Cyclodextrins micrometric powders obtained by supercritical fluid processing.

abstract：:Currently in America the biofuel ethanol is primarily being produced by the dry grind technique to obtain the starch contained in the corn grains and subsequently subjected to fermentation. This so-called 1st generation technology has two setbacks; first the lingering debate whether its life cycle contributes to a red...

journal_title：Biotechnology and bioengineering

authors： Brehmer B,Bals B,Sanders J,Dale B

更新日期：2008-09-01 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:Benzil was reduced stereospecifically to (S)-benzoin by Bacillus cereus strain Tim-r01. To isolate the gene responsible for asymmetric reduction, we constructed a library consisting of Escherichia coli clones that harbored plasmids expressing Bacillus cereus genes. The library was screened using the halo formation ass...

journal_title：Biotechnology and bioengineering

authors： Maruyama R,Nishizawa M,Itoi Y,Ito S,Inoue M

更新日期：2001-12-20 00:00:00

abstract：:The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponen...

journal_title：Biotechnology and bioengineering

authors： Zheng ZM,Xu YZ,Liu HJ,Guo NN,Cai ZZ,Liu DH

更新日期：2008-08-01 00:00:00

abstract：:Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

journal_title：Biotechnology and bioengineering

authors： Vemuri GN,Eiteman MA,Altman E

更新日期：2006-06-20 00:00:00

abstract：:We present a mathematical model which includes TNF-alpha initiated survival and apoptotic cascades, as well as nuclear transcription of IkappaB. These pathways play a crucial role in deciding cell fate in response to inflammation and infection. Our model incorporates known specific protein-protein interactions as iden...

journal_title：Biotechnology and bioengineering

authors： Rangamani P,Sirovich L

更新日期：2007-08-01 00:00:00

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:Protein localization in Saccharomyces cerevisiae was studied with two plasmid systems used as a model: one containing the SUC2 structural gene fused with the MFalpha1 (alpha-factor) promoter and signal-sequence, the other containing the entire SUC2 gene. Special emphasis was placed on the effect of promoter/signal-seq...

journal_title：Biotechnology and bioengineering

authors： Marten MR,Seo JH

更新日期：1989-11-01 00:00:00

abstract：:The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, field-flow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed...

journal_title：Biotechnology and bioengineering

authors： Tamura A,Fukutani Y,Takami T,Fujii M,Nakaguchi Y,Murakami Y,Noguchi K,Yohda M,Odaka M

更新日期：2015-01-01 00:00:00

abstract：:Steady-state simulations using our previously developed structured kinetic model of antibody synthesis and secretion by hybridoma cells are used here in conjunction with factorial design analysis to identify intracellular parameters important in determining the specific antibody secretion rate and predict the dependen...

journal_title：Biotechnology and bioengineering

authors： Bibila TA,Flickinger MC

更新日期：1992-02-05 00:00:00

abstract：:Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...

journal_title：Biotechnology and bioengineering

authors： Gorman LA,Dordick JS

更新日期：1992-02-20 00:00:00

abstract：:Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to contribute to the ...

journal_title：Biotechnology and bioengineering

authors： Grossmann C,Tintinger R,Zhu J,Maurer G

更新日期：1998-12-20 00:00:00

abstract：:Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...

journal_title：Biotechnology and bioengineering

authors： Yu B,Chang TM

更新日期：2004-06-30 00:00:00

abstract：:The biological molecule responsible for the suppression of pyritic sulfur in fine coal simulated froth flotation treated with bacteria was identified. Protein was found to be the most effective agent in pyrite suppression of the three cell components (protein, lipid, and carbohydrate) assayed. Coal recovery and ash re...

journal_title：Biotechnology and bioengineering

authors： Stainthorpe AC

更新日期：1989-02-05 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:Metabolite accumulation has pleiotropic, toxic, or beneficial effects on cell physiology, but such effects are not well understood at the molecular level. Cells respond and adapt to metabolite stress by mechanisms largely unexplored, especially in the context of multiple and simultaneous stresses. Solventogenic and re...

journal_title：Biotechnology and bioengineering

authors： Alsaker KV,Paredes C,Papoutsakis ET

更新日期：2010-04-15 00:00:00

abstract：:During continuous lipase-catalyzed oleic acid esterification by ethanol in n-hexane, the oleic acid conversion, initially at 95%, decreases to 20% after 2 h. This decrease is caused by the accumulation of the water produced in the course of the reaction in the packed-bed reactor (PBR). In order to improve the PBR effi...

