Shifting of the distribution of aromatic monomer-units in polyhydroxyalkanoic acid to longer units by salicylic acid in Pseudomonas fluorescens BM07 grown with mixtures of fructose and 11-phenoxyundecanoic acid.


:Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACPs derived from acetyl-CoA to coenzyme A. It was found that salicylic acid (SA), in a concentration dependent manner, suppressed the accumulation of PHA in Pseudomonas fluorescens BM07 from fructose as well as shifted the distribution of monomer-units derived from a MCL fatty acid co-added as carbon source (e.g., 11-phenoxyundecanoic acid (11-POU)) to longer monomer-units. Both SA and acrylic acid were found to induce high accumulations of 3-ketohexanoic acid in BM07 wild-type cells grown with n-hexanoic acid as well as to inhibit the formation of acetyl-CoA from acetoacetyl-CoA by BM07 cell extract, suggesting that 3-ketoacyl-CoA thiolase is their common beta-oxidation target. The structural motif of acrylic acid present in the molecular structure of SA may self-explain the similar actions of the two inhibitors. A comparison of monomer modulation between BM07 wild-type and DeltaphaG mutant cells grown on the mixtures of fructose and 11-POU revealed that both PhaG and beta-oxidation inhibitor may play a critical role in the synthesis of PHA with longer side-chain omega-functional substitutions.


Biotechnol Bioeng


Choi MH,Xu J,Rho JK,Shim JH,Yoon SC




Has Abstract


2009-03-01 00:00:00












  • Extracellular hemoglobin combined with an O2 -generating material overcomes O2 limitation in the bioartificial pancreas.

    abstract::The bioartificial pancreas encapsulating pancreatic islets in immunoprotective hydrogel is a promising therapy for Type 1 diabetes. As pancreatic islets are highly metabolically active and exquisitely sensitive to hypoxia, maintaining O2 supply after transplantation remains a major challenge. In this study, we address...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mouré A,Bacou E,Bosch S,Jegou D,Salama A,Riochet D,Gauthier O,Blancho G,Soulillou JP,Poncelet D,Olmos E,Bach JM,Mosser M

    更新日期:2019-05-01 00:00:00

  • Biofilm thickness variability investigated with a laser triangulation sensor.

    abstract::Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Okkerse WJ,Ottengraf SP,Osinga-Kuipers B

    更新日期:2000-12-20 00:00:00

  • Bioartificial kidney. I. Theoretical analysis of convective flow in hollow fiber modules: application to a bioartificial hemofilter.

    abstract::Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Moussy Y

    更新日期:2000-04-20 00:00:00

  • A CRISPR-Cas12a-Derived Biosensor Enabling Portable Personal Glucose Meter Readout for Quantitative Detection of SARS-CoV-2.

    abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly throughout the whole world and caused significant difficulties in the prevention and control of the epidemic. In this case, several detection methods have been established based on nucleic acid diagnostic techniques and immunoassays to ach...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Huang D,Shi Z,Qian J,Bi K,Fang M,Xu Z

    更新日期:2021-01-06 00:00:00

  • Kinetic and thermal characteristics of enzyme-graft copolymers.

    abstract::The characteristics of horseradish peroxidase (HRP) immobilized onto Sepharose by a photochemical-initiated graft copolymerization are presented. Active copolymers were synthesized using different amounts of glycidylmethacrylate (GMA), bisacryloylpiperazine (BAP), or 1,3,5-hexhydrotriacryloyl-(s)-triazine (HTsT) as fu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cremonesi P,D'Angiuro L

    更新日期:1983-03-01 00:00:00

  • Fed-batch fermentor synthesis of 3-dehydroshikimic acid using recombinant Escherichia coli.

    abstract::3-Dehydroshikimic acid (DHS), in addition to being a potent antioxidant, is the key hydroaromatic intermediate in the biocatalytic conversion of glucose into aromatic bioproducts and a variety of industrial chemicals. Microbial synthesis of DHS, like other intermediates in the common pathway of aromatic amino acid bio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Li K,Mikola MR,Draths KM,Worden RM,Frost JW

    更新日期:1999-07-05 00:00:00

  • Analysis of metabolic pathways and fluxes in a newly discovered thermophilic and ethanol-tolerant Geobacillus strain.

    abstract::A recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C-ba...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tang YJ,Sapra R,Joyner D,Hazen TC,Myers S,Reichmuth D,Blanch H,Keasling JD

    更新日期:2009-04-01 00:00:00

  • A nanosensory device fabricated on a liposome for detection of chemical signals.

