Abstract:
:Embryonic stem cells (ESCs) are an important source of cardiomyocytes for regenerating injured myocardium. The successful use of ESC-derived cardiomyocytes in cardiac tissue engineering requires an understanding of the important scaffold properties and culture conditions to promote cell attachment, differentiation, organization, and contractile function. The goal of this work was to investigate how scaffold architecture and coculture with fibroblasts influences the differentiated phenotype of murine ESC-derived cardiomyocytes (mESCDCs). Electrospinning was used to process an elastomeric biodegradable polyurethane (PU) into aligned or unaligned fibrous scaffolds. Bioreactor produced mESCDCs were seeded onto the PU scaffolds either on their own or after pre-seeding the scaffolds with mouse embryonic fibroblasts (MEFs). Viable mESCDCs attached to the PU scaffolds and were functionally contractile in all conditions tested. Importantly, the aligned scaffolds led to the anisotropic organization of rod-shaped cells, improved sarcomere organization, and increased mESCDC aspect ratio (length-to-diameter ratio) when compared to cells on the unaligned scaffolds. In addition, pre-seeding the scaffolds with MEFs improved mESCDC sarcomere formation compared to mESCDCs cultured alone. These results suggest that both fiber alignment and pre-treatment of scaffolds with fibroblasts improve the differentiation of mESCDCs and are important parameters for developing engineered myocardial tissue constructs using ESC-derived cardiac cells.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Parrag IC,Zandstra PW,Woodhouse KAdoi
10.1002/bit.23353subject
Has Abstractpub_date
2012-03-01 00:00:00pages
813-22issue
3eissn
0006-3592issn
1097-0290journal_volume
109pub_type
杂志文章abstract::Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidase...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20908
更新日期:2006-08-05 00:00:00
abstract::PER.C6 cells were cultivated for propagation of a replication-defective adenovirus vector in serum-free suspension bioreactors. Cellular metabolism during cell growth and adenovirus propagation was fully characterized using on-line and off-line methods. The energy metabolism was found to accelerate transiently after a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20455
更新日期:2005-06-05 00:00:00
abstract::Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10166
更新日期:2002-03-30 00:00:00
abstract::The release kinetics of beta-galactosidase protein have been determined during small-scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramatically affect the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980605)58:5<536::aid-bit
更新日期:1998-06-05 00:00:00
abstract::Reduced downstream costs, together with high purity recovery of polyhydroxyalkanoate (PHA), will accelerate the commercialization of high quality PHA-based products. In this work, a process was designed for effective recovery of the copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) (P(HB-co-HHx)) containing high lev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24713
更新日期:2013-02-01 00:00:00
abstract::Glucose oxidase, invertase, and amyloglucosidase were entrapped in calcium alginate gels as concanavalin A complexes in order to prevent the leaching out of the enzymes from the porous matrix. The free as well as the gel-entrapped concanavalin A-glycoenzyme complexes exhibited a relatively high effectiveness factor, e...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270803
更新日期:1985-08-01 00:00:00
abstract::The heterotrophic marine alga Crypthecodinium cohnii is known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications, during batch cultivation on complex media containing sea salt, yeast extract, and glucose. In the present study, fed-batch cultivation was studied...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10513
更新日期:2003-03-20 00:00:00
abstract::Cartilage implants for potential in vivo use for joint repair or reconstructive surgery can be created in vitro by growing chondrocytes on biodegradable polymer scaffolds. Implants 1 cm in diameter by 0.176 cm thick were made using isolated calf chondrocytes and polyglucolic acid (PGA). By 6 weeks, the total amount of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260430710
更新日期:1994-03-25 00:00:00
abstract::A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23112
更新日期:2011-08-01 00:00:00
abstract::The flotation of Streptomyces pilosus was studied in the absence and presence of adsorbed Pb(II) ions on the cell walls and with and without using sodium dodecylsulfate (SDS) as collector. Adsorption of SDS onto the organism in the absence and presence of Pb(II) ions was also investigated. It was found that in the abs...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260371009
更新日期:1991-04-25 00:00:00
