Mode of action and properties of the beta-xylosidases from Talaromyces emersonii and Trichoderma reesei.


:Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidases (1,4-beta-D-xylan xylohydrolase, EC from Talaromyces emersonii (betaXTE) and Trichoderma reesei (betaXTR) and a comparison of these in relation to hemicellulose hydrolysis using an industrial relevant substrate. Both beta-xylosidases were expressed in A. oryzae and subsequently purified. During the enzymatic hydrolysis of xylobiose, the reaction product of both enzymes was found to be beta-D-xylose proving that the hydrolysis is proceeding via a retaining reaction mechanism. Based on sequence similarities and glycosyl hydrolases family membership, the active site residues of betaXTE and betaXTR are predicted to be Asp 242 and Glu 441, and Asp 264 and Glu 464, respectively. The involvement in catalysis of these carboxyls was examined by modification using the carbodiimide-nucleophile procedure resulting in a complete inactivation of both enzymes. The degree of xylose release from vinasse, an ethanol fermentation by-product, by betaXTE and betaXTR was 12.1% and 7.7%, respectively. Using the beta-xylosidases in combination with the multicomponent enzyme product Ultraflo L, resulted in 41.9% and 40.8% release of xylose, respectively indicating a strong synergistic effect between the exo-acting beta-xylosidases and the endo-1,4-beta-xylanases and alpha-L-arabinofuranosidase in Ultraflo L. There seems to be no measurable differences between the two beta-xylosidases when used in this specific application despite the differences in specific activity and kinetic properties.


Biotechnol Bioeng


Rasmussen LE,Sørensen HR,Vind J,Viksø-Nielsen A




Has Abstract


2006-08-05 00:00:00












  • The inhibition of Escherichia coli lac operon gene expression by antigene oligonucleotides-mathematical modeling.

    abstract::Gene transcription is regulated by transcription factors that can bind to specific regions on DNA. Antigene oligonucleotides (oligos) can bind to specific regions on DNA and form a triplex with the double-stranded DNA. The triplex can competitively inhibit the binding of transcription factors and, as a result, transcr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cheng B,Fournier RL,Relue PA

    更新日期:2000-11-20 00:00:00

  • Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO2 levels.

    abstract::Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates in...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hakkaart X,Liu Y,Hulst M,El Masoudi A,Peuscher E,Pronk J,van Gulik W,Daran-Lapujade P

    更新日期:2020-03-01 00:00:00

  • Quantitative analysis of biofilm thickness variability.

    abstract::The thickness variability of biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thick...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Murga R,Stewart PS,Daly D

    更新日期:1995-03-20 00:00:00

  • Thiosulphate improves yield of hydrogen production from glucose by the immobilized formate hydrogenlyase system of Escherichia coli.

    abstract::Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nandi R,Dey S,Sengupta S

    更新日期:2001-11-20 00:00:00

  • Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet.

    abstract::Modification of cellular functions by overexpression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ino K,Kawasumi T,Ito A,Honda H

    更新日期:2008-05-01 00:00:00

  • Chemical treatment of Escherichia coli: 1. Extraction of intracellular protein from uninduced cells.

    abstract::Extraction of intracellular protein from Escherichia coli is traditionally achieved by mechanical disruption. A chemical treatment that destroys the integrity of the bacterial cell wall and could provide an alternative technique is examined in this study. Treatment with a combination of the chelating agent ethylenedia...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Falconer RJ,O'Neill BK,Middelberg AP

    更新日期:1997-03-05 00:00:00

  • Redesigning metabolic networks using mathematical programming

    abstract::The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dean JP,Dervakos GA

    更新日期:1998-04-05 00:00:00

  • Biomass evolution in porous media and its effects on permeability under starvation conditions.

    abstract::The purpose of this study was to understand bacteria profile modification and its applications in subsurface biological operations such as biobarrier formation, in situ bioremediation, and microbial-enhanced oil recovery. Biomass accumulation and evolution in porous media were investigated both experimentally and theo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim DS,Fogler HS

    更新日期:2000-07-05 00:00:00

  • Predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems.

