Determination of the baculovirus transducing titer in mammalian cells.

abstract：:A bubble column (0.05 m(3)) and an air-lift fermentor (1.2 m(3)) were used for the production of the exocellular microbial polysaccharide xanthan with Xanthomonas campestris in a synthetic medium. Upon oxygen depletion in the liquid, the xanthan production rate dropped sharply and then became a linear function of the ...

journal_title：Biotechnology and bioengineering

authors： Suh IS,Schumpe A,Deckwer WD

更新日期：1992-01-05 00:00:00

abstract：:Start-up phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. In this study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment...

journal_title：Biotechnology and bioengineering

authors： Seoane J,Sin G,Lardon L,Gernaey KV,Smets BF

更新日期：2010-01-01 00:00:00

abstract：:Vascular leak is a key driver of organ injury in diseases, and strategies that reduce enhanced permeability and vascular inflammation are promising therapeutic targets. Activation of the angiopoietin-1 (ANG1)-Tie2 tyrosine kinase signaling pathway is an important regulator of vascular quiescence. Here we describe the ...

journal_title：Biotechnology and bioengineering

authors： Liu P,Ryczko M,Xie X,Baardsnes J,Lord-Dufour S,Duroche Y,Hicks EA,Taiyab A,Sheardown H,Quaggin SE,Jin J

更新日期：2021-01-01 00:00:00

abstract：:Both the forward and backward reactions of xylose isomerase (Sweetzyme Q) with xylose and glucose as substrates have been studied in terms of kinetics and thermodynamics. The relationship between the two reactions can thus be determined. Much attention has been given to the reaction with xylose as substrate. The optim...

journal_title：Biotechnology and bioengineering

authors： Olivier SP,du Toit PJ

更新日期：1986-05-01 00:00:00

abstract：:Poly lactic acid (PLA) based plastics is renewable, bio-based, and biodegradable. Although present day PLA is composed of mainly L-LA, an L- and D- LA copolymer is expected to improve the quality of PLA and expand its use. To increase the number of thermotolerant microbial biocatalysts that produce D-LA, a derivative ...

journal_title：Biotechnology and bioengineering

authors： Awasthi D,Wang L,Rhee MS,Wang Q,Chauliac D,Ingram LO,Shanmugam KT

更新日期：2018-02-01 00:00:00

abstract：:The denitrification capability of Cluster 1 Defluviicoccus vanus-related glycogen-accumulating organisms (DvGAOs) is investigated. A sequencing batch reactor (SBR) fed with acetate as the sole carbon source was operated under alternating anaerobic-aerobic conditions to enrich Cluster 1 DvGAOs. Fluorescence in situ hyb...

journal_title：Biotechnology and bioengineering

authors： Wang X,Zeng RJ,Dai Y,Peng Y,Yuan Z

更新日期：2008-04-15 00:00:00

abstract：:Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...

journal_title：Biotechnology and bioengineering

authors： Ghosh P,Lin M,Vogel JH,Choy D,Haynes C,von Lieres E

更新日期：2014-08-01 00:00:00

abstract：:Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

journal_title：Biotechnology and bioengineering

authors： Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

更新日期：2000-10-20 00:00:00

abstract：:The removal of substate molecules from aerobic microbial cultures is due to both consumption by microorganisms and stripping by the air stream. The air stripping component can be described by a constant parameter for low concentrations of volatile substrates. This air stripping parameter was found to have a value of 0...

journal_title：Biotechnology and bioengineering

authors： Singh N,Hill GA

更新日期：1987-09-01 00:00:00

abstract：:Mixed culture of microorganisms immobilized onto Celite diatomaceous earth particles were used to degrade 3,4-dichloroaniline (34DCA) in a three-phase draft tube fluidized bed bioreactor. Biodegradation was confirmed as the dominant removal mechanism by measurements of the concomitant chloride ion evolution. Degradati...

journal_title：Biotechnology and bioengineering

authors： Livingston AG

更新日期：1991-07-01 00:00:00

abstract：:Lectin-like function was demonstrated in this study for a novel water-soluble polymer with phenylboronic acid residues (poly (AAPBA-DMAm)), which induced appreciable proliferation of murine spleen lymphocytes with an increased expression of interleukin-2 (IL-2) receptor on their surface. Consequently, boosted prolifer...

journal_title：Biotechnology and bioengineering

authors： Uchimura E,Otsuka H,Okano T,Sakurai Y,Kataoka K

更新日期：2001-02-05 00:00:00

abstract：:Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...

journal_title：Biotechnology and bioengineering

authors： Li XM,Liang BS,Wang HY

更新日期：1988-02-20 00:00:00

abstract：:In this work the question was addressed if in nitrite-oxidizing activated sludge systems the environmental competition between Nitrobacter spp. and Nitrospira spp., which only recently has been discovered to play a role in these systems, is affected by the nitrite concentrations. Two parallel chemostats were inoculate...

journal_title：Biotechnology and bioengineering

authors： Nogueira R,Melo LF

更新日期：2006-09-05 00:00:00

abstract：:A cyclic fed-batch bioprocess is designed and a significant improvement of rice alpha-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media...

journal_title：Biotechnology and bioengineering

authors： Chang CC,Ryu DD,Park CS,Kim JY

更新日期：1998-08-05 00:00:00

abstract：:The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatment...

journal_title：Biotechnology and bioengineering

authors： Wang YF,Masuda M,Tsujimura S,Kano K

更新日期：2008-10-15 00:00:00

abstract：:Fungal biofilters have been recently studied as an alternative to the bacterial systems for the elimination of hydrophobic volatile organic compounds (VOC). Fungi foster reduced transport limitation of hydrophobic VOCs due to their hydrophobic surface and extended gas exchange area associated to the hyphal growth. Nev...

