The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes.


:The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glutamine additions, but the responses of the glucose and oxygen consumption rates and the cell concentration were found to depend on the intial feed glutamine concentration. The glucose consumption rate was 1.4-10.9 times that of glutamine, and serine and branched-chain amino acids were consumed in larger amounts at the higher glucose: glutamine uptake ratios. It was estimated that maintenance accounted for ca. 60% of the cellular ATP requirements at specific growth rates ranging from 0.57 to 0.68 day(-1).


Biotechnol Bioeng


Miller WM,Wilke CR,Blanch HW




Has Abstract


1989-01-20 00:00:00












  • Fractional precipitation of plasmid DNA from lysate by CTAB.

    abstract::Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lander RJ,Winters MA,Meacle FJ,Buckland BC,Lee AL

    更新日期:2002-09-30 00:00:00

  • Optimization of microalgal production in a shallow outdoor flume.

    abstract::The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Laws EA,Taguchi S,Hirata J,Pang L

    更新日期:1988-07-05 00:00:00

  • Salt-induced changes in plasmid DNA transmission through ultrafiltration membranes.

    abstract::Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Latulippe DR,Zydney AL

    更新日期:2008-02-01 00:00:00

  • Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization.

    abstract::A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang M,Yu C,Zhao H

    更新日期:2016-03-01 00:00:00

  • Biotransformation of D-methionine into L-methionine in the cascade of four enzymes.

    abstract::D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Findrik Z,Vasić-Racki D

    更新日期:2007-12-01 00:00:00

  • Image analysis to quantify and measure UASB digester granules.

    abstract::Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

    更新日期:1993-07-01 00:00:00

  • A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus.

    abstract::The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gilbert PA,Kamen A,Bernier A,Garnier A

    更新日期:2007-09-01 00:00:00

  • Kinetic resolution of racemic glycidyl butyrate using a multiphase membrane enzyme reactor: experiments and model verification.

    abstract::A laboratory-scale multiphase hollow fiber membrane reactor was employed to investigate the lipase-catalyzed enzymatic resolution of racemic glycidyl butyrate. A mathematical formulation was developed to simulate the performance of this system. Model parameters were determined independently (except the effective rate ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wu DR,Cramer SM,Belfort G

    更新日期:1993-04-25 00:00:00

  • Influence of power supply in the feasibility of Phaeodactylum tricornutum cultures.

    abstract::The influence of fluid-dynamic conditions on the yield of Phaeodactylum tricornutum microalgal cultures was analyzed in two stages: first, the influence of air flow rate; second, the influence of using fluid-moving pumps for recirculating the culture. With respect to the air flow rate, the yield of the cultures increa...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Alías CB,García-Malea López MC,Acién Fernández FG,Ferníndez Sevilla JM,García Sánchez JL,Molina Grima E

    更新日期:2004-09-20 00:00:00

  • Mechanized systems for media dispensing, inoculation, and replication of microorganisms.

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Stieglitz B,Defelice CP

    更新日期:1986-09-01 00:00:00

  • Cultivation of hybridoma cells in an inclined bioreactor.

    abstract::Murine hybridoma cells were grown in a bubble column that was inclined up to 45 degrees from vertical. Inclining the column by a few degrees separated the rising bubbles against the upper surface, leaving the bulk of the liquid bubble free. The liquid was circulated well by the rising bubbles, but collection of cells ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lu GZ,Gray MR,Thompson BG,Suresh MR

    更新日期:1995-01-20 00:00:00

  • Development of waxy cassava with different Biological and physico-chemical characteristics of starches for industrial applications.

    abstract::The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

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  • Dynamic pressure method for kla measurement in large-scale bioreactors.

    abstract::A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Linek V,Benes P,Vacek V

    更新日期:1989-05-01 00:00:00

  • Mutant Escherichia coli penicillin acylase with enhanced stability at alkaline pH.

    abstract::Increased stability at alkaline pH should be a valuable attribute for the utilization of penicillin acylase in bioreactors employed to convert penicillins into 6-aminopenicillanic acid, a precursor of semisynthetic penicillins. In these systems, base is added for pH control, which results in local alkaline conditions ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Del Río G,Rodríguez ME,Munguía ME,López-Munguí A,Soberón X

    更新日期:1995-10-20 00:00:00

  • Kinetics of pure cultures of hydrogen-oxidizing denitrifying bacteria and modeling of the interactions among them in mixed cultures.

    abstract::In this study we report the isolation of four denitrifying bacteria from a batch reactor, where the progress of hydrogenotrophic denitrification was examined. Only three of the strains had the ability to use hydrogen as electron donor. In the present work, kinetic batch experiments were carried out in order to study t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vasiliadou IA,Siozios S,Papadas IT,Bourtzis K,Pavlou S,Vayenas DV

    更新日期:2006-10-20 00:00:00

  • Continuous-culture studies of synthesis and regulation of extracellular beta(1-3) glucanase and protease enzymes from Oerskovia xanthineolytica.