    abstract::This paper describes construction of a nanosensory device for amplified detection of biologically important amines as chemical signals. The device was inspired by a biological signal transduction system, and was fabricated on an artificial cell membrane through self-organization of the molecular components, such as a ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sasaki Y,Shioyama Y,Tian WJ,Kikuchi J,Hiyama S,Moritani Y,Suda T

    更新日期:2010-01-01 00:00:00

  • Quantification of proteomic and metabolic burdens predicts growth retardation and overflow metabolism in recombinant Escherichia coli.

    abstract::Escherichia coli has been the host organism most frequently investigated for efficient recombinant protein production. However, the production of a foreign protein in recombinant E. coli often leads to growth deterioration and elevated secretion of acetic acid. Such observed phenomena have been widely linked with cell...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zeng H,Yang A

    更新日期:2019-06-01 00:00:00

  • Design of reactive porous polymer supports for high throughput bioreactors: poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide- co-ethylene dimethacrylate) monoliths.

    abstract::Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlacto...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xie S,Svec F,Fréchet JM

    更新日期:1999-01-05 00:00:00

  • Fluorophore-labeled carbohydrate analysis of immunoglobulin fusion proteins: Correlation of oligosaccharide content with in vivo clearance profile.

    abstract::CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Flesher AR,Marzowski J,Wang WC,Raff HV

    更新日期:1995-06-05 00:00:00

  • Theoretical distribution of truncation lengths in incremental truncation libraries.

    abstract::Incremental truncation is a method for constructing libraries of every one base pair truncation of a segment of DNA. Incremental truncation libraries can be created using a time-dependent nuclease method or through the incorporation of alpha-phosphothioate dNTPs by PCR or by primer extension (THIO(pcr) truncation and ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ostermeier M

    更新日期:2003-06-05 00:00:00

  • Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris.

    abstract::Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol-induced AOX1 promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes glycerol and biomass is accumulated; in th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trinh LB,Phue JN,Shiloach J

    更新日期:2003-05-20 00:00:00

  • The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study.

    abstract::At the laboratory scale, sudden step increases from 30 to 42 degrees C can be readily accomplished when expressing heterologous proteins in heat-inducible systems. However, for large scale-cultures only slow ramp-type increases in temperature are possible due to heat transfer limitations, where the heating rate decrea...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Caspeta L,Flores N,Pérez NO,Bolívar F,Ramírez OT

    更新日期:2009-02-01 00:00:00

  • A study of D-lactate and extracellular methylglyoxal production in lactate re-utilizing CHO cultures.

    abstract::In large-scale mammalian cell culture, the key toxic by-products assessed and monitored are lactate and ammonia. Often no distinction between the two isoforms of lactate is made. Here, we present profiles of both D- and L-lactate. D-Lactate is the end molecule of the methylglyoxal pathway. D-Lactate unlike L-lactate i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Paoli T,Faulkner J,O'kennedy R,Keshavarz-Moore E

    更新日期:2010-09-01 00:00:00

  • Effects of pH and aeration on gamma-poly(glutamic acid) formation by Bacillus licheniformis in controlled batch fermentor cultures.

    abstract::Bacillus licheniformis ATCC 9945A was grown on Medium E in batch fermentations in which the pH was maintained at 5.5., 6.5, 7.4, and 8.25. The effects of pH on cell growth, carbon source utilization, and gamma-polyglutamic acid (gamma-PGA) production, molecular weight, and polymer stereochemistry were determined. The ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cromwick AM,Birrer GA,Gross RA

    更新日期:1996-04-20 00:00:00

  • Functional phenotyping of human plasma using a 361-fluorogenic substrate biosensing microarray.

    abstract::A microarray presenting glycerol nanodroplets of fluorogenic peptide substrates was used as a biosensor for the detection of multiple enzyme activities within human plasma. Using 10 different plasma proteases (kallikrein, factor XIIa, factor XIa, factor IXa, factor VIIa, factor Xa, thrombin, activated protein C, uPA a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gosalia DN,Denney WS,Salisbury CM,Ellman JA,Diamond SL

    更新日期:2006-08-20 00:00:00

  • The effects of methanol on the biofiltration of dimethyl sulfide in inorganic biofilters.

    abstract::Air emissions from the pulp and paper industry frequently contain reduced sulfur compounds (RSC), such as dimethyl sulfide (DMS) mixed with volatile organic compounds (VOC) (e.g., methanol, MeOH) and it is desirable to treat either one or both of these groups of compounds. The objective of this study was to assess the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang Y,Liss SN,Allen DG

