG,Kreuning D,Sierkstra L,Lindner N,Verrips T

更新日期：2007-02-15 00:00:00

abstract：:This study describes the development of a transient expression system for CHO cells based on autonomous replication and retention of transfected plasmid DNA. A transient expression system that allows extrachromosomal amplification of plasmids permits more plasmid copies to persist in the transfected cell throughout th...

journal_title：Biotechnology and bioengineering

authors： Kunaparaju R,Liao M,Sunstrom NA

更新日期：2005-09-20 00:00:00

abstract：:Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...

journal_title：Biotechnology and bioengineering

authors： Livernoche D,Jurasek L,Desrochers M,Dorica J,Veliky IA

更新日期：1983-08-01 00:00:00

abstract：:Recently, the respiratory quotient (RQ) of microbes measured in situ in a fermentor by exit-gas analysis has been used successfully, for instance, in a fed-batch culture of baker's yeast as a criterion to control the feeding rate.(1-3) It is significant here to keep RQ values close to unity throughout; any deviations ...

journal_title：Biotechnology and bioengineering

authors： Aiba S,Furuse H

更新日期：1990-08-20 00:00:00

abstract：:A computer-controlled headspace gas chromatograph was used to monitor the progress of ethanol production from both aerobic batch and anaerobic continuous fermentations. Using an automatic, electropneumatic sampling system, aliquots of fermentation headspace gas were injected directly onto the column for quantitative e...

journal_title：Biotechnology and bioengineering

authors： Comberbach DM,Bu'lock JD

更新日期：1983-11-01 00:00:00

abstract：:Mathematical modeling of animal cell growth and metabolism is essential for the understanding and improvement of the production of biopharmaceuticals. Models can explain the dynamic behavior of cell growth and product formation, support the identification of the most relevant parameters for process design, and signifi...

journal_title：Biotechnology and bioengineering

authors： Ramos JRC,Rath AG,Genzel Y,Sandig V,Reichl U

更新日期：2020-05-01 00:00:00

abstract：:In metabolic engineering, systems which allow coordinated control of two metabolic pathways can be useful. We designed two expression systems and demonstrated their application by coordinating glycogen synthesis and degradation. The first expression vector pMSW2 expressed the glycogen synthesis genes in one operon and...

journal_title：Biotechnology and bioengineering

authors： Dedhia N,Chen W,Bailey JE

更新日期：1997-07-20 00:00:00

abstract：:MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...

journal_title：Biotechnology and bioengineering

authors： Diendorfer AB,Hackl M,Klanert G,Jadhav V,Reithofer M,Stiefel F,Hesse F,Grillari J,Borth N

更新日期：2015-07-01 00:00:00

abstract：:In macroscopic dynamic models of fermentation processes, elementary modes (EM) derived from metabolic networks are often used to describe the reaction stoichiometry in a simplified manner and to build predictive models by parameterizing kinetic rate equations for the EM. In this procedure, the selection of a set of EM...

journal_title：Biotechnology and bioengineering

authors： Hebing L,Neymann T,Engell S

更新日期：2020-07-01 00:00:00

abstract：:Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitab...

journal_title：Biotechnology and bioengineering

authors： Paul AJ,Handrick R,Ebert S,Hesse F

更新日期：2018-05-01 00:00:00

abstract：:A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...

journal_title：Biotechnology and bioengineering

authors： Sadana A,Henley JP

更新日期：1987-10-20 00:00:00

abstract：:Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of ...

journal_title：Biotechnology and bioengineering

authors： Sahoo S,Rao KK,Suraishkumar GK

更新日期：2006-05-05 00:00:00

abstract：:Biofouling is the undesirable adhesion and development of microorganisms and macroorganisms in a water environment. An electrochemical antifouling system based on management of primary adhesion of microorganisms was developed employing titanium electrode for antifouling of seawater cooling pipes and marine infrastruct...

journal_title：Biotechnology and bioengineering

authors： Wake H,Takahashi H,Takimoto T,Takayanagi H,Ozawa K,Kadoi H,Okochi M,Matsunaga T

更新日期：2006-10-20 00:00:00

abstract：:In this short review, we describe the use of high molecular weight proteins produced in the glands of several arthropods-commonly called silks-for the purpose to enhance human skin wound healing. To this end an extensive literature search has been performed, the publications have been categorized concerning silk prepa...

journal_title：Biotechnology and bioengineering

authors： Reimers K,Liebsch C,Radtke C,Kuhbier JW,Vogt PM

更新日期：2015-11-01 00:00:00

abstract：:Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...

journal_title：Biotechnology and bioengineering

authors： Ren H,Hu P,Zhao H

更新日期：2017-08-01 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:Peroxidase-catalyzed asymmetric sulfoxidations, while synthetically attractive, suffer from relatively low reaction rates due to poor substrate solubilities in water and from appreciable spontaneous oxidation of substrates (especially aryl alkyl sulfides) with H(2)O(2). In this work, we found that both of these shortc...

journal_title：Biotechnology and bioengineering

authors： Dai L,Klibanov AM

更新日期：2000-11-05 00:00:00

abstract：:The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...

journal_title：Biotechnology and bioengineering

authors： Kunert R,Steinfellner W,Purtscher M,Assadian A,Katinger H

更新日期：2000-01-05 00:00:00

abstract：:The yeast Pachysolen tannophilus was entrapped in calcium alginate beads to ferment D-xylose on a continous basis in the presence of high cell densities. Experimental operating variables included the feed D-xylose concentration, the dilution rate, and the fermentor biomass concentration. Under favorable operating cond...

journal_title：Biotechnology and bioengineering

authors： Slininger PJ,Bothast RJ,Black LT,McGhee JE

更新日期：1982-10-01 00:00:00

abstract：:A two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. The first bioreactor was employed for cell growth and the second for cell infection. Silkworm Bm5 cells were infected with a recombinant baculovirus, BmNPV/P...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Kalogerakis N,Behie LA,Iatrou K

更新日期：1993-07-01 00:00:00

abstract：:A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...

journal_title：Biotechnology and bioengineering

authors： Pathi B,Kinsey ST,Locke BR

更新日期：2011-08-01 00:00:00

abstract：:In addition to the role of maintaining the pH, buffers can also facilitate the transport of H(+) ions in acid-generating systems. The role of this facilitation in proton transport in porous pellets on acid-generating immobilized enzymic reactions is examined. The activity in these systems can be maximized by a proper ...

journal_title：Biotechnology and bioengineering

authors： Ruckenstein E,Rajora P

更新日期：1985-06-01 00:00:00

abstract：:The ability of algae and bacteria to accumulate heavy metals from the surrounding environment is a widely recognized phenomenon that has a number of important implications. This work reports on the development of a quantitative model that addresses the basic mechanisms inherent in many uptake processes. The model post...

journal_title：Biotechnology and bioengineering

authors： Ting YP,Lawson F,Prince IG

更新日期：1989-10-05 00:00:00