Metabolic engineering of Pichia pastoris for malic acid production from methanol.

abstract：:Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...

journal_title：Biotechnology and bioengineering

authors： Skardal A,Devarasetty M,Forsythe S,Atala A,Soker S

更新日期：2016-09-01 00:00:00

abstract：:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especia...

journal_title：Biotechnology and bioengineering

authors： Guiraud JP,Bourgi J,Stervinou M,Claisse M,Galzy P

更新日期：1987-05-01 00:00:00

abstract：:Incremental truncation is a method for constructing libraries of every one base pair truncation of a segment of DNA. Incremental truncation libraries can be created using a time-dependent nuclease method or through the incorporation of alpha-phosphothioate dNTPs by PCR or by primer extension (THIO(pcr) truncation and ...

journal_title：Biotechnology and bioengineering

authors： Ostermeier M

更新日期：2003-06-05 00:00:00

abstract：:Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...

journal_title：Biotechnology and bioengineering

authors： Li XM,Liang BS,Wang HY

更新日期：1988-02-20 00:00:00

abstract：:Hairy root cultures of Catharanthus roseus were established by infection of seedlings with Agrobacterium rhizogenes 15834. Approximately 150 transformants from four different. C. roseus cultivars were screened for desirable traits in growth and indole alkaloid production. Five hairy root clones grew well in liquid cul...

journal_title：Biotechnology and bioengineering

authors： Bhadra R,Vani S,Shanks JV

更新日期：1993-03-05 00:00:00

abstract：:A general framework for a genetically structured model is presented. The framework allows description of the interactions in a system of regulatory and structural genes. The model assumes equilibrium kinetics for the binding of regulatory proteins to the promoter regions of the genes and includes the possible activati...

journal_title：Biotechnology and bioengineering

authors： Agger T,Nielsen J

更新日期：1999-01-01 00:00:00

abstract：:While feeding protocols and ectopic expression of anti-apoptotic genes have been used to improve the viability of hybridoma cell lines, the effect of the expression levels of survival genes on the behavior of hybridomas following nutrient supplementation is unknown. In this study, we compared the behavior of the Sp2/0...

journal_title：Biotechnology and bioengineering

authors： Charbonneau JR,Furtak T,Lefebvre J,Gauthier ER

更新日期：2003-02-05 00:00:00

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...

journal_title：Biotechnology and bioengineering

authors： Turner NA,Needs EC,Khan JA,Vulfson EN

更新日期：2001-01-05 00:00:00

abstract：:The enzymology and kinetics of tyrosine phenol lyase (TPL) from Erwinia herbicola, and tyrosine decarboxylase (TDC) from Streptococcus faecalis have been investigated for potential use in a coimmobilized multienzyme biocatalytic system for the production of dopamine. In this multienzyme biotransformation using whole c...

journal_title：Biotechnology and bioengineering

authors： Anderson WA,Moo-Young M,Legge RL

更新日期：1992-03-25 00:00:00

abstract：:Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...

journal_title：Biotechnology and bioengineering

authors： Rodrigues AF,Guerreiro MR,Santiago VM,Dalba C,Klatzmann D,Alves PM,Carrondo MJ,Coroadinha AS

更新日期：2011-11-01 00:00:00

abstract：:An approach is presented for the stable covalent immobilization of proteins with a high retention of biological activity. First, chemical modification studies were used to establish enzyme structural and functional properties relevant to the covalent immobilization of an enzyme to agarose based supports. Heparinase wa...

journal_title：Biotechnology and bioengineering

authors： Leckband D,Langer R

更新日期：1991-02-05 00:00:00

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

journal_title：Biotechnology and bioengineering

authors： Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

更新日期：1997-12-20 00:00:00

abstract：:A novel two-stage bioreactor has been designed for a combined submerged (SF) and solid substrate fermentation (SSF) of wheat straw. The straw was pretreated with steam, and cellulases from the culture fluid of Trichoderma reesei were adsorbed on it for increased bioconvertibility. SSF was conducted in the top part of ...

journal_title：Biotechnology and bioengineering

authors： Viesturs UE,Strikauska SV,Leite MP,Berzins AJ,Tengerdy RP

更新日期：1987-08-05 00:00:00

abstract：:A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

journal_title：Biotechnology and bioengineering

authors： Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

更新日期：2016-04-01 00:00:00

abstract：:The morphology of mycelial fungi in liquid culture effects culture rheology and this in turn may affect product yield. It is therefore important to understand how environmental factors influence mycelial morphology and this paper describes the effect of dilution rate on two strains of Fusarium graminearum, the relativ...

