Three-dimensional fibrous PLGA/HAp composite scaffold for BMP-2 delivery.


:A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a promising fabrication technique, electrospinning. In vitro release of BMP-2 from these scaffolds, and the attachment ability and viability of marrow derived messenchymal stem cells (MSCs) in the presence of the scaffolds were investigated. The PLGA/HAp composite scaffolds developed in this study exhibit good morphology and it was observed that HAp nanoparticles were homogeneously dispersed inside PLGA matrix within the scaffold. The composite scaffolds allowed sustained (2-8 weeks) release of BMP-2 whose release rate was accelerated with increasing HAp content. It was also shown that BMP-2 protein successfully maintained its integrity and natural conformations after undergoing the process of electrospinning. Cell culture experiments showed that the encapsulation of HAp could enhance cell attachment to scaffolds and lower cytotoxicity.


Biotechnol Bioeng


Nie H,Soh BW,Fu YC,Wang CH




Has Abstract


2008-01-01 00:00:00












  • Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery.

    abstract::The twin arginine translocation (Tat) pathway occurs naturally in E. coli and has the distinct ability to translocate folded proteins across the inner membrane of the cell. It has the potential to export commercially useful proteins that cannot be exported by the ubiquitous Sec pathway. To better understand the biopro...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Branston SD,Matos CF,Freedman RB,Robinson C,Keshavarz-Moore E

    更新日期:2012-04-01 00:00:00

  • Immobilization of yeast cells containing beta-galactosidase.

    abstract::Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-ga...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Banerjee M,Chakrabarty A,Majumdar SK

    更新日期:1982-08-01 00:00:00

  • Progression of continuous downstream processing of monoclonal antibodies: Current trends and challenges.

    abstract::Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Somasundaram B,Pleitt K,Shave E,Baker K,Lua LHL

    更新日期:2018-12-01 00:00:00

  • Dilution rate as a determinant of mycelial morphology in continuous culture.

    abstract::The morphology of mycelial fungi in liquid culture effects culture rheology and this in turn may affect product yield. It is therefore important to understand how environmental factors influence mycelial morphology and this paper describes the effect of dilution rate on two strains of Fusarium graminearum, the relativ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wiebe MG,Trinci AP

    更新日期:1991-06-05 00:00:00

  • Metabolic engineering of Ralstonia eutropha for the production of polyhydroxyalkanoates from sucrose.

    abstract::A sucrose utilization pathway was established in Ralstonia eutropha NCIMB11599 and R. eutropha 437-540 by introducing the Mannheimia succiniciproducens MBEL55E sacC gene that encodes β-fructofuranosidase. These engineered strains were examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park SJ,Jang YA,Noh W,Oh YH,Lee H,David Y,Baylon MG,Shin J,Yang JE,Choi SY,Lee SH,Lee SY

    更新日期:2015-03-01 00:00:00

  • Production of Bacillus thuringiensis spores in total cell retention culture and two-stage continuous culture using an internal ceramic filter system.

    abstract::The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial ce...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kang BC,Lee SY,Chang HN

    更新日期:1993-11-05 00:00:00

  • "Enzyme Test Bench," a high-throughput enzyme characterization technique including the long-term stability.

    abstract::A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rachinskiy K,Schultze H,Boy M,Bornscheuer U,Büchs J

    更新日期:2009-06-01 00:00:00

  • Engineering the pentose phosphate pathway to improve hydrogen yield in recombinant Escherichia coli.

    abstract::Among various routes for the biological hydrogen production, the NAD(P)H-dependent pentose phosphate (PP) pathway is the most efficient for the dark fermentation. Few studies, however, have focused on the glucose-6-phosphate 1-dehydrogenase, encoded by zwf, as a key enzyme activating the PP pathway. Although the gluco...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim YM,Cho HS,Jung GY,Park JM

    更新日期:2011-12-01 00:00:00

  • A mass balance study to assess the extent of contaminant removal achieved in the operations for the primary recovery of plasmid DNA from Escherichia coli cells.

    abstract::Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ciccolini LA,Shamlou PA,Titchener-Hooker N

    更新日期:2002-03-30 00:00:00

  • Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet.

    abstract::Modification of cellular functions by overexpression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ino K,Kawasumi T,Ito A,Honda H

    更新日期:2008-05-01 00:00:00

  • Application of a mechanistic model as a tool for on-line monitoring of pilot scale filamentous fungal fermentation processes-The importance of evaporation effects.

    abstract::A mechanistic model-based soft sensor is developed and validated for 550L filamentous fungus fermentations operated at Novozymes A/S. The soft sensor is comprised of a parameter estimation block based on a stoichiometric balance, coupled to a dynamic process model. The on-line parameter estimation block models the cha...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mears L,Stocks SM,Albaek MO,Sin G,Gernaey KV

    更新日期:2017-03-01 00:00:00

  • Determination of cells' water membrane permeability: unexpected high osmotic permeability of Saccharomyces cerevisiae.

    abstract::Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: de Marañón IM,Gervais P,Molin P

    更新日期:1997-10-05 00:00:00

  • Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors.

    abstract::Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

    更新日期:2017-10-01 00:00:00

  • Engineering multigene expression in vitro and in vivo with small terminators for T7 RNA polymerase.

    abstract::Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Du L,Gao R,Forster AC

    更新日期:2009-12-15 00:00:00

  • Effects of culture conditions on N-glycolylneuraminic acid (Neu5Gc) content of a recombinant fusion protein produced in CHO cells.

