Mutant Escherichia coli penicillin acylase with enhanced stability at alkaline pH.

abstract：:The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradation of PAHs in soils can be enhanced by employing surface-active agents. However, chemical surfactants are often recalcitrant and exer...

journal_title：Biotechnology and bioengineering

authors： Fava F,Berselli S,Conte P,Piccolo A,Marchetti L

更新日期：2004-10-20 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...

journal_title：Biotechnology and bioengineering

authors： Feins M,Sirkar KK

更新日期：2004-06-20 00:00:00

abstract：:In macroscopic dynamic models of fermentation processes, elementary modes (EM) derived from metabolic networks are often used to describe the reaction stoichiometry in a simplified manner and to build predictive models by parameterizing kinetic rate equations for the EM. In this procedure, the selection of a set of EM...

journal_title：Biotechnology and bioengineering

authors： Hebing L,Neymann T,Engell S

更新日期：2020-07-01 00:00:00

abstract：:The extracellular matrix provides structural components that support the development of tissue morphology and the distribution of growth factors that modulate the overall cellular response to those growth factors. The ability to manipulate the presentation of factors in culture systems should provide an additional deg...

journal_title：Biotechnology and bioengineering

authors： Jervis EJ,Guarna MM,Doheny JG,Haynes CA,Kilburn DG

更新日期：2005-08-05 00:00:00

abstract：:Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...

journal_title：Biotechnology and bioengineering

authors： Zhou Y,Suenaga T,Qi C,Riya S,Hosomi M,Terada A

更新日期：2020-12-11 00:00:00

abstract：:In eukaryotes, secretory proteins are folded and assembled in the endoplasmic reticulum (ER). Many heterologous proteins are retained in the ER due to suboptimal folding conditions. We previously reported that heterologous secretion of Pyrococcus furiosus beta-glucosidase in Saccharomyces cerevisiae resulted in the ac...

journal_title：Biotechnology and bioengineering

authors： Smith JD,Tang BC,Robinson AS

更新日期：2004-02-05 00:00:00

abstract：:A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

journal_title：Biotechnology and bioengineering

authors： Goey CH,Tsang JMH,Bell D,Kontoravdi C

更新日期：2017-12-01 00:00:00

abstract：:A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifyi...

journal_title：Biotechnology and bioengineering

authors： Terada A,Yamamoto T,Tsuneda S,Hirata A

更新日期：2006-07-05 00:00:00

abstract：:A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow-fiber ultrafiltration technology under conditions of large-scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5-fo...

journal_title：Biotechnology and bioengineering

authors： Weiss SA

更新日期：1980-01-01 00:00:00

abstract：:Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(e...

journal_title：Biotechnology and bioengineering

authors： Racine J,Cheradame H,Chu YS,Thiery JP,Rodriguez I

更新日期：2011-04-01 00:00:00

abstract：:L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose has been achieved in Escherichia coli. However, there are still several limiting factors hindering further improvement of l-threonine productivity...

journal_title：Biotechnology and bioengineering

authors： Liu J,Li H,Xiong H,Xie X,Chen N,Zhao G,Caiyin Q,Zhu H,Qiao J

更新日期：2019-01-01 00:00:00

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:Although sophorolipids (SLs) produced by S. bombicola are a real showcase for the industrialization of microbial biosurfactants, some important drawbacks are associated with this efficient biological process, e.g., the simultaneous production of acidic and lactonic SLs. Depending on the application, there is a require...

journal_title：Biotechnology and bioengineering

authors： Roelants SL,Ciesielska K,De Maeseneire SL,Moens H,Everaert B,Verweire S,Denon Q,Vanlerberghe B,Van Bogaert IN,Van der Meeren P,Devreese B,Soetaert W

更新日期：2016-03-01 00:00:00

abstract：:Many bioprocesses depend on the effective formation of a biofilm on a solid support. In the present study, three different surface treatments (sandblasting, pure-O(2) plasma, and He-O(2) plasma treatments) were conducted on polypropylene (PP) Pall rings used as a support in biotrickling filters for air pollution contr...

journal_title：Biotechnology and bioengineering

authors： Prado OJ,Popat SC,Chen G,Walker SL,Lafuente J,Gabriel D,Deshusses MA

更新日期：2009-08-15 00:00:00

abstract：:Cellulosomes are large plant cell wall degrading complexes secreted by some anaerobic bacteria. They are typically composed of a major scaffolding protein containing multiple receptors called cohesins, which tightly anchor a small complementary module termed dockerin harbored by the cellulosomal enzymes. In the presen...