    abstract::A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes while the electrostat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sebastião MJ,Martel P,Baptista A,Petersen SB,Cabral JM,Aires-Barros MR

    更新日期:1997-11-05 00:00:00

  • Proposed mechanism of acetate accumulation in two recombinant Escherichia coli strains during high density fermentation.

    abstract::The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (lambdaDE3) and JM109, differ significantly in their acetate production during batch fermentation at high initial glucose con...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: van de Walle M,Shiloach J

    更新日期:1998-01-05 00:00:00

  • Simulations of host-plasmid interactions in Escherichia coli: Copy number, promoter strength, and ribosome binding site strength effects on metabolic activity and plasmid gene expression.

    abstract::A mechanistically detailed single-cell model E. coli B/r-A was adapted to simulate the effects of vector presence on cell metabolism. Competition for RNA polymerase between chromosome and plasmid DNA is explicitly included. Distribution of active ribosomes among chromosome- and plasmid-derived messenger RNA, another k...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Peretti SW,Bailey JE

    更新日期:1987-02-01 00:00:00

  • Performance modeling and simulation of biochemical process sequences with interacting unit operations.

    abstract::Many biochemical processes consist of a sequence of operations for which optimal operating conditions (setpoints) have to be determined. If such optimization is performed for each operation separately with respect to objectives defined for each operation individually, overall process performance is likely to be subopt...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Groep ME,Gregory ME,Kershenbaum LS,Bogle ID

    更新日期:2000-02-05 00:00:00

  • Correlating single cell motility with population growth dynamics for flagellated bacteria.

    abstract::Many bacteria used for biotechnological applications are naturally motile. Their "bio-nanopropeller" driven movement allows searching for better environments in a process called chemotaxis. Since bacteria are extremely small in size compared to the bulk fluid volumes in bioreactors, single cell motility is not conside...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Arora S,Bhat V,Mittal A

    更新日期:2007-08-15 00:00:00

  • Increased metal tolerance and bioaccumulation of zinc and cadmium in Chlamydomonas reinhardtii expressing a AtHMA4 C-terminal domain protein.

    abstract::The use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM C...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ibuot A,Webster RE,Williams LE,Pittman JK

    更新日期:2020-10-01 00:00:00

  • Kinetics of whey-lactose acidogenesis.

    abstract::The mixed-culture anaerobic conversion of lactose to organic acids in a bench-scale continuous-flow stirredtank fermentor is considered. The major acidogenic end-product distribution with respect to the dilution rate are presented. A Monod chemostat model is employed to describe a microbial growth, and the influence o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kissalita WS,Lo KV,Pinder KL

    更新日期:1989-01-25 00:00:00

  • Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin.

    abstract::Cancer is the leading cause of death in industrialized countries. Cancer therapy often involves monoclonal antibodies or small-molecule drugs, but carbohydrate-binding lectins such as mistletoe (Viscum album) viscumin offer a potential alternative treatment strategy. Viscumin is toxic in mammalian cells, ruling them o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gengenbach BB,Keil LL,Opdensteinen P,Müschen CR,Melmer G,Lentzen H,Bührmann J,Buyel JF

    更新日期:2019-09-01 00:00:00

  • Saccharification of bamboo carbohydrates for the production of ethanol.

    abstract::Bamboo carbohydrates were hydrolyzed with commercial amylases and a mixture of fungal culture broths containing cellulolytic and hemicellulolytic enzymes. The effects of cooking temperature and the size of fiber particles were also investigated. It was found that the higher the cooking temperature, the higher the rate...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: De Menezes TJ,Dos Santos CL,Azzini A

    更新日期:1983-04-01 00:00:00

  • Accurate O2 delivery enabled benzene biodegradation through aerobic activation followed by denitrification-coupled mineralization.

    abstract::Although benzene can be biodegraded when dissolved oxygen is sufficient, delivering oxygen is energy intensive and can lead to air stripping the benzene. Anaerobes can biodegrade benzene by using electron acceptors other than O2 , and this may reduce costs and exposure risks; the drawback is a remarkably slower growth...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Liu Z,Zhou C,Ontiveros-Valencia A,Luo YH,Long M,Xu H,Rittmann BE

