Use of a tapered fluidized bed as a continuous bioreactor.

abstract：:Measurements of k(L)a were carried out in 1. 5- and 5-L New Brunswick Scientific CelliGen bioreactors. The measured k(L)a in water were identical for both vessel sizes operated in similar condition. The mass transfer rate increased with temperature, mixing speed, and aeration rate, with this last parameter being the m...

journal_title：Biotechnology and bioengineering

authors： Johnson M,André G,Chavarie C,Archambault J

更新日期：1990-01-05 00:00:00

abstract：:A new method is presented to precipitate proteins and amino acids from reverse micelles by dehydrating the micelles with molecular sieves. Nearly complete precipitation is demonstrated for alpha-chymotrypsin, cytochromec, and trytophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isooctane/water reverse micelle solut...

journal_title：Biotechnology and bioengineering

authors： Gupta RB,Han CJ,Johnston KP

更新日期：1994-09-20 00:00:00

abstract：:Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...

journal_title：Biotechnology and bioengineering

authors： Gupta KG,Yadav NK,Dhawan S

更新日期：1978-12-01 00:00:00

abstract：:The kinetic properties of acetate-degrading methanogenic granular sludge of different mean diameters were assessed at different up-flow velocities (V(up)). Using this approach, the influence of internal and external mass transfer could be estimated. First, the apparent Monod constant (K(S)) for each data set was calcu...

journal_title：Biotechnology and bioengineering

authors： Gonzalez-Gil G,Seghezzo L,Lettinga G,Kleerebezem R

更新日期：2001-04-20 00:00:00

abstract：:Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

journal_title：Biotechnology and bioengineering

authors： Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

更新日期：2019-01-01 00:00:00

abstract：:The naturally existing microbial hosts can rarely satisfy industrial requirements, thus there has always been an intense effort in strain engineering to meet the needs of these bioprocesses. Here, in this work, we want to prove the concept that engineering global transcription factor cAMP receptor protein (CRP) of Esc...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Chong H,Ching CB,Jiang R

更新日期：2012-05-01 00:00:00

abstract：:Flow cytometry is a fast and sensitive method that allows monitoring of different cellular parameters on large samples of a population. Protein distributions give relevant information on growth dynamics, since they are related to the age distribution and depend on the law of growth of the population and the law of pro...

journal_title：Biotechnology and bioengineering

authors： Alberghina L,Mariani L,Martegani E,Vanoni M

更新日期：1983-05-01 00:00:00

abstract：:Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and...

journal_title：Biotechnology and bioengineering

authors： Rudolph G,Lindner P,Gierse A,Bluma A,Martinez G,Hitzmann B,Scheper T

更新日期：2008-01-01 00:00:00

abstract：:Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...

journal_title：Biotechnology and bioengineering

authors： Skardal A,Devarasetty M,Forsythe S,Atala A,Soker S

更新日期：2016-09-01 00:00:00

abstract：:In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...

journal_title：Biotechnology and bioengineering

authors： Ottengraf SP,Van Den Oever AH

更新日期：1983-12-01 00:00:00

abstract：:The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...

journal_title：Biotechnology and bioengineering

authors： Yuan H,Liu Y,Li J,Shin HD,Du G,Shi Z,Chen J,Liu L

更新日期：2018-09-01 00:00:00

abstract：:The long hydrocarbon fatty acyl chain is energy rich, making it an ideal precursor for liquid transportation fuels and high-value oleo chemicals. As Saccharomyces cerevisiae has many advantages for industrial production compared to Escherichia coli. Here, we attempted to engineer Saccharomyces cerevisiae for overprodu...

journal_title：Biotechnology and bioengineering

authors： Li X,Guo D,Cheng Y,Zhu F,Deng Z,Liu T

更新日期：2014-09-01 00:00:00

abstract：:A modified mixing plane approach for steady state simulation of flow field in fully baffled biological reactor is presented and discussed. Without requiring any experimental input, this approach of dividing the vessel into suitable number of connected and disconnected zones; solving steady state equation separately in...

journal_title：Biotechnology and bioengineering

authors： Dubey H,Das SK,Panda T

更新日期：2006-11-05 00:00:00

abstract：:Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...

journal_title：Biotechnology and bioengineering

authors： Peyton SR,Kalcioglu ZI,Cohen JC,Runkle AP,Van Vliet KJ,Lauffenburger DA,Griffith LG

更新日期：2011-05-01 00:00:00

abstract：:Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...