abstract::Biopharmaceutical production would benefit from rapid methods to optimize production of therapeutic proteins by screening host cell line/vector combination, culture media, and operational parameters such as timing of induction. Miniaturized bioreactors are an emerging research area aiming at improving the development ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21751
更新日期:2008-05-01 00:00:00
abstract::An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hy...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22139
更新日期:2009-03-01 00:00:00
abstract::Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320111
更新日期:1988-06-20 00:00:00
abstract::Differences in plasmid retention and expression are studied in both suspended and biofilm cultures of Escherichia coli DH5alpha(PMJR1750). An alternative mathematical model is proposed which allows the determination of plasmid loss probability in both suspended batch and continuously fed biofilm cultures. In our exper...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410207
更新日期:1993-01-20 00:00:00
abstract::The thickness variability of biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thick...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260450607
更新日期:1995-03-20 00:00:00
abstract::The long hydrocarbon fatty acyl chain is energy rich, making it an ideal precursor for liquid transportation fuels and high-value oleo chemicals. As Saccharomyces cerevisiae has many advantages for industrial production compared to Escherichia coli. Here, we attempted to engineer Saccharomyces cerevisiae for overprodu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25239
更新日期:2014-09-01 00:00:00
abstract::Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27536
更新日期:2020-08-18 00:00:00
abstract::A 3D printed ultrafiltration/diafiltration (UF/DF) module is presented allowing the continuous, simultaneous concentration of retained (bio-)molecules and reduction or exchange of the salt buffer. Differing from the single-pass UF concepts known from the literature, DF operation does not require the application of sev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27233
更新日期:2020-03-01 00:00:00
abstract::Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23046
更新日期:2011-06-01 00:00:00
abstract::Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and v...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270112
更新日期:1985-01-01 00:00:00
abstract::Alcaligenes Eutrophus H-16 was grown in continuous culture under conditions which induced hydrogenase production. The hydrogenase enzyme was extracted, partially purified and immobilized on porous glass. This enzyme was then studied both in solution and in immobilized form as a possible candidate for a number of indus...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320306
更新日期:1988-07-20 00:00:00
abstract::A bubble column (0.05 m(3)) and an air-lift fermentor (1.2 m(3)) were used for the production of the exocellular microbial polysaccharide xanthan with Xanthomonas campestris in a synthetic medium. Upon oxygen depletion in the liquid, the xanthan production rate dropped sharply and then became a linear function of the ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390113
更新日期:1992-01-05 00:00:00
abstract::Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260480609
更新日期:1995-12-20 00:00:00
abstract::This paper presents an experimental analysis of matrix bead size distribution and voidage variations with axial height in an expanded bed adsorption system. Use of a specially constructed expanded bed with side ports has enabled sampling from within the expanded bed along the vertical axis. Particles removed from with...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20000920)69:6<648::aid-bit9>3.0.
更新日期:2000-09-20 00:00:00
abstract::Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10548
更新日期:2003-04-05 00:00:00
abstract::Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23289
更新日期:2012-01-01 00:00:00
abstract::Many bacteria used for biotechnological applications are naturally motile. Their "bio-nanopropeller" driven movement allows searching for better environments in a process called chemotaxis. Since bacteria are extremely small in size compared to the bulk fluid volumes in bioreactors, single cell motility is not conside...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21372
更新日期:2007-08-15 00:00:00
abstract::Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22409
更新日期:2009-10-15 00:00:00
abstract::Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22243
更新日期:2009-06-01 00:00:00
abstract::We developed a kinetic model that describes a heterogeneous reaction system for the production of D-p-hydroxyphenylglycine from D,L-p-hydroxyphenyl-hydantoin using D-hydantoinase of Bacillus stearothermophilus SD1 and N-carbamoylase of Agrobacterium tumefaciens NRRL B11291. As a biocatalyst, whole cells with separatel...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10259
更新日期:2002-06-30 00:00:00