Production of S-adenosyl-L-methionine by Saccharomyces cerevisiae cells carrying a gene for ethionine resistance.

abstract：:Human tissue-type plasminogen activator (t-PA) contains a variably occupied glycosylation site at Asn-184 in naturally produced t-PA and in t-PA produced in recombinant Chinese hamster ovary (CHO) cells. The presence of an oligosaccharide at this site has previously been shown to reduce specific activity and fibrin bi...

journal_title：Biotechnology and bioengineering

authors： Andersen DC,Bridges T,Gawlitzek M,Hoy C

更新日期：2000-10-05 00:00:00

abstract：:A fuzzy-logic light attenuation model was developed and validated for a stirred-tank photobioreactor. Based on this model, local light intensities were used to calculate local specific growth rates of the cyanobacteria Synechococcus PCC 7942. The light regime for maximization of biomass space-time yield in a batch pro...

journal_title：Biotechnology and bioengineering

authors： Franco-Lara E,Havel J,Peterat F,Weuster-Botz D

更新日期：2006-12-20 00:00:00

abstract：:Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and gro...

journal_title：Biotechnology and bioengineering

authors： Domingues L,Lima N,Teixeira JA

更新日期：2001-03-05 00:00:00

abstract：:This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fa...

journal_title：Biotechnology and bioengineering

authors： Velander WH,Subramanian A,Madurawe RD,Orthner CL

更新日期：1992-04-25 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...

journal_title：Biotechnology and bioengineering

authors： James RI,Elton JP,Todd P,Kompala DS

更新日期：2000-01-20 00:00:00

abstract：:Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

journal_title：Biotechnology and bioengineering

authors： Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

更新日期：2006-02-20 00:00:00

abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...

journal_title：Biotechnology and bioengineering

authors： Bryers JD

更新日期：1985-05-01 00:00:00

abstract：:The bioconversion of octanoic acid into 2-heptanone by spores of Penicillium roquefortii is performed using a fed-batch technique with pH control by addition of the liquid substrate itself. The early stage of this process takes place with a high bioconversion rate and high yield. These values then decrease as a result...

journal_title：Biotechnology and bioengineering

authors： Larroche C,Besson I,Gros JB

更新日期：1994-09-05 00:00:00

abstract：:Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...

journal_title：Biotechnology and bioengineering

authors： Hou XD,Li N,Zong MH

更新日期：2013-07-01 00:00:00

abstract：:Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Five) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire information relevant to la...

journal_title：Biotechnology and bioengineering

authors： Rhiel M,Mitchell-Logean CM,Murhammer DW

更新日期：1997-09-20 00:00:00

abstract：:Bioprocesses operated in batch mode can induce adverse effects like overflow metabolism, substrate inhibition, osmotic inhibition, oxygen limitation, and catabolite repression. To avoid these adverse effects, fed-batch is the predominant operation mode in industrial production. Nevertheless, screening for optimal prod...

journal_title：Biotechnology and bioengineering

authors： Habicher T,Czotscher V,Klein T,Daub A,Keil T,Büchs J

更新日期：2019-09-01 00:00:00

abstract：:Strains from Trichoderma reesei have been used for cellulase production with a long history. It has been well known that cellulase biosynthesis by the fungal species is controlled through regulators, and elucidation of their regulation network is of great importance for engineering T. reesei with robust cellulase prod...

journal_title：Biotechnology and bioengineering

authors： Meng QS,Zhang F,Liu CG,Zhao XQ,Bai FW

更新日期：2020-06-01 00:00:00

abstract：:The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially incr...

journal_title：Biotechnology and bioengineering

authors： Jones EO,Lee JM

更新日期：1988-01-01 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in pr...

journal_title：Biotechnology and bioengineering

authors： Wu FJ,Friend JR,Lazar A,Mann HJ,Remmel RP,Cerra FB,Hu WS

更新日期：1996-10-05 00:00:00

abstract：:The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponen...

journal_title：Biotechnology and bioengineering

authors： Zheng ZM,Xu YZ,Liu HJ,Guo NN,Cai ZZ,Liu DH

更新日期：2008-08-01 00:00:00

abstract：:In this report, alpha-Amylase originating from Bacillus subtilis (liquefying type) was immobilized on partially imidoesterized polyacrylonitrile (PAN) by covalent bonding. For the preparation of immobilized alpha-amylase, which has a high activity and high stability to repeated use, the optimum conditions for the prep...

