Abstract:
:Benzil was reduced stereospecifically to (S)-benzoin by Bacillus cereus strain Tim-r01. To isolate the gene responsible for asymmetric reduction, we constructed a library consisting of Escherichia coli clones that harbored plasmids expressing Bacillus cereus genes. The library was screened using the halo formation assay, and one clone showed benzil reduction to (S)-benzoin. Thus, this clone seemed to carry a plasmid encoding a Bacillus cereus benzil reductase. The deduced amino acid sequence had marked homologies to the Bacillus subtilis yueD protein (41% identity), the yeast open reading frame YIR036C protein (31%), and the mammalian sepiapterin reductases (28% to 30%), suggesting that benzil reductase is a novel short-chain de-hydrogenases/ reductase.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Maruyama R,Nishizawa M,Itoi Y,Ito S,Inoue Mdoi
10.1002/bit.1191subject
Has Abstractpub_date
2001-12-20 00:00:00pages
630-3issue
6eissn
0006-3592issn
1097-0290pii
10.1002/bit.1191journal_volume
75pub_type
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