Dilution rate as a determinant of mycelial morphology in continuous culture.

abstract：:A model previously developed to characterize enzymatic in activation behavior was used to explain the non-first-order biphasic and grace period phenomena that are often observed with oligomeric enzymes. Luciferase and urease were used as model enzyme such as luciferase, the oligomer initially dissociates reversibly in...

journal_title：Biotechnology and bioengineering

authors： Lencki RW,Arul J,Neufeld RJ

更新日期：1992-12-20 00:00:00

abstract：:After injury to the central nervous system, a glial scar develops that physically and biochemically inhibits axon growth. In the scar, activated astrocytes secrete inhibitory extracellular matrix, of which chondroitin sulfate proteoglycans (CSPGs) are considered the major inhibitory component. An inhibitory interface ...

journal_title：Biotechnology and bioengineering

authors： Tuinstra HM,Ducommun MM,Briley WE,Shea LD

更新日期：2013-03-01 00:00:00

abstract：:In view of the biochemical reaction catalyzed by enzyme powder suspended in a water-insoluble organic solvent, an equation was derived to estimate the amount of water bound to the enzyme powder. With this equation, an apparent adsorption isotherm between free water (water freely dissolved in benzene) and bound water (...

journal_title：Biotechnology and bioengineering

authors： Yamane T,Kojima Y,Ichiryu T,Nagata M,Shimizu S

更新日期：1989-09-01 00:00:00

abstract：:Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...

journal_title：Biotechnology and bioengineering

authors： Choi MH,Xu J,Rho JK,Shim JH,Yoon SC

更新日期：2009-03-01 00:00:00

abstract：:The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (...

journal_title：Biotechnology and bioengineering

authors： Rossouw CL,Chetty A,Moolman FS,Birkholtz LM,Hoppe H,Mancama DT

更新日期：2012-08-01 00:00:00

abstract：:A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...

journal_title：Biotechnology and bioengineering

authors： Corchero JL,Villaverde A

更新日期：1998-06-20 00:00:00

abstract：:The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have established a high resol...

journal_title：Biotechnology and bioengineering

authors： Amrhein S,Bauer KC,Galm L,Hubbuch J

更新日期：2015-12-01 00:00:00

abstract：:The performance of an industrial pharmaceutical process (production of an active pharmaceutical ingredient by fermentation, API) was modeled by multiblock partial least squares (MBPLS). The most important process stages are inoculum production and API production fermentation. Thirty batches (runs) were produced accord...

journal_title：Biotechnology and bioengineering

authors： Lopes JA,Menezes JC,Westerhuis JA,Smilde AK

更新日期：2002-11-20 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:The relationship between oxygen concentration and growth rate in the yeast Trichosporon cutaneum was studied. In order to establish the conditions for purely oxygen-limited growth, the cells were first grown in a carbon-limited chemostat, and kinetic parameters determined. The cells were then grown in an oxygen-limite...

journal_title：Biotechnology and bioengineering

authors： Käppeli O,Fiechter A

更新日期：1982-11-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering th...

journal_title：Biotechnology and bioengineering

authors： Kiefer R,Jurisic M,Dahlem C,Koch M,Schmitt MJ,Kiemer AK,Schneider M,Breinig F

更新日期：2020-03-01 00:00:00

abstract：:Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondro...

journal_title：Biotechnology and bioengineering

authors： Oh TI,Kim C,Karki B,Son Y,Lee E,Woo EJ

更新日期：2015-02-01 00:00:00

abstract：:After separation by microscale non-denaturing two-dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after the spot of carboxylesterase is e...

journal_title：Biotechnology and bioengineering

authors： Shimazaki Y,Miyamoto M

更新日期：2007-11-01 00:00:00

abstract：:Bioaffinity interactions have been, and continue to be, successfully adapted from nature for use in separation and detection applications. It has been previously reported that the magnetophoretic mobility of labeled cells show a saturation type phenomenon as a function of the concentration of the free antibody-magneti...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Williams PS,Zborowski M,Chalmers JJ

更新日期：2006-12-05 00:00:00

abstract：:Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity...

journal_title：Biotechnology and bioengineering

authors： Yuan Y,He H,Lee LJ

更新日期：2009-02-15 00:00:00

abstract：:Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...

journal_title：Biotechnology and bioengineering

authors： Plimpton SR,Gołkowski M,Mitchell DG,Austin C,Eaton SS,Eaton GR,Gołkowski C,Voskuil M

更新日期：2013-07-01 00:00:00

abstract：:Enzymatic acrylation is a process of potentially strong interest to the chemical industry. Direct esterification involving acrylic acid is unfortunately rather slow, with inhibition phenomena appearing at high acid concentrations. In the present study the acrylation of 1-octanol catalyzed by immobilized Candida antarc...

journal_title：Biotechnology and bioengineering

authors： Nordblad M,Adlercreutz P

更新日期：2008-04-15 00:00:00

abstract：:A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...

journal_title：Biotechnology and bioengineering

authors： Rozzi A,Castellazzi L,Speece RE

更新日期：2002-01-05 00:00:00

abstract：:An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recomb...

journal_title：Biotechnology and bioengineering

authors： Lindsay DA,Betenbaugh MJ

更新日期：1992-03-15 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00

abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...

journal_title：Biotechnology and bioengineering

authors： Bryers JD

更新日期：1985-05-01 00:00:00

abstract：:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

journal_title：Biotechnology and bioengineering

authors： van der Pol L,Bakker WA,Tramper J

更新日期：1992-06-05 00:00:00

abstract：:The heterogeneous nature of hollow fiber reactors for cell cultivation requires special considerations for proper design and operation. Downstream concentration of high-molecular-weight proteins has been measured in the shell side of ultrafiltration hollow fiber bioreactors. This distribution resulted from shell-side ...

journal_title：Biotechnology and bioengineering

authors： Piret JM,Cooney CL

更新日期：1990-11-01 00:00:00

abstract：:The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, field-flow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed...

journal_title：Biotechnology and bioengineering

authors： Tamura A,Fukutani Y,Takami T,Fujii M,Nakaguchi Y,Murakami Y,Noguchi K,Yohda M,Odaka M

更新日期：2015-01-01 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...

journal_title：Biotechnology and bioengineering

authors： Taipa MA,Cabral JM,Santos H

更新日期：1993-03-15 00:00:00

abstract：:Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this stud...

journal_title：Biotechnology and bioengineering

authors： Baek K,Yu J,Jeong J,Sim SJ,Bae S,Jin E

更新日期：2018-03-01 00:00:00

abstract：:A method for purifying alpha-amylase inhibitor from wheat meal based on immobilized metal affinity with a thermosensitive copolymer is developed. The studies represent the thermoprecipitation properties of the copolymers of N-isopropylacrylamide (NIPAM) with iminodiacetic acid (IDA) and 1-vinylimidazole (VI), respecti...

journal_title：Biotechnology and bioengineering

authors： Kumar A,Galaev IY,Mattiasson B

更新日期：1998-09-20 00:00:00

abstract：:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...

journal_title：Biotechnology and bioengineering

authors： Park JB,Clark DS

更新日期：2006-05-05 00:00:00

abstract：:The rate of diffusion of serum albumin (MW 6.9 x 10(4) D) out of beads of calcium alginate gels depends upon the concentration and uronic acid composition of the alginate (ManA/GulA ratio), the conditions under which the beads are produced, the pH, and the temperature. The diffusion coefficient decreases with increasi...

journal_title：Biotechnology and bioengineering

authors： Martinsen A,Storrø I,Skjårk-Braek G

更新日期：1992-01-20 00:00:00