    更新日期:2006-11-05 00:00:00

  • Characterization of hybridoma cell responses to elevated pCO(2) and osmolality: intracellular pH, cell size, apoptosis, and metabolism.

    abstract::CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: deZengotita VM,Schmelzer AE,Miller WM

    更新日期:2002-02-15 00:00:00

  • Comment on mathematical models which describe transcription and calculate the relationship between mRNA and protein expression ratio.

    abstract::Mathematical models to describe transcription (Arnold et al. (2001); Biotech Bioeng 72:548-561) and translation (Mehra et al. (2003); Biotech Bioeng 84:822-841) in bacteria are modified in order to improve reaction kinetics and to include the number of polymerase molecules that are active on the DNA, as well as to inc...

    journal_title:Biotechnology and bioengineering

    pub_type: 评论,信件


    authors: Kremling A

    更新日期:2007-03-01 00:00:00

  • Gas sensing in microplates with optodes: influence of oxygen exchange between sample, air, and plate material.

    abstract::Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Arain S,Weiss S,Heinzle E,John GT,Krause C,Klimant I

    更新日期:2005-05-05 00:00:00

  • A simple model describing nitrate and nitrite reduction in fluidized bed biological reactors.

    abstract::A uniquely simple model is developed to describe the NO(3) (-) and NO(2) (-) concentration profiles within a dentrification fluidized bed biological reactor. This simple model is compared to experimental data, and to a more complex model similar to those previously proposed in the literature. The simple model fits the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brosilow BJ,Schnitzer M,Tarre S,Green M

    更新日期:1997-06-20 00:00:00

  • Operational stability of copolymerized enzymes at elevated temperatures.

    abstract::The copolymerization method of immobilization was used to obtain preparations of enzymes covalently incorporated in polyacrylamide gel. They possess properties making them suitable for practical use. First, the preparations are hundreds of times more stable against irreversible thermoinactivation than native enzymes. ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mozhaev VV,Siksnis VA,Torchilin VP,Martinek K

    更新日期:1983-08-01 00:00:00

  • Application of hydrophobic interaction displacement chromatography for an industrial protein purification.

    abstract::Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sunasara KM,Xia F,Gronke RS,Cramer SM

    更新日期:2003-05-05 00:00:00

  • Fluorescent silica particles for monitoring oxygen levels in three-dimensional heterogeneous cellular structures.

    abstract::Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations w...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Acosta MA,Velasquez M,Williams K,Ross JM,Leach JB

    更新日期:2012-10-01 00:00:00

  • Simple approach to reducing proteolysis during secretory production of human parathyroid hormone in Saccharomyces cerevisiae.

    abstract::A gene coding for human parathyroid hormone (hPTH) was synthesized and cloned into a yeast expression and secretion vector containing the mating factor alpha pre-pro leader sequence and the galactose-inducible promoter, GAL10. The intact hPTH(1-84) was found to be secreted into the culture medium. As observed in the p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chung BH,Park KS

    更新日期:1998-01-20 00:00:00

  • Establishing the scalable manufacture of primary human T-cells in an automated stirred-tank bioreactor.

    abstract::Advanced cell and gene therapies such as chimeric antigen receptor T-cell immunotherapies (CAR-T), present a novel therapeutic modality for the treatment of acute and chronic conditions including acute lymphoblastic leukemia and non-Hodgkin lymphoma. However, the development of such immunotherapies requires the manufa...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Costariol E,Rotondi M,Amini A,Hewitt CJ,Nienow AW,Heathman TRJ,Micheletti M,Rafiq QA

    更新日期:2019-10-01 00:00:00

  • Surfactant-Induced hydrogen production in cyanobacteria.

    abstract::Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H(2)/h . mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Famiglietti M,Hochkoeppler A,Luisi PL

    更新日期:1993-10-01 00:00:00

  • Virus detection using filament-coupled antibodies.

    abstract::Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Stone GP,Mernaugh R,Haselton FR

    更新日期:2005-09-20 00:00:00

  • Immobilization of thermolysin to polyamide nonwoven materials.

    abstract::In the last few years, an increasing number of biotechnological techniques have been applied to the restoration and conservation of works of art, paintings, old maps, and papers or books. Enzymes can solve problems that give restorers difficulties, although for many applications it is not possible to use soluble enzym...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Moeschel K,Nouaimi M,Steinbrenner C,Bisswanger H

    更新日期:2003-04-20 00:00:00