Feasibility of enzymatic hydrolysis and alcoholic fermentation of starch contained in buffalo gourd (Cucurbita foetidissima) roots.

abstract：:Expression of recombinant proteins in Escherichia coli often results in the formation of insoluble inclusion bodies, In case of expression of eukaryotic proteins containing cysteine, which may form disulfide bonds in the native active protein, often nonnative inter- and intramolecular disulfide bonds exist in the incl...

journal_title：Biotechnology and bioengineering

authors： Fischer B,Sumner I,Goodenough P

更新日期：1993-01-05 00:00:00

abstract：:This paper presents a new formulation for the analysis of growth kinetics on multiple nutrients. The baseline for the theory is the concept that a noninteractive growth process occurs among perfectly substitutable nutrients if the locus of points of the substrate concentrations producing equal growth rate is linear. A...

journal_title：Biotechnology and bioengineering

authors： Haas CN

更新日期：1994-06-20 00:00:00

abstract：:In large-scale mammalian cell culture, the key toxic by-products assessed and monitored are lactate and ammonia. Often no distinction between the two isoforms of lactate is made. Here, we present profiles of both D- and L-lactate. D-Lactate is the end molecule of the methylglyoxal pathway. D-Lactate unlike L-lactate i...

journal_title：Biotechnology and bioengineering

authors： Paoli T,Faulkner J,O'kennedy R,Keshavarz-Moore E

更新日期：2010-09-01 00:00:00

abstract：:Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

journal_title：Biotechnology and bioengineering

authors： Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

更新日期：2019-01-01 00:00:00

abstract：:The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass without allowing glucose to be diverted into the reductive meta...

journal_title：Biotechnology and bioengineering

authors： Duboc P,Cascão-Pereira LG,von Stockar U

更新日期：1998-03-05 00:00:00

abstract：:A method for purifying alpha-amylase inhibitor from wheat meal based on immobilized metal affinity with a thermosensitive copolymer is developed. The studies represent the thermoprecipitation properties of the copolymers of N-isopropylacrylamide (NIPAM) with iminodiacetic acid (IDA) and 1-vinylimidazole (VI), respecti...

journal_title：Biotechnology and bioengineering

authors： Kumar A,Galaev IY,Mattiasson B

更新日期：1998-09-20 00:00:00

abstract：:Effective enzyme-mediated viscosity reduction, disaggregation, or "liquefaction," is required to overcome the rheological challenges resulting from the fibrous, hygroscopic nature of lignocellulosic biomass, particularly at the high solids loadings that will be required for an economically viable process. However, the...

journal_title：Biotechnology and bioengineering

authors： van der Zwan T,Hu J,Saddler JN

更新日期：2017-11-01 00:00:00

abstract：:Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...

journal_title：Biotechnology and bioengineering

authors： Yang Y,Tsifansky MD,Shin S,Lin Q,Yeo Y

更新日期：2011-06-01 00:00:00

abstract：:A novel chemistry has been developed for the production of capsules composed of a hydrophobic liquid core surrounded by a cross-linked polyacrylamide/alginate membrane. These liquid-core capsules may be used in capsular perstraction for the removal of inhibitory products from bioprocesses and bioconversions. They have...

journal_title：Biotechnology and bioengineering

authors： Wyss A,von Stockar U,Marison IW

更新日期：2004-06-05 00:00:00

abstract：:A spinning cylindrical filter, known as a spinfilter, permits the mammalian cell bioreactor operation at high perfusion rates leading to very high cell densities (10(7) mL(-1)). Filter screens with openings (25 mum) slightly larger than the average cell size have been used to retain single cells in suspension over a l...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1994-01-20 00:00:00

abstract：:Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and p...

journal_title：Biotechnology and bioengineering

authors： Dipasquantonio VM,Betenbaugh MJ,Dhurjati P

更新日期：1987-03-01 00:00:00

abstract：:The enzymatic synthesis of polyesters from activated diesters and diols has been investigated. Differences between enzymatic synthesis and traditional chemical condensation processes are discussed. The disappearance of monomers during the initial phase of reaction indicates that enzyme has a higher specificity for tra...

journal_title：Biotechnology and bioengineering

authors： Chaudhary AK,Beckman EJ,Russell AJ

更新日期：1997-07-05 00:00:00

abstract：:The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulose powder concentration in the nonwoven materials was more than 4%, cellulase p...

journal_title：Biotechnology and bioengineering

authors： Tamada M,Kasai N,Kumakura M,Kaetsu I

更新日期：1986-08-01 00:00:00

abstract：:Cells usually spread on a synthetic substrate through bonds between receptors and chemical groups on the substrate (ligands). Therefore, it is valuable to study the effects of the average number density of these chemical groups and the average distance between them to model and predict the cell behavior. Poly(ethylene...

journal_title：Biotechnology and bioengineering

authors： Eskandari M,Brey E,Cinar A

更新日期：2011-02-01 00:00:00

abstract：:Helical-blade solids mixers have a large potential as bioreactors for solid-state fermentation (SSF). Fundamental knowledge of the flow and mixing behavior is required for robust operation of these mixers. In this study predictions of a discrete particle model were compared to experiments with colored wheat grain part...

journal_title：Biotechnology and bioengineering

authors： Schutyser MA,Briels WJ,Rinzema A,Boom RM

更新日期：2003-10-05 00:00:00

abstract：:An extensive investigation of anaerobic methane fermentation requires identifying the relationship between the physical environment and biological process. In this study, a computational fluid dynamics (CFD) technique was used to characterize bacterial fermentation mechanisms intertwined with mixing and heat transfer ...