journal_title：Biotechnology and bioengineering

authors： Vita N,Borne R,Fierobe HP

更新日期：2020-03-01 00:00:00

abstract：:Metabolic engineering of isopropyl alcohol (IPA)-producing Escherichia coli strains was conducted along with 13 C-metabolic flux analysis (MFA). A metabolically engineered E. coli strain expressing the adc gene derived from Clostridium acetobutylicum and the IPADH gene from C. beijerinckii did not produce IPA during i...

journal_title：Biotechnology and bioengineering

authors： Okahashi N,Matsuda F,Yoshikawa K,Shirai T,Matsumoto Y,Wada M,Shimizu H

更新日期：2017-12-01 00:00:00

abstract：:The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial ce...

journal_title：Biotechnology and bioengineering

authors： Kang BC,Lee SY,Chang HN

更新日期：1993-11-05 00:00:00

abstract：:The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glut...

journal_title：Biotechnology and bioengineering

authors： Miller WM,Wilke CR,Blanch HW

更新日期：1989-01-20 00:00:00

abstract：:A two-dimensional model for quantitative evaluation of the effect of convective and diffusive substrate transport on biofilm heterogeneity was developed. The model includes flow computation around the irregular biofilm surface, substrate mass transfer by convection and diffusion, biomass growth, and biomass spreading....

journal_title：Biotechnology and bioengineering

authors： Picioreanu C,Van Loosdrecht MC,Heijnen JJ

更新日期：2000-09-05 00:00:00

abstract：:Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-ga...

journal_title：Biotechnology and bioengineering

authors： Banerjee M,Chakrabarty A,Majumdar SK

更新日期：1982-08-01 00:00:00

abstract：:Continuous propionic acid fermentations of lactate by Propionibacterium acidipropionici were studied in spiral wound fibrous bed bioreactors. Cells were imobilized by natural attachment to fiber surfaces and entrapment in the void volume within the fibrous matrix. A high cell density of approximately 37 g/L was attain...

journal_title：Biotechnology and bioengineering

authors： Lewis VP,Yang ST

更新日期：1992-08-05 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:Oxygen and nutrient limitation was investigated in order to identify the origin of a lower specific ajmalicine production in Catharanthus roseus cultures at high cell densities in an induction medium. The effect of oxygen limitation was explored by comparing two identically aerated and agitated high cell density biore...

journal_title：Biotechnology and bioengineering

authors： Schlatmann JE,Moreno PR,Vinke JL,Ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1994-08-05 00:00:00

abstract：:The patterning of cardiac myocytes on a micron scale ( approximately 5 microm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca(2+) transition o...

journal_title：Biotechnology and bioengineering

authors： Kaji H,Takoh K,Nishizawa M,Matsue T

更新日期：2003-03-20 00:00:00

abstract：:This work investigated the formation of microbial granules to boost the productivity of lactic acid (LA). The flocculated form of LA-producing microbial consortium, dominated by Lactobacillus sp. (91.5% of total sequence), was initially obtained in a continuous stirred-tank reactor (CSTR), which was fed with 2% glucos...

journal_title：Biotechnology and bioengineering

authors： Kim DH,Lee MK,Hwang Y,Im WT,Yun YM,Park C,Kim MS

更新日期：2016-01-01 00:00:00

abstract：:This study demonstrated that hydrogen-producing acidogenic sludge could agglutinate into granules in a well-mixed reactor treating a synthetic sucrose-containing wastewater at 26 degrees C, pH 5.5, with 6 h of hydraulic retention. A typical matured granule is 1.6 mm in diameter, 1.038 g/mL in density, 11% in ash conte...

journal_title：Biotechnology and bioengineering

authors： Fang HH,Liu H,Zhang T

更新日期：2002-04-05 00:00:00

abstract：:Two simultaneous fermentations were performed at 26 degrees C with simultaneous inocula using Clostridium saccharoperbutylacetonicum. Fermentation 1 prevented the gas formed by the biomass from escaping the fermentor while 2 allowed the gas formed to escape. Fermentor 1 provided for the production of butanol, acetone,...

journal_title：Biotechnology and bioengineering

authors： Brosseau JD,Yan JY,Lo KV

更新日期：1986-03-01 00:00:00

abstract：:Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m(3) occurred at pH 4.0 and a dilution rate of 0.0391 h(-1) where residual substrate was 0.43 kg/m(3), but no cellulase wa...

journal_title：Biotechnology and bioengineering

authors： Schafner DW,Toledo RT

更新日期：1992-04-05 00:00:00

abstract：:Currently in America the biofuel ethanol is primarily being produced by the dry grind technique to obtain the starch contained in the corn grains and subsequently subjected to fermentation. This so-called 1st generation technology has two setbacks; first the lingering debate whether its life cycle contributes to a red...

journal_title：Biotechnology and bioengineering

authors： Brehmer B,Bals B,Sanders J,Dale B

更新日期：2008-09-01 00:00:00