journal_title：Biotechnology and bioengineering

authors： Wiebe MG,Trinci AP

更新日期：1991-06-05 00:00:00

abstract：:Disulfide-rich peptides isolated from cone snails are of great interest as drug leads due to their high specificity and potency toward therapeutically relevant ion channels and receptors. They commonly contain the inhibitor cystine knot (ICK) motif comprising three disulfide bonds forming a knotted core. Here we repor...

journal_title：Biotechnology and bioengineering

authors： Kwon S,Bosmans F,Kaas Q,Cheneval O,Conibear AC,Rosengren KJ,Wang CK,Schroeder CI,Craik DJ

更新日期：2016-10-01 00:00:00

abstract：:Affinity chromatography using immobilized S-protein was used for the screening of affinity peptide ligands from two soluble peptide libraries. Peptide library I consisted of octamers with glycine (G) at both termini of each peptide, i.e. GXXXXXXG. The six center positions were constructed using random sequences of six...

journal_title：Biotechnology and bioengineering

authors： Huang PY,Carbonell RG

更新日期：1999-06-20 00:00:00

abstract：:A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a pr...

journal_title：Biotechnology and bioengineering

authors： Nie H,Soh BW,Fu YC,Wang CH

更新日期：2008-01-01 00:00:00

abstract：:Hyperacute rejection following xenogeneic transplantation in primates is mediated by naturally occurring IgM antibodies, which are specifically directed to alpha-Galactosyl residues on many nonprimate mammalian cells. Current approaches to remove these anti-alphaGal IgM include plasmapheresis followed by immunoaffinit...

journal_title：Biotechnology and bioengineering

authors： Karoor S,Molina J,Buchmann CR,Colton C,Logan JS,Henderson LW

更新日期：2003-01-20 00:00:00

abstract：:Protein localization in Saccharomyces cerevisiae was studied with two plasmid systems used as a model: one containing the SUC2 structural gene fused with the MFalpha1 (alpha-factor) promoter and signal-sequence, the other containing the entire SUC2 gene. Special emphasis was placed on the effect of promoter/signal-seq...

journal_title：Biotechnology and bioengineering

authors： Marten MR,Seo JH

更新日期：1989-11-01 00:00:00

abstract：:The results from this study demonstrate that neutral resins can selectively concentrate specific indole alkaloids from dilute aqueous mixtures. Adsorption was observed to provide a one to two order of magnitude improvement in concentrating these alkaloids, as compared to an equivalent single-staged extraction. Since t...

journal_title：Biotechnology and bioengineering

authors： Payne GF,Shuler ML

更新日期：1988-06-05 00:00:00

abstract：:Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ tran...

journal_title：Biotechnology and bioengineering

authors： Perez-Basterrechea M,Esteban MM,Vega JA,Obaya AJ

更新日期：2018-12-01 00:00:00

abstract：:This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

journal_title：Biotechnology and bioengineering

authors： Wang QQ,Zhu JY,Hunt CG,Zhan HY

更新日期：2012-08-01 00:00:00

abstract：:Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...

journal_title：Biotechnology and bioengineering

authors： Zhao L,Ye B,Zhang Q,Cheng D,Zhou C,Cheng S,Yan X

更新日期：2019-08-01 00:00:00

abstract：:A phenomenological model of biocide action against microbial biofilms was derived. Processes incorporated in the model include bulk flow in and out of a well-mixed reactor, transport of dissolved species into the biofilm, substrate consumption by bacterial metabolism, bacterial growth, advection of cell mass within th...

journal_title：Biotechnology and bioengineering

authors： Stewart PS,Hamilton MA,Goldstein BR,Schneider BT

更新日期：1996-02-20 00:00:00

abstract：:Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

journal_title：Biotechnology and bioengineering

authors： Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

更新日期：2006-02-20 00:00:00

abstract：:In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulations were performed to manipulate microparticles toward target areas. Base...

journal_title：Biotechnology and bioengineering

authors： Ino K,Shiku H,Ozawa F,Yasukawa T,Matsue T

更新日期：2009-11-01 00:00:00

abstract：:This study investigates the scaling of photobioreactor productivity based on the growth of Nannochloropsis salina incorporating the effects of direct and diffuse light. The scaling and optimization of photobioreactor geometry was analyzed by determining the growth response of a small-scale system designed to represent...

journal_title：Biotechnology and bioengineering

authors： Quinn JC,Turner CW,Bradley TH

更新日期：2012-02-01 00:00:00