Abstract:
:In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to supply them with inorganic nutrients. Most organic compounds are oxidized to carbon dioxide and water. The compositions of the solid phase and the viable organisms present are such that aging is prevented, as a result of which a relatively high activity can be maintained during a long period of time (years). Experiments have been carried out in laboratory-scale columns with composite gas mixtures at varied concentrations and superficial gas velocities. The (macro) kinetics of the elimination processes have been studied, which enables the prediction of the elimination capacity of the filter bed.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Ottengraf SP,Van Den Oever AHdoi
10.1002/bit.260251222subject
Has Abstractpub_date
1983-12-01 00:00:00pages
3089-102issue
12eissn
0006-3592issn
1097-0290journal_volume
25pub_type
杂志文章abstract::The hollow fiber enzyme reactor with pulsation developed by Kim and Chang (1983) was operated in a differential mode by recycling a substrate solution, in order to assess the efficiency of ultrafiltration swing. The rates of lactose conversion by beta-galactosidase contained in the shell side of the reactor were measu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270813
更新日期:1985-08-01 00:00:00
abstract::The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000105)67:1<97::aid-bit1
更新日期:2000-01-05 00:00:00
abstract::The rate of diffusion of serum albumin (MW 6.9 x 10(4) D) out of beads of calcium alginate gels depends upon the concentration and uronic acid composition of the alginate (ManA/GulA ratio), the conditions under which the beads are produced, the pH, and the temperature. The diffusion coefficient decreases with increasi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390210
更新日期:1992-01-20 00:00:00
abstract::Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24853
更新日期:2013-07-01 00:00:00
abstract::The characteristics of horseradish peroxidase (HRP) immobilized onto Sepharose by a photochemical-initiated graft copolymerization are presented. Active copolymers were synthesized using different amounts of glycidylmethacrylate (GMA), bisacryloylpiperazine (BAP), or 1,3,5-hexhydrotriacryloyl-(s)-triazine (HTsT) as fu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250310
更新日期:1983-03-01 00:00:00
abstract::Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400609
更新日期:1992-09-01 00:00:00
abstract::Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25247
更新日期:2014-09-01 00:00:00
abstract::A phenomenological model of biocide action against microbial biofilms was derived. Processes incorporated in the model include bulk flow in and out of a well-mixed reactor, transport of dissolved species into the biofilm, substrate consumption by bacterial metabolism, bacterial growth, advection of cell mass within th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960220)49:4<445::AID-BIT
更新日期:1996-02-20 00:00:00
abstract::Several researchers have demonstrated that the presence of a heterologous protein in recombinant Escherichia coli elicits a response similar to the heat-shock response, which includes enhanced protease expression. The present work detects, quantifies, and characterizes intracellular protease activity in E. coli that a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420602
更新日期:1993-09-05 00:00:00
abstract::A computer-controlled headspace gas chromatograph was used to monitor the progress of ethanol production from both aerobic batch and anaerobic continuous fermentations. Using an automatic, electropneumatic sampling system, aliquots of fermentation headspace gas were injected directly onto the column for quantitative e...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251103
更新日期:1983-11-01 00:00:00
abstract::The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26725
更新日期:2018-09-01 00:00:00
abstract::A rapid method is developed to analyze the kinetics of thermal inactivation of enzymes that exhibit a nonlinear biphasic log(activity)-time relationship. Thermal destruction experiments on alcohol dehydrogenase from baker's yeast demonstrate the applicability of the method. The method is based on physical consideratio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960105)49:1<106::AID-BIT
更新日期:1996-01-05 00:00:00
abstract::The biotransformation of benzaldehyde and glucose to L-phenylacetylcarbinol (PAC) using Candida utilis was demonstrated in a solid-liquid two-phase partitioning bioreactor (TPPB) with the aim of reducing substrate, product, and by-product toxicity via sequestration. Previous work in the field had used octanol as the s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22839
更新日期:2010-11-01 00:00:00
abstract::We have used a high-throughput LC/MS/UV/ELSD method to rapidly determine the absolute quantity and purity of 42 organic compounds from seven lead discovery libraries. A general calibration curve generated from a different set of 42 compounds with seven different scaffolds was used in this analysis. We have also studie...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(2000)71:2<162::aid-bit1006>3.0.c
更新日期:2000-01-01 00:00:00
abstract::Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20959
更新日期:2006-08-20 00:00:00
abstract::Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20001220)70:6<619::aid-bit3>3.0.
更新日期:2000-12-20 00:00:00
abstract::Alginate gels produced by an external or internal gelation technique were studied so as to determine the optimal bead matrix within which DNA can be immobilized for in vivo application. Alginates were characterized for guluronic/mannuronic acid (G/M) content and average molecular weight using 1H-NMR and LALLS analysis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-02-20 00:00:00
abstract::For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21681
更新日期:2008-04-15 00:00:00
abstract::Cross-linked polyurethane (PU) was prepared for entrapping thermolysin. Using the immobilized thermolysin (IT), Z-L-aspartic acid (ZA) was reacted with -Lphenylalanine methyl ester (L-PM) in water-saturated ethyl acetate to give only alpha-Z-L-aspartylL-phenylalanine methyl ester (alpha-ZAPM). Ninety-four percent conv...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320504
更新日期:1988-08-20 00:00:00
abstract::Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlacto...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990105)62:1<30::aid-bit4
更新日期:1999-01-05 00:00:00
abstract::Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26797
更新日期:2018-10-01 00:00:00
abstract::The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27133
更新日期:2019-11-01 00:00:00
abstract::Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitab...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26534
更新日期:2018-05-01 00:00:00
abstract::A refolding strategy was described for on-column refolding of recombinant human interferon-gamma (rhIFN-gamma) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-gamma protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded bed ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20763
更新日期:2006-03-05 00:00:00
abstract::The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320204
更新日期:1988-07-05 00:00:00
abstract::A mechanistically detailed single-cell model E. coli B/r-A was adapted to simulate the effects of vector presence on cell metabolism. Competition for RNA polymerase between chromosome and plasmid DNA is explicitly included. Distribution of active ribosomes among chromosome- and plasmid-derived messenger RNA, another k...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290305
更新日期:1987-02-01 00:00:00
abstract::Vascular leak is a key driver of organ injury in diseases, and strategies that reduce enhanced permeability and vascular inflammation are promising therapeutic targets. Activation of the angiopoietin-1 (ANG1)-Tie2 tyrosine kinase signaling pathway is an important regulator of vascular quiescence. Here we describe the ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27580
更新日期:2021-01-01 00:00:00
abstract::Dielectric spectroscopy was applied to two industrial high cell density culture processes and used to determine on-line the concentration of CHO cells immobilized on macroporous microcarriers in a stirred bioreactor and in a packed-bed of disk carriers. The cell concentration predicted from the spectroscopic data was ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1197
更新日期:2002-02-05 00:00:00
abstract::This study addresses the characterization of human islet-like structures generated from a newly discovered sparse population of precursor cells (Petropavlovskaia and Rosenberg, 2002) in the human pancreas. These cells may be progenitor cells capable of producing pancreatic cells suitable for the treatment of type 1 di...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20801
更新日期:2006-04-05 00:00:00
abstract::By feeding ethanol at various high rates to low cell density cultures of Saccharomyces cerevisiae it was shown that the sharp fall in viability when ethanol is produced during rapid fermentations is in part a direct consequence of the high rate of change of extracellular ethanol concentration. Nevertheless, the fall i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260350202
更新日期:1990-01-20 00:00:00