Abstract:
:Recently, the respiratory quotient (RQ) of microbes measured in situ in a fermentor by exit-gas analysis has been used successfully, for instance, in a fed-batch culture of baker's yeast as a criterion to control the feeding rate.(1-3) It is significant here to keep RQ values close to unity throughout; any deviations of RQ from unity give rise to deterioration of the cell growth yield.However easy it might be to keep RQ values around unity by controlling the feeding rate, the question of whether or not RQ values determined by gas analysis at the fermentor exit could generally represent those in vivo deserves attention. Indeed, for a fermentation carried out at an alkaline side, gas analysis would give RQ values that differ remarkably from true values because of the medium's "storage" of CO(2) released from microbes. The purpose of this communication is to make clear those factors that would affect true RQ values in the analysis of exit gas from a fermentor.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Aiba S,Furuse Hdoi
10.1002/bit.260360513subject
Has Abstractpub_date
1990-08-20 00:00:00pages
534-8issue
5eissn
0006-3592issn
1097-0290journal_volume
36pub_type
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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