    更新日期:2018-08-01 00:00:00

  • Prediction of the profiles of the elution bands of proteins in preparative liquid chromatography.

    abstract::A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isoc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Katti A,Huang JX,Guiochon G

    更新日期:1990-07-01 00:00:00

  • Maximum production strategy for biodegradable copolymer P(HB-co-HV) in fed-batch culture of Alcaligenes eutrophus.

    abstract::A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shimizu H,Kozaki Y,Kodama H,Shioya S

    更新日期:1999-03-05 00:00:00

  • Non-invasive mass transfer measurements in complex biofilm-coated structures.

    abstract::We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Graf von der Schulenburg DA,Akpa BS,Gladden LF,Johns ML

    更新日期:2008-10-15 00:00:00

  • Immobilization of proteins on organic polymer beads.

    abstract::A new method is described for the immobilization of biologically active proteins onto several types of organic polymer beads. First, the soluble protein is modified by reaction with an excess of a hydrophobic imidoester, for example methyl 4-phenyl-butyrimidate, at ca. pH 9 and 0 degrees . Excess imidoester and side p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ampon K,Means GE

    更新日期:1988-08-20 00:00:00

  • Magnetic resonance microscopy analysis of advective transport in a biofilm reactor.

    abstract::In this article we present magnetic resonance microscopy (MRM) characterization of the advective transport in a biofilm capillary reactor. The biofilm generates non-axial flows that are up to 20% of the maximum axial velocity. The presence of secondary velocities of this magnitude alters the mass transport in the bior...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gjersing EL,Codd SL,Seymour JD,Stewart PS

    更新日期:2005-03-30 00:00:00

  • Kinetics of organic compound removal from waste gases with a biological filter.

    abstract::In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ottengraf SP,Van Den Oever AH

    更新日期:1983-12-01 00:00:00

  • Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors.

    abstract::The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

    更新日期:2006-08-20 00:00:00

  • Production of cell culture (MDCK) derived live attenuated influenza vaccine (LAIV) in a fully disposable platform process.

    abstract::The majority of influenza vaccines are manufactured using embryonated hens' eggs. The potential occurrence of a pandemic outbreak of avian influenza might reduce or even eliminate the supply of eggs, leaving the human population at risk. Also, the egg-based production technology is intrinsically cumbersome and not eas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: George M,Farooq M,Dang T,Cortes B,Liu J,Maranga L

    更新日期:2010-08-15 00:00:00

  • The production of cellulase in a spouted bed fermentor using cells immobilized in biomass support particles.

    abstract::Continuous cellulase production by Trichoderma viride QM 9123, immobilized in 6 mm diameter, spherical, stainless steel biomass support particles, has been achieved using a medium containing glucose as the main carbon source. Experiments were carried out in a 10-L spouted bed fermentor. In this type of reactor-recycle...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Webb C,Fukuda H,Atkinson B

    更新日期:1986-01-01 00:00:00

  • Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris.

    abstract::Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol-induced AOX1 promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes glycerol and biomass is accumulated; in th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trinh LB,Phue JN,Shiloach J

    更新日期:2003-05-20 00:00:00

  • Action of shear on enzymes: studies with alcohol dehydrogenase.

    abstract::Yeast alcohol dehydrogenase (ADH) solutions (approximately 1 mg/ml, pH 7) were sheared in a coaxial cylindrical viscometer. This was fitted with a lid sealing the contents from the atmosphere and preventing evaporation. At 30 degrees C after a total of 5 hr intermittent shearing at 683 sec-1 no losses of activity were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Thomas CR,Nienow AW,Dunnill P

    更新日期:1979-12-01 00:00:00

  • YATP value in Candida tropicalis grown on n-alkanes, fatty acids, and acetate.

    abstract::The amount of ATP produced during n-alkane, fatty acid, or acetate metabolism in Candida tropicalis has been established from the P/O ratios measured on isolated mitochondria, yield on substrate and carbon balance. For these three kinds of substrates YATP value has been found to be close to 4, although Ysub on acetate...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gallo M,Azoulay E

    更新日期:1975-12-01 00:00:00