The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes.

Abstract:

:The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glutamine additions, but the responses of the glucose and oxygen consumption rates and the cell concentration were found to depend on the intial feed glutamine concentration. The glucose consumption rate was 1.4-10.9 times that of glutamine, and serine and branched-chain amino acids were consumed in larger amounts at the higher glucose: glutamine uptake ratios. It was estimated that maintenance accounted for ca. 60% of the cellular ATP requirements at specific growth rates ranging from 0.57 to 0.68 day(-1).

journal_name

Biotechnol Bioeng

authors

Miller WM,Wilke CR,Blanch HW

doi

10.1002/bit.260330414

subject

Has Abstract

pub_date

1989-01-20 00:00:00

pages

487-99

issue

4

eissn

0006-3592

issn

1097-0290

journal_volume

33

pub_type

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