journal_title：Biotechnology and bioengineering

authors： Wu B

更新日期：2012-11-01 00:00:00

abstract：:A semimechanistic multi-reaction kinetic model was developed to describe the enzymatic hydrolysis of a lignocellulosic biomass, creeping wild ryegrass (CWR; Leymus triticoides). This model incorporated one homogeneous reaction of cellobiose-to-glucose and two heterogeneous reactions of cellulose-to-cellobiose and cell...

journal_title：Biotechnology and bioengineering

authors： Zheng Y,Pan Z,Zhang R,Jenkins BM

更新日期：2009-04-15 00:00:00

abstract：:Static and dynamic characteristics of continuous cultures of recombinant methylotrophs, which are designed to improve the selectivity of plasmid-bearing cells and the plasmid stability, are investigated in detail. Operational regions in which coexistence (survival of plasmid-bearing and plasmid-free cells) operation i...

journal_title：Biotechnology and bioengineering

authors： Parulekar SJ,Chang YK,Modak JM,Lim HC

更新日期：1987-06-01 00:00:00

abstract：:Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...

journal_title：Biotechnology and bioengineering

authors： Ren H,Hu P,Zhao H

更新日期：2017-08-01 00:00:00

abstract：:The application of rational design in reallocating metabolic flux to accumulate desired chemicals is always restricted by the native regulatory network. In this study, recombinant Pichia pastoris was constructed for malic acid production from sole methanol through rational redistribution of metabolic flux. Different m...

journal_title：Biotechnology and bioengineering

authors： Guo F,Dai Z,Peng W,Zhang S,Zhou J,Ma J,Dong W,Xin F,Zhang W,Jiang M

更新日期：2021-01-01 00:00:00

abstract：:In this article, we consider the impact on downstream process design resulting from the use of metabolically engineered yeast strains. We address the issue of how manipulation of cell wall permeability can improve the release and subsequent recovery of heterologous products produced in yeast. ...

journal_title：Biotechnology and bioengineering

authors： Ferrer P,Diers I,Asenjo JA,Andrews BA

更新日期：1998-04-20 00:00:00

abstract：:Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...

journal_title：Biotechnology and bioengineering

authors： Choi MH,Xu J,Rho JK,Shim JH,Yoon SC

更新日期：2009-03-01 00:00:00

abstract：:An integrated approach is used to develop a rapid sampling strategy for the quantitative analysis of in vivo kinetic behavior based on measured concentrations of intracellular metabolites in Saccharomyces cerevisiae. Emphasis is laid on small sample sizes during sampling and analysis. Subsecond residence times are acc...

journal_title：Biotechnology and bioengineering

authors： Lange HC,Eman M,van Zuijlen G,Visser D,van Dam JC,Frank J,de Mattos MJ,Heijnen JJ

更新日期：2001-11-20 00:00:00

abstract：:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined i...

journal_title：Biotechnology and bioengineering

authors： Kimura M,Michizoe J,Oakazaki SY,Furusaki S,Goto M,Tanaka H,Wariishi H

更新日期：2004-11-20 00:00:00

abstract：:Neural networks are formed by accurate connectivity of neurons and glial cells in the brain. These networks employ a three-dimensional bio-surface that both assigns precise coordinates to cells during development and facilitates their connectivity and functionality throughout life. Using specific topographic and chemi...

journal_title：Biotechnology and bioengineering

authors： Bani-Yaghoub M,Tremblay R,Voicu R,Mealing G,Monette R,Py C,Faid K,Sikorska M

更新日期：2005-11-05 00:00:00

abstract：:We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

journal_title：Biotechnology and bioengineering

authors： Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

更新日期：2017-08-01 00:00:00

abstract：:Although several compelling benefits for bioprocess intensification have been reported, the need for a streamlined integration of perfusion cultures with capture chromatography still remains unmet. Here, a robust solution is established by conducting tangential flow filtration-based perfusion with a wide-surface pore ...

journal_title：Biotechnology and bioengineering

authors： Pinto NDS,Brower M

更新日期：2020-07-15 00:00:00

abstract：:The anaerobic oxidation of ammonium (anammox) uses nitrite as terminal electron acceptor. The nitrite can cause inhibition to the bacteria that catalyze the anammox reaction. The literature shows a great divergence on the levels of NO2 (-) causing inhibition. Moreover, the conditions influencing the resistance of anam...

journal_title：Biotechnology and bioengineering

authors： Carvajal-Arroyo JM,Puyol D,Li G,Sierra-Álvarez R,Field JA

更新日期：2014-10-01 00:00:00

abstract：:Bioluminescence from the lux-based bacterial reporter Pseudomonas fluorescens HK44 was experimentally investigated under growth substrate-rich and limiting conditions in batch, continuous stirred tank (CSTR), and turbidostat reactors. A mechanistically based, mathematical model was developed to describe bioluminescenc...

journal_title：Biotechnology and bioengineering

authors： Kelly CJ,Hsiung CJ,Lajoie CA

更新日期：2003-02-05 00:00:00

abstract：:A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...

journal_title：Biotechnology and bioengineering

authors： Senerovic L,Stankovic N,Spizzo P,Basso A,Gardossi L,Vasiljevic B,Ljubijankic G,Tisminetzky S,Degrassi G

更新日期：2006-02-05 00:00:00