    abstract::This article describes the synthesis and regulation of beta(1-3)glucanase and protease enzymes from the cell lytic system of Oerskovia xanthineolytica LL-G109 in continuous culture using different concentrations of carbon source (glucose) and inducer (glucan). These two enzyme activities are the main components of a l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Andrews BA,Asenjo JA

    更新日期:1987-10-05 00:00:00

  • Immobilization of Streptomyces phaerochromogenes by radiation-induced polymerization of glass-forming monomers.

    abstract::Immobilization of Streptomyces phaerochromogenes was studied by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures. Radiation damage of the enzyme could be avoided by choosing irradiation at low temperatures. The enzymatic activity of immobilized cells increased remarkably with a decre...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kumakura M,Yoshida M,Kaetsu I

    更新日期:1979-04-01 00:00:00

  • Kinetics of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions.

    abstract::This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang QQ,Zhu JY,Hunt CG,Zhan HY

    更新日期:2012-08-01 00:00:00

  • Relationship between mass transfer coefficient and liquid flow velocity in heterogenous biofilms using microelectrodes and confocal microscopy.

    abstract::The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

    更新日期:1997-12-20 00:00:00

  • Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis.

    abstract::Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wiechert W,Siefke C,de Graaf AA,Marx A

    更新日期:1997-07-05 00:00:00

  • Expression of recombinant protein A from the lac promoter in Escherichia coli JM83 is not subject to catabolite repression when grown under specific conditions of continuous culture.

    abstract::Although widely used in experimental and industrial situations, genetically engineered plasmids containing the lac promoter from Escherichia coli are subject to catabolite repression when grown in glucose-containing media. Several methods of overcoming this problem have been investigated by studying the expression of ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Warnes A,Stephenson JR,Fooks AR,Melling J,Brown MR

    更新日期:1991-11-01 00:00:00

  • Mass transport parameters of aspen wood chip beds via stimulus-response tracer techniques.

    abstract::A stimulus-response tracer technique has been used to characterize packed beds of untreated, as well as acid prehydrolyzed, and enzymatically hydrolyzed aspen wood chips. Glucose was used as the tracer. Bulk liquid phase dispersion, interphase mass transfer, and intraparticle diffusion coefficients were determined for...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hradil G,Calo JM,Wunderlich TK Jr

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  • Stoichiometric analysis of animal cell growth and its application in medium design.

    abstract::Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was developed to study the stoichiometric demands for nutrients (including gluc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xie L,Wang DI

    更新日期:1994-05-01 00:00:00

  • A Kalman filter algorithm and monitoring apparatus for at-line control of fractional protein precipitation.

    abstract::Downstream processing operations are often carried out blind in the process timescale since product monitoring on-line is not common. Knowledge of the location and concentration of the product and key contaminants is complementary to other process information for process development and, if available on-line in conjun...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Holwill IJ,Chard SJ,Flanagan MT,Hoare M

    更新日期:1997-01-05 00:00:00

  • Development of liver microtissues with functional biliary ductular network.

    abstract::Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor's livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary d...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hafiz EOA,Bulutoglu B,Mansy SS,Chen Y,Abu-Taleb H,Soliman SAM,El-Hindawi AAF,Yarmush ML,Uygun BE

    更新日期:2021-01-01 00:00:00

  • Adsorption of cellulase from Trichoderma reesei on cellulose and lignacious residue in wood pretreated by dilute sulfuric acid with explosive decompression.

    abstract::The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ooshima H,Burns DS,Converse AO

    更新日期:1990-08-20 00:00:00

  • Utilization of light for the assimilation of organic matter in Chlorella sp. VJ79.

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lalucat J,Imperial J,Parés R

    更新日期:1984-07-01 00:00:00

  • Ethanol fermentation in a continuous tower fermentor.

    abstract::A mutant of Saccharomyces cerevisiae, which forms large, multicellular flocs in liquid culture, rapidly fermented media containing high concentrations of glucose (100-180 g/L) in a continuous nonaerated tower fermentor at 30 degrees C. The fermentor operated continuously for seven months. Batch and tower fermentor dat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jones ST,Korus RA,Admassu W,Heimsch RC

    更新日期:1984-07-01 00:00:00

  • Impact of membrane solid-liquid separation on design of biological nutrient removal activated sludge systems.

    abstract::Installing membranes for solid-liquid separation into biological nutrient removal (BNR) activated sludge (AS) systems makes a profound difference not only in the design of the BNR system itself, but also in the design approach for the whole wastewater treatment plant (WWTP). In multizone BNR systems with membranes in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ramphao M,Wentzel MC,Merritt R,Ekama GA,Young T,Buckley CA

    更新日期:2005-03-20 00:00:00

  • Effect of non-feeding period length on the intermittent operation of UASB reactors treating dairy effluents.

    abstract::Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Coelho NM,Rodrigues AA,Arroja LM,Capela IF

    更新日期:2007-02-01 00:00:00