Mode of action and properties of the beta-xylosidases from Talaromyces emersonii and Trichoderma reesei.


:Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidases (1,4-beta-D-xylan xylohydrolase, EC from Talaromyces emersonii (betaXTE) and Trichoderma reesei (betaXTR) and a comparison of these in relation to hemicellulose hydrolysis using an industrial relevant substrate. Both beta-xylosidases were expressed in A. oryzae and subsequently purified. During the enzymatic hydrolysis of xylobiose, the reaction product of both enzymes was found to be beta-D-xylose proving that the hydrolysis is proceeding via a retaining reaction mechanism. Based on sequence similarities and glycosyl hydrolases family membership, the active site residues of betaXTE and betaXTR are predicted to be Asp 242 and Glu 441, and Asp 264 and Glu 464, respectively. The involvement in catalysis of these carboxyls was examined by modification using the carbodiimide-nucleophile procedure resulting in a complete inactivation of both enzymes. The degree of xylose release from vinasse, an ethanol fermentation by-product, by betaXTE and betaXTR was 12.1% and 7.7%, respectively. Using the beta-xylosidases in combination with the multicomponent enzyme product Ultraflo L, resulted in 41.9% and 40.8% release of xylose, respectively indicating a strong synergistic effect between the exo-acting beta-xylosidases and the endo-1,4-beta-xylanases and alpha-L-arabinofuranosidase in Ultraflo L. There seems to be no measurable differences between the two beta-xylosidases when used in this specific application despite the differences in specific activity and kinetic properties.


Biotechnol Bioeng


Rasmussen LE,Sørensen HR,Vind J,Viksø-Nielsen A




Has Abstract


2006-08-05 00:00:00












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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vergara-Fernández A,Hernández S,Revah S

    更新日期:2011-04-01 00:00:00

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cayli A,Hirschmann F,Wirth M,Hauser H,Wagner R

    更新日期:1999-10-20 00:00:00

  • A kinetic model of quantitative real-time polymerase chain reaction.

    abstract::Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mehra S,Hu WS

    更新日期:2005-09-30 00:00:00

  • A novel recycle batch immobilized cell bioreactor for propionate production from whey lactose.

    abstract::Recycle batch fermentations using immobilized cells of Propionibacterium acidipropionici were studied for propionate production from whey permeate, de-lactose whey permeate, and acid whey. Cells were immobilized in a spirally wound fibrous sheet packed in a 0.5-L column reactor, which was connected to a 5-L stirred ta...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yang ST,Huang Y,Hong G

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mauricio JC,Ortega JM

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xia F,Nagrath D,Garde S,Cramer SM

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Boireau W,Bombard S,Sari MA,Pompon D

    更新日期:2002-01-20 00:00:00

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kempe H,Persson P,Axelsson A,Nilsson B,Zacchi G

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schmitz C,Pepelanova I,Seliktar D,Potekhina E,Belousov VV,Scheper T,Lavrentieva A

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rozzi A,Castellazzi L,Speece RE

    更新日期:2002-01-05 00:00:00

  • Encapsulation of plant growth-promoting bacteria in alginate beads enriched with humic acid.

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    journal_title:Biotechnology and bioengineering

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    authors: Young CC,Rekha PD,Lai WA,Arun AB

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    journal_title:Biotechnology and bioengineering

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    authors: Tanadchangsaeng N,Yu J

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    authors: Tyagi RD,Ghose TK

    更新日期:1982-04-01 00:00:00

  • Impact of external calcium and calcium sensors on ginsenoside Rb1 biosynthesis by Panax notoginseng cells.

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    journal_title:Biotechnology and bioengineering

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    authors: Yue CJ,Zhong JJ

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    pub_type: 杂志文章


    authors: Alberghina L,Mariani L,Martegani E,Vanoni M

    更新日期:1983-05-01 00:00:00

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    journal_title:Biotechnology and bioengineering

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    authors: Martin CK,Perlman D

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    journal_title:Biotechnology and bioengineering

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    authors: Sabalowsky AR,Semprini L

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  • Simple diffusion is the primary mechanism for glucose uptake during the production phase of the Aspergillus niger citric acid process.

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    authors: Wayman FM,Mattey M

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    authors: Jenkins N,Hovey A

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  • An immobilized hydrogenase from Alcaligenes eutrophus H-16.

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    authors: Baac H,Hajós JP,Lee J,Kim D,Kim SJ,Shuler ML

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    authors: Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

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    authors: Schuler AJ

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Menzel K,Ahrens K,Zeng A,Deckwer W

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