Numerical simulation of a fully baffled biological reactor: the differential circumferential averaging mixing plane approach.


:A modified mixing plane approach for steady state simulation of flow field in fully baffled biological reactor is presented and discussed. Without requiring any experimental input, this approach of dividing the vessel into suitable number of connected and disconnected zones; solving steady state equation separately in each zone and then transferring information between them, provides a computationally less intensive alternative for simulating the flow in the whole vessel. Impeller used is the standard Rushton Turbine positioned at mid-height of the reactor and simulations are carried out using standard k-epsilon turbulence model implemented in CFD code FLUENT. Meshing is done using tetrahedral elements such that mesh size gradually increases from the center to the periphery. Most of the previous simulation works present only a few aspects of the flow field with scant importance to the energy balance in the tank and near tip turbulence. In this work, complete model prediction for velocity field and turbulence parameters (near tip and in the bulk region) are validated by comparison with experimental data. As compared to previous simulation works, the results predicted by this "Differential circumferential averaging mixing plane approach" show a better qualitative and quantitative agreement with the published experimental data. A distribution of energy dissipation in various zones of vessel is presented. Also a qualitative picture of flow field and stagnant zones inside the reactor is presented and discussed. Comparison of flow characteristics for different number of baffles shows that for the present dimension of the vessel, five baffles gives maximum enhanced mixing.


Biotechnol Bioeng


Dubey H,Das SK,Panda T




Has Abstract


2006-11-05 00:00:00












  • The kinetics of protein precipitation by different reagents.

    abstract::The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size wit...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chan MY,Hoare M,Dunnill P

    更新日期:1986-03-01 00:00:00

  • Determination of the baculovirus transducing titer in mammalian cells.

    abstract::Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

    更新日期:2006-02-20 00:00:00

  • Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme.

    abstract::Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bhargava S,Wenger KS,Marten MR

    更新日期:2003-04-05 00:00:00

  • Reference and counter electrode positions affect electrochemical characterization of bioanodes in different bioelectrochemical systems.

    abstract::The placement of the reference electrode (RE) in various bioelectrochemical systems is often varied to accommodate different reactor configurations. While the effect of the RE placement is well understood from a strictly electrochemistry perspective, there are impacts on exoelectrogenic biofilms in engineered systems ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang F,Liu J,Ivanov I,Hatzell MC,Yang W,Ahn Y,Logan BE

    更新日期:2014-10-01 00:00:00

  • Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency.

    abstract::In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

    更新日期:2020-01-01 00:00:00

  • Use of glucose-responsive material to regulate insulin release from constitutively secreting cells.

    abstract::Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cheng SY,Constantinidis I,Sambanis A

    更新日期:2006-04-20 00:00:00

  • Recovery of insect cells using hollow fiber microfiltration.

    abstract::An efficient method was developed for media separation and cell collection for eukaryotic cells growing in suspension. The method is based on tangential flow microfiltration using an open channel arrangement in a hollow fiber configuration. Best results (highest processing flux rate) for polysulfone hollow fibers were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trinh L,Shiloach J

    更新日期:1995-11-20 00:00:00

  • Laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613.

    abstract::Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gupta KG,Yadav NK,Dhawan S

    更新日期:1978-12-01 00:00:00

  • NADH and flavin fluorescence responses of starved yeast cultures to substrate additions.

    abstract::Model experiments were performed with starved yeast (Saccharomyces cerevisiae) cultures in a batch reactor in order to develop a better understanding of NAD(P)H and flavin culture fluorescence. Fluorescence was monitored during aerobic-anaerobic-aerobic transitions and ethanol and glucose substrate addition experiment...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Siano SA,Mutharasan R

    更新日期:1989-08-20 00:00:00

  • Physical modeling of animal cell damage by hydrodynamic forces in suspension cultures.