    abstract::CHO cells express glycoproteins containing both the N-acetylneuraminic acid (Neu5Ac) and minor amounts of the N-glycolylneuraminic acid (Neu5Gc) forms of sialic acid. As Neu5Gc is not expressed in humans and can be recognized as a foreign epitope, there is the potential for immunogenicity issues for glycoprotein thera...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Borys MC,Dalal NG,Abu-Absi NR,Khattak SF,Jing Y,Xing Z,Li ZJ

    更新日期:2010-04-15 00:00:00

  • Increased curvature of hollow fiber membranes could up-regulate differential functions of renal tubular cell layers.

    abstract::Tissue engineering devices as in vitro cell culture systems in scaffolds has encountered the bottleneck due to their much lower cell functions than real tissues/organs in vivo. Such situation has been improved in some extent by mimicking the cell microenvironments in vivo from either chemical or physical ways. However...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shen C,Meng Q,Zhang G

    更新日期:2013-08-01 00:00:00

  • Optimization of recombinant protein expression level in Escherichia coli by flow cytometry and cell sorting.

    abstract::Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Soriano E,Borth N,Katinger H,Mattanovich D

    更新日期:2002-10-05 00:00:00

  • Measuring kLa with randomly pulsed dynamic method.

    abstract::This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo-random bina...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gauthier L,Thibault J,LeDuy A

    更新日期:1991-04-15 00:00:00

  • Application of continuous zone electrophoresis to preparative separation of proteins.

    abstract::A comprehensive study of the application of continuous zone electrophoresis to preparative separation of proteins in free solution is presented. First, the influence of electric field strength, buffer residence time in the chamber, sample flow rate, and sample concentration on separation resolution and throughput were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nath S,Schütte H,Hustedt H,Deckwer WD

    更新日期:1993-09-20 00:00:00

  • Optimal production of glutathione by controlling the specific growth rate of yeast in fed-batch culture.

    abstract::The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shimizu H,Araki K,Shioya S,Suga K

    更新日期:1991-06-20 00:00:00

  • Chemically stabilized trypsin used in dipeptide synthesis.

    abstract::Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Murphy A,O Fágáin C

    更新日期:1998-05-20 00:00:00

  • Extension of cell membrane boosting squalene production in the engineered Escherichia coli.

    abstract::Squalene is a lipophilic and non-volatile triterpene with many industrial applications for food, pharmaceuticals, and cosmetics. Metabolic engineering focused on optimization of the production pathway suffer from little success in improving titers because of a limited space of the cell membrane accommodating the lipop...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Meng Y,Shao X,Wang Y,Li Y,Zheng X,Wei G,Kim SW,Wang C

    更新日期:2020-07-21 00:00:00

  • Large-scale gene expression analysis of cholesterol dependence in NS0 cells.

    abstract::NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Seth G,Philp RJ,Denoya CD,McGrath K,Stutzman-Engwall KJ,Yap M,Hu WS

    更新日期:2005-06-05 00:00:00

  • Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity.

    abstract::The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient ae...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Thomas B,Ohde D,Matthes S,Engelmann C,Bubenheim P,Terasaka K,Schlüter M,Liese A

    更新日期:2021-01-01 00:00:00

  • Production of savinase and population viability of Bacillus clausii during high-cell-density fed-batch cultivations.

    abstract::The growth and product formation of a Savinase-producing Bacillus clausii were investigated in high-cell-density fed-batch cultivations with both linear and exponential feed profiles. The highest specific productivity of Savinase was observed shortly after the end of the initial batch phase for all feed profiles appli...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Christiansen T,Michaelsen S,Wümpelmann M,Nielsen J

    更新日期:2003-08-05 00:00:00

  • Tetracycline-regulated overexpression of glycosyltransferases in Chinese hamster ovary cells.

    abstract::The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. Here we use tetracyc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Umaña P,Jean-Mairet J,Bailey JE

    更新日期:1999-12-05 00:00:00

  • Identification of manipulated variables for a glycosylation control strategy.

    abstract::N-linked glycan distribution affects important end-use characteristics such as the bioactivity and efficacy of many therapeutic proteins, (including monoclonal antibodies), in vivo. Yet, obtaining desired glycan distributions consistently during batch-to-batch production can be challenging for biopharmaceutical manufa...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: St Amand MM,Radhakrishnan D,Robinson AS,Ogunnaike BA

    更新日期:2014-10-01 00:00:00

  • Reaction characteristics and stability of a membrane-bound enzyme reconstituted in bilayers of liposomes.

    abstract::The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kheirolomoom A,Katoh S,Sada E,Yoshida K

    更新日期:1991-04-15 00:00:00

  • Ethanol utilization by sulfate-reducing bacteria: an experimental and modeling study.

    abstract::A mixed culture of sulfate-reducing bacteria containing the species Desulfovibrio desulfuricans was used to study sulfate-reduction stoichiometry and kinetics using ethanol as the carbon source. Growth yield was lower, and kinetics were slower, for ethanol compared to lactate. Ethanol was converted into acetate and no...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nagpal S,Chuichulcherm S,Livingston A,Peeva L

    更新日期:2000-12-05 00:00:00

  • The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes.

    abstract::The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glut...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Miller WM,Wilke CR,Blanch HW

    更新日期:1989-01-20 00:00:00