journal_title：Biotechnology and bioengineering

authors： Colombié S,Tweddell RJ,Condoret JS,Marty A

更新日期：1998-11-05 00:00:00

abstract：:We performed a macrokinetic and quantitative microbial investigation of a continuously operating bench-scale biofilter treating styrene-polluted gases. The device was filled with a mixture of peat and glass beads as packing medium and inoculated with the styrene-oxidizing strain, Rhodococcus rhodochrous AL NCIMB 13259...

journal_title：Biotechnology and bioengineering

authors： Zilli M,Converti A,Di Felice R

更新日期：2003-07-05 00:00:00

abstract：:Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale-up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model ...

journal_title：Biotechnology and bioengineering

authors： Souret FF,Kim Y,Wyslouzil BE,Wobbe KK,Weathers PJ

更新日期：2003-09-20 00:00:00

abstract：:Morphology has a crucial effect on productivity and the supply of substrate for cultures of filamentous fungi. However, cultivation parameters leading to the desired morphology are often chosen empirically as the mechanisms governing the processes involved are usually unknown. For coagulating microorganisms like Asper...

journal_title：Biotechnology and bioengineering

authors： Grimm LH,Kelly S,Hengstler J,Göbel A,Krull R,Hempel DC

更新日期：2004-07-20 00:00:00

abstract：:Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cyt...

journal_title：Biotechnology and bioengineering

authors： Prudhomme WA,Duggar KH,Lauffenburger DA

更新日期：2004-11-05 00:00:00

abstract：:Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

journal_title：Biotechnology and bioengineering

authors： Davidson AJ,Ellis MJ,Chaudhuri JB

更新日期：2010-08-15 00:00:00

abstract：:We report the first direct images of the hydration of protein powders. Using an environmental scanning electron microscope (ESEM) we have taken a series of micrographs of a region of the enzyme (subtilisin) power whilst hydrating the sample. In addition, the sample has been viewed during exposure to toluene vapors. Th...

journal_title：Biotechnology and bioengineering

authors： Roziewski K,Russell AJ

更新日期：1992-05-01 00:00:00

abstract：:A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1992-10-20 00:00:00

abstract：:Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m(3) occurred at pH 4.0 and a dilution rate of 0.0391 h(-1) where residual substrate was 0.43 kg/m(3), but no cellulase wa...

journal_title：Biotechnology and bioengineering

authors： Schafner DW,Toledo RT

更新日期：1992-04-05 00:00:00

abstract：:We have used a high-throughput LC/MS/UV/ELSD method to rapidly determine the absolute quantity and purity of 42 organic compounds from seven lead discovery libraries. A general calibration curve generated from a different set of 42 compounds with seven different scaffolds was used in this analysis. We have also studie...

journal_title：Biotechnology and bioengineering

authors： Fang L,Pan J,Yan B

更新日期：2000-01-01 00:00:00

abstract：:Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...

journal_title：Biotechnology and bioengineering

authors： Woodley JM,Brazier AJ,Lilly MD

更新日期：1991-01-20 00:00:00

abstract：:To investigate the effects of factors secreted by different cell lines on human monoclonal antibody (MAb) integrity, 600 mg of a human MAb, which specifically binds to human erythrocytes, were produced in a perfusion process. After purification by protein A affinity chromatography, the MAb was used for integrity testi...

journal_title：Biotechnology and bioengineering

authors： Ackermann M,Jäger V,Marx U

更新日期：1995-01-20 00:00:00

abstract：:Polypeptides containing between 4 and 32 repeats of a resilin-inspired sequence AQTPSSYGAP, derived from the mosquito Anopheles gambiae, have been used as tags on recombinant fusion proteins. These repeating polypeptides were inspired by the repeating structures that are found in resilins and sequence-related proteins...

journal_title：Biotechnology and bioengineering

authors： Lyons RE,Elvin CM,Taylor K,Lekieffre N,Ramshaw JA

更新日期：2012-12-01 00:00:00

abstract：:The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glut...

journal_title：Biotechnology and bioengineering

authors： Miller WM,Wilke CR,Blanch HW

更新日期：1989-01-20 00:00:00