    abstract::Physical damage of animal cells in suspension culture, due to stirring and sparging, is coupled with complex metabolic responses. Nylon microcapsules, therefore, were used as a physical model to study the mechanisms of damage in a stirred bioreactor and in a bubble column. Microcapsule breaskage folowed first-order ki...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lu GZ,Gray MR,Thompson BG

    更新日期:1992-12-05 00:00:00

  • Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium spirulina platensis.

    abstract::The photosynthetic performance of a helical tubular photobioreactor ("Biocoil"), incorporating the filamentous cyanobacterium Spirulina platensis, was investigated. The photobioreactor was constructed in a cylindrical shape (0.9 m high) with a 0.25-m(2)basal area and a photostage comprising 60 m of transparent PVC tub...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Watanabe Y,de la Noüe J,Hall DO

    更新日期:1995-07-20 00:00:00

  • Production of recombinant proteins using multiple-copy gene integration in high-cell-density fermentations of Ralstonia eutropha.

    abstract::We have previously reported the development of a novel protein expression system based on Ralstonia eutropha. In this study we report on the influence of gene copynumber on recombinant protein expression in R. eutropha. We compare recombinant gene stability and expression levels of chromosomal integration with a plasm...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Srinivasan S,Barnard GC,Gerngross TU

    更新日期:2003-10-05 00:00:00

  • A theoretical method to improve and optimize the design of bioartificial livers.

    abstract::Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Davidson AJ,Ellis MJ,Chaudhuri JB

    更新日期:2010-08-15 00:00:00

  • Mitigation of ammonia inhibition by internal dilution in high-rate anaerobic digestion of food waste leachate and evidences of microbial community response.

    abstract::A high-rate anaerobic digestion of food waste leachate were tested using intermittent continuously stirred tank reactors (iCSTRs) to evaluate how severe ammonia inhibition could be mitigated with internal dilution strategy, and to identify how bacterial and archaeal community respond in genus and species level. Experi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yun YM,Kim DH,Cho SK,Shin HS,Jung KW,Kim HW

    更新日期:2016-09-01 00:00:00

  • Maximum production strategy for biodegradable copolymer P(HB-co-HV) in fed-batch culture of Alcaligenes eutrophus.

    abstract::A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shimizu H,Kozaki Y,Kodama H,Shioya S

    更新日期:1999-03-05 00:00:00

  • A synthetic O2 -tolerant butanol pathway exploiting native fatty acid biosynthesis in Escherichia coli.

    abstract::Several synthetic metabolic pathways for butanol synthesis have been reported in Escherichia coli by modification of the native CoA-dependent pathway from selected Clostridium species. These pathways are all dependent on the O2 -sensitive AdhE2 enzyme from Clostridium acetobutylicum that catalyzes the sequential reduc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pásztor A,Kallio P,Malatinszky D,Akhtar MK,Jones PR

    更新日期:2015-01-01 00:00:00

  • Halophilic archaea and their potential to generate renewable fuels and chemicals.

    abstract::Lignocellulosic biofuels and chemicals have great potential to reduce our dependence on fossil fuels and mitigate air pollution by cutting down on greenhouse gas emissions. Chemical, thermal, and enzymatic processes are used to release the sugars from the lignocellulosic biomass for conversion to biofuels. These proce...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Kasirajan L,Maupin-Furlow JA

    更新日期:2020-11-26 00:00:00

  • Fluorophore-labeled carbohydrate analysis of immunoglobulin fusion proteins: Correlation of oligosaccharide content with in vivo clearance profile.

    abstract::CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Flesher AR,Marzowski J,Wang WC,Raff HV

    更新日期:1995-06-05 00:00:00

  • Production of micronic particles of biocompatible polymer using supercritical carbon dioxide.

    abstract::Three micronization techniques, based on the use of supercritical carbon dioxide, were investigated to produce microspheres of a natural biocompatible polysaccharide. Particles smaller than 20 mum were obtained by means of the rapid expansion of a supercritical solution method (RESS), both with and without cosolvents....