journal_title：Biotechnology and bioengineering

authors： Criddle CS

更新日期：1993-05-01 00:00:00

abstract：:Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three-dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli on cancer cell metastasis, proliferation, and chemoresistance. To investigate the roles of shear...

journal_title：Biotechnology and bioengineering

authors： Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

更新日期：2019-11-01 00:00:00

abstract：:Polymeric membranes are increasingly used as supports for the immobilization of enzymes in bioreactors. One of the more common reactor types employed in lipase-catalyzed hydrolysis of oils, contains modified cellulose as a membrane material. We found that this type of material is readily attacked by cellulase present ...

journal_title：Biotechnology and bioengineering

authors： Derksen JT,Boswinkel G,Cuperus FP

更新日期：1992-10-05 00:00:00

abstract：:Accurate measurement of enzymatic cellulose digestibility (X) is important in evaluating the efficiency of lignocellulose pretreatment technologies, assessing the performance of reconstituted cellulase mixtures, and conducting economic analysis for biorefinery processes. We analyzed the effect of sugars contained in e...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Schell DJ,McMillan JD

更新日期：2007-01-01 00:00:00

abstract：:An analysis of the radial flow cell is presented to show that the assumption of creeping laminar flow should be used cautiously. Simple models which account for the influence of fluid inertial forces over most of the width of the plate are reviewed. A modified Reynolds number is introduced which may be used to test th...

journal_title：Biotechnology and bioengineering

authors： Fryer PJ,Slater NK,Duddridge JE

更新日期：1985-04-01 00:00:00

abstract：:The utilization and conversion of D-xylose, D-xylulose, L-arabinose, and xylitol by yeast strains have been investigated with the following results: (1) The majority of yeasts tested utilize D-xylose and produce polyols, ethanol, and organic acids. The type and amount of products formed varies with the yeast strains u...

journal_title：Biotechnology and bioengineering

authors： Gong CS,Claypool TA,McCracken LD,Maun CM,Ueng PP,Tsao GT

更新日期：1983-01-01 00:00:00

abstract：:This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-co...

journal_title：Biotechnology and bioengineering

authors： Pihlajaniemi V,Sipponen MH,Pastinen O,Nyyssölä A,Laakso S

更新日期：2016-12-01 00:00:00

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

journal_title：Biotechnology and bioengineering

authors： Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

更新日期：2006-02-20 00:00:00

abstract：:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...

journal_title：Biotechnology and bioengineering

authors： López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano G

更新日期：2017-03-01 00:00:00

abstract：:A starvation-based dissolved oxygen (DO) transient controller was developed to supply growth-limiting substrate to high cell density fed-batch cultures of recombinant Escherichia coli. The algorithm adjusted a preexisting feed rate in proportion to the culture's oxygen demand, which was estimated from transients in th...

journal_title：Biotechnology and bioengineering

authors： Whiffin VS,Cooney MJ,Cord-Ruwisch R

更新日期：2004-02-20 00:00:00

abstract：:The application of the unscented Kalman filter to control starvation-induced programmed cell death-apoptosis-in Chinese hamster ovary cells was investigated. Neural network-based sensitivity analysis identified glutamine and asparagine as two major amino acids that play a key role in the suppression of apoptosis. Dyna...

journal_title：Biotechnology and bioengineering

authors： Simon L,Karim MN

更新日期：2002-06-20 00:00:00

abstract：:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

journal_title：Biotechnology and bioengineering

authors： Schinkel H,Jacobs P,Schillberg S,Wehner M

更新日期：2008-01-01 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:A microarray targeting four sequenced strains in the Dehalococcoides (Dhc) genus was used to analyze gene expression in a robust long-term trichloroethene (TCE)-degrading microbial community (designated ANAS) during feeding cycles that involve conditions of periodic substrate supply. The Dhc transcriptome was examined...

journal_title：Biotechnology and bioengineering

authors： West KA,Lee PK,Johnson DR,Zinder SH,Alvarez-Cohen L

更新日期：2013-05-01 00:00:00

abstract：:The ability to capture cell-free DNA from the gastrointestinal tract, in a minimally invasive manner, could enhance our ability to diagnose gastrointestinal disease, or gain a better understanding of the spatial mapping of the intestinal microbiota. We, therefore, sought to identify a class of capture agents that coul...

journal_title：Biotechnology and bioengineering

authors： Hilmer AJ,Jeffrey RB,Park WG,Khosla C

更新日期：2017-04-01 00:00:00