journal_title：Biotechnology and bioengineering

authors： Vergara-Fernández A,Hernández S,Revah S

更新日期：2011-04-01 00:00:00

abstract：:Metabolically active resting (i.e., nongrowing) bacterial cells have a high potential in cofactor-dependent redox biotransformations. Where growing cells require carbon and energy for biomass production, resting cells can potentially exploit their metabolism more efficiently for redox biocatalysis allowing higher spec...

journal_title：Biotechnology and bioengineering

authors： Julsing MK,Kuhn D,Schmid A,Bühler B

更新日期：2012-05-01 00:00:00

abstract：:C-terminal lysine (C-K) variants are commonly observed in therapeutic monoclonal antibodies and recombinant proteins. Heterogeneity of C-K residues is believed to result from varying degree of proteolysis by endogenous carboxypeptidase(s) during cell culture production. The achievement of batch-to-batch culture perfor...

journal_title：Biotechnology and bioengineering

authors： Luo J,Zhang J,Ren D,Tsai WL,Li F,Amanullah A,Hudson T

更新日期：2012-09-01 00:00:00

abstract：:The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol...

journal_title：Biotechnology and bioengineering

authors： Hannoun BJ,Stephanopoulos G

更新日期：1986-06-01 00:00:00

abstract：:The growth dynamics of a methanol utilizing bacterium, L3, in batch bioreactors were experimentally investigated. Formaldehyde, a key intermediate of methanol oxidation, is indicated to have a significant role in the complex batch growth behavior of L3. The intricate batch growth dynamics of many microorganisms can be...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Lim HC

更新日期：1984-11-01 00:00:00

abstract：:The results from this study demonstrate that neutral resins can selectively concentrate specific indole alkaloids from dilute aqueous mixtures. Adsorption was observed to provide a one to two order of magnitude improvement in concentrating these alkaloids, as compared to an equivalent single-staged extraction. Since t...

journal_title：Biotechnology and bioengineering

authors： Payne GF,Shuler ML

更新日期：1988-06-05 00:00:00

abstract：:A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...

journal_title：Biotechnology and bioengineering

authors： Senerovic L,Stankovic N,Spizzo P,Basso A,Gardossi L,Vasiljevic B,Ljubijankic G,Tisminetzky S,Degrassi G

更新日期：2006-02-05 00:00:00

abstract：:Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering ...

journal_title：Biotechnology and bioengineering

authors： Tschon M,Brogini S,Parrilli A,Bertoldi S,Silini A,Parolini O,Faré S,Martini L,Veronesi F,Fini M,Giavaresi G

更新日期：2021-01-01 00:00:00

abstract：:The key to achieving successful, reproducible results following the introduction of beneficial microbes into soil relies on the survival rate of the inoculated bacteria in a heterogeneous soil environment and hence an improved encapsulation method was developed. Owing to the constraints associated with the inoculum fo...

journal_title：Biotechnology and bioengineering

authors： Young CC,Rekha PD,Lai WA,Arun AB

更新日期：2006-09-05 00:00:00

abstract：:Metastatic disease remains one of the primary reasons for cancer-related deaths, yet the majority of in vitro cancer models focus on the primary tumor sites. Here, we describe a metastasis-on-a-chip device that houses multiple bioengineered three-dimensional (3D) organoids, established by a 3D photopatterning techniqu...

journal_title：Biotechnology and bioengineering

authors： Aleman J,Skardal A

更新日期：2019-04-01 00:00:00

abstract：:The spatial cell-material interaction remains vital issue in forming biodegradable scaffolds in Tissue Engineering. In this study, to understand the influence of spatial architecture on cellular behavior, 2D and 3D chitosan scaffolds of 50-190 kD and >310 kD MW were synthesized through air drying and controlled rate f...

journal_title：Biotechnology and bioengineering

authors： Huang Y,Siewe M,Madihally SV

更新日期：2006-01-05 00:00:00

abstract：:Myristic acid esterification has been performed by an immobilized lipase from Mucor Miehei both in n-hexane and in supercritical carbon dioxide (SCCO(2)). The enzyme is stable in SCCO(2) at 15 MPa and 323 K. The reaction rate is influenced by the concentration of water and by the reaction medium composition. A reactio...

journal_title：Biotechnology and bioengineering

authors： Dumont T,Barth D,Corbier C,Branlant G,Perrut M

更新日期：1992-06-20 00:00:00

abstract：:Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

journal_title：Biotechnology and bioengineering

authors： Kim Y,Arp DJ,Semprini L

更新日期：2002-12-05 00:00:00

abstract：:Mathematical models to describe transcription (Arnold et al. (2001); Biotech Bioeng 72:548-561) and translation (Mehra et al. (2003); Biotech Bioeng 84:822-841) in bacteria are modified in order to improve reaction kinetics and to include the number of polymerase molecules that are active on the DNA, as well as to inc...

journal_title：Biotechnology and bioengineering

authors： Kremling A

更新日期：2007-03-01 00:00:00

abstract：:Effective and efficient breakdown of lignocellulosic biomass remains a primary barrier for its use as a feedstock for renewable transportation fuels. A more detailed understanding of the material properties of biomass slurries during conversion is needed to design cost-effective conversion processes. A series of enzym...

journal_title：Biotechnology and bioengineering

authors： Roche CM,Dibble CJ,Knutsen JS,Stickel JJ,Liberatore MW

更新日期：2009-10-01 00:00:00