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Benedetti L,Bertucco A,Pallado P

    更新日期:1997-01-20 00:00:00

  • TGF-beta1 regulation in hepatocyte-NIH3T3 co-culture is important for the enhanced hepatocyte function in 3D microenvironment.

    abstract::Co-culture of hepatocytes or hepatocyte spheroids with the supporting NIH3T3 in a 3D microcapsule formed with a hybrid natural/synthetic matrix has led to enhanced hepatocyte functions. We investigated the mechanism of the functional enhancement in co-culture with respect to the contributions of soluble factors and di...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chia SM,Lin PC,Yu H

    更新日期:2005-03-05 00:00:00

  • A new nodavirus-negative Trichoplusia ni cell line for baculovirus-mediated protein production.

    abstract::Cell lines derived from Trichoplusia ni (Tn) are widely used as hosts in the baculovirus-insect cell system (BICS). One advantage of Tn cell lines is they can produce recombinant proteins at higher levels than cell lines derived from other insects. However, Tn cell lines are persistently infected with an alphanodaviru...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Maghodia AB,Geisler C,Jarvis DL

    更新日期:2020-07-14 00:00:00

  • Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells.

    abstract::In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: van der Pol L,Bakker WA,Tramper J

    更新日期:1992-06-05 00:00:00

  • Protein interaction affinity determination by quantitative FRET technology.

    abstract::The dissociation constant, K(d) , is an important parameter for characterizing protein-protein interaction affinities. SUMOylation is one of the important protein post-translational modifications and it involves a multi-step enzymatic cascade reaction, resulting in peptide activation and substrate conjugation. Multipl...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Song Y,Rodgers VG,Schultz JS,Liao J

    更新日期:2012-11-01 00:00:00

  • Combination drug delivery via multilamellar vesicles enables targeting of tumor cells and tumor vasculature.

    abstract::Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

    更新日期:2018-06-01 00:00:00

  • Extractive acetonobutylic fermentation by coupling ultrafiltration and distillation.

    abstract::An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Minier M,Grateloup R,Blanc-Ferras E,Goma G

    更新日期:1990-04-15 00:00:00

  • Elucidation of enzyme control mechanisms using macroscopic measurements in a mixed substrate fermentation system.

    abstract::The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pih N,De Bernardez E,Dhurjati P

    更新日期:1988-03-01 00:00:00

  • A new extant respirometric assay to estimate intrinsic growth parameters applied to study plasmid metabolic burden.

    abstract::Start-up phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. In this study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Seoane J,Sin G,Lardon L,Gernaey KV,Smets BF

    更新日期:2010-01-01 00:00:00

  • Characterization of a three bacteria mixed culture in a chemostat: evaluation and application of a quantitative terminal-restriction fragment length polymorphism (T-RFLP) analysis for absolute and species specific cell enumeration.

    abstract::Growth dynamics of Pseudomonas aeruginosa, Burkholderia cepacia, and Staphylococcus aureus in a batch and chemostat, were investigated as a laboratory model system for persistent infections in cystic fibrosis. Most species-specific enumeration methods for mixed cultures are laborious or only qualitative, and therefore...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schmidt JK,König B,Reichl U

    更新日期:2007-03-01 00:00:00

  • Effects of short elastin-like peptides on filamentous particles and their transition behavior.

    abstract::While elastin-like polypeptides and peptides (ELPs) have been used for various stimulus-responsive applications, details of their switching remain unclear. We therefore constructed a novel series of filamentous phage particles displaying a high surface density of short ELPs. The surface display of ELPs did not disrupt...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hathorne AP,Bermudez H

    更新日期:2013-07-01 00:00:00

  • Pilot trials of the microbial degradation of Christos-Bitas water in oil emulsion (chocolate mousse) and BP llandarcy gas oil using venturi aeration.

    abstract::Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and v...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Berwick PG

    更新日期:1985-01-01 00:00:00