journal_title：Biotechnology and bioengineering

authors： Handa T,Hirose A,Akino T,Watanabe K,Tsuchiya H

更新日期：1983-12-01 00:00:00

abstract：:The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...

journal_title：Biotechnology and bioengineering

authors： Fox SR,Patel UA,Yap MG,Wang DI

更新日期：2004-01-20 00:00:00

abstract：:Incremental truncation is a method for constructing libraries of every one base pair truncation of a segment of DNA. Incremental truncation libraries can be created using a time-dependent nuclease method or through the incorporation of alpha-phosphothioate dNTPs by PCR or by primer extension (THIO(pcr) truncation and ...

journal_title：Biotechnology and bioengineering

authors： Ostermeier M

更新日期：2003-06-05 00:00:00

abstract：:Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature profiles were fairly similar up to 55 degrees C. However, above this temper...

journal_title：Biotechnology and bioengineering

authors： Dickensheets PA,Chen LF,Tsao GT

更新日期：1977-03-01 00:00:00

abstract：:Air emissions from the pulp and paper industry frequently contain reduced sulfur compounds (RSC), such as dimethyl sulfide (DMS) mixed with volatile organic compounds (VOC) (e.g., methanol, MeOH) and it is desirable to treat either one or both of these groups of compounds. The objective of this study was to assess the...

journal_title：Biotechnology and bioengineering

authors： Zhang Y,Liss SN,Allen DG

更新日期：2006-11-05 00:00:00

abstract：:The effector functions elicited by IgG antibodies strongly depend on the carbohydrate moiety linked to the Fc region of the protein. Therefore several approaches have been developed to rationally manipulate these glycans and improve the biological functions of the antibody. Overexpression of recombinant beta1,4-N-acet...

journal_title：Biotechnology and bioengineering

authors： Ferrara C,Brünker P,Suter T,Moser S,Püntener U,Umaña P

更新日期：2006-04-05 00:00:00

abstract：:A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a pr...

journal_title：Biotechnology and bioengineering

authors： Nie H,Soh BW,Fu YC,Wang CH

更新日期：2008-01-01 00:00:00

abstract：:Biofiltration of air polluted by volatile organic compounds is now recognized by the industrial and research communities as an effective and viable alternative to standard environmental technologies. Whereas many studies have focused on solid/liquid/gas biofilters, there have been fewer reports on waste air treatment ...

journal_title：Biotechnology and bioengineering

authors： Erable B,Goubet I,Lamare S,Legoy MD,Maugard T

更新日期：2004-04-05 00:00:00

abstract：:Both k(L)a and k(L) measurements were carried out by an unsteady state technique at impeller speeds ranging from 1.6 to 5.8 s(-1) in a mechanically agitated animal cell culture vessel of working volume 1.5 L. Checks were made that the time constant of the oxygen electrode was negligible compared to the time for aerati...

journal_title：Biotechnology and bioengineering

authors： Lavery M,Nienow AW

更新日期：1987-08-20 00:00:00

abstract：:The influence of biofilm areal cell density, species composition, and the presence of abiotic particles on the disinfection and removal of bacterial biofilms by monochloramine was investigated. Mono- and binary population biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown on stainless-steel slides...

journal_title：Biotechnology and bioengineering

authors： Srinivasan R,Stewart PS,Griebe T,Chen CI,Xu X

更新日期：1995-06-20 00:00:00

abstract：:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

journal_title：Biotechnology and bioengineering

authors： Schinkel H,Jacobs P,Schillberg S,Wehner M

更新日期：2008-01-01 00:00:00

abstract：:Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

journal_title：Biotechnology and bioengineering

authors： Braud A,Jézéquel K,Léger MA,Lebeau T

更新日期：2006-08-20 00:00:00

abstract：:We consider the application of high-throughput experimentation (HTE), including combinatorial methods, to catalyst discovery and early-phase optimization. While combinatorial- and parallel-testing methods have an already substantial history in catalysis, recent work by several groups promises significant efficiency ga...

journal_title：Biotechnology and bioengineering

authors： Newsam JM,Schüth F

更新日期：1998-01-01 00:00:00

abstract：:The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed micelles of dioctyl sodium sulfosuccinate (AOT) in isooctane was investigated in an ultrafiltration ceramic membrane reactor of tubular type, operating in a batch mode. Water concentration was found to be a critical ...

journal_title：Biotechnology and bioengineering

authors： Prazers DM,Garcia FA,Cabral JM

更新日期：1993-04-05 00:00:00