Cellulase activity in commercial lipase preparations.

abstract：:A structured kinetic model describing growth, differentiation, and penicillin production in submerged Penicillium chrysogenum fermentations is reported. The filamentous hyphae are divided into four distinct regions on the basis of the activities and structure of hyphal compartments, viz., actively growing (mainly apic...

journal_title：Biotechnology and bioengineering

authors： Paul GC,Thomas CR

更新日期：1996-09-05 00:00:00

abstract：:The production of acetate by aerobically growing E. coli is examined. The problem is formulated in terms of a flow network that has as its objective maximal ATP synthesis. It is found that when loads are imposed and flux constraints exist either at the level of NADH turnover rate or the activity of a key Krebs cycle e...

journal_title：Biotechnology and bioengineering

authors： Majewski RA,Domach MM

更新日期：1990-03-25 00:00:00

abstract：:One unique to detect cytotoxicity is to utilize reporter gene assays for promoters that respond to stress-induced effects. In the present study, we discovered that the DNA sequence from nt -287 to +110 of the heat shock protein 70B' (HSP70B') gene could be used as a functional promoter to detect cytotoxicity of cadmiu...

journal_title：Biotechnology and bioengineering

authors： Wada K,Taniguchi A,Xu L,Okano T

更新日期：2005-11-20 00:00:00

abstract：:A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...

journal_title：Biotechnology and bioengineering

authors： Rozzi A,Castellazzi L,Speece RE

更新日期：2002-01-05 00:00:00

abstract：:We report the first direct images of the hydration of protein powders. Using an environmental scanning electron microscope (ESEM) we have taken a series of micrographs of a region of the enzyme (subtilisin) power whilst hydrating the sample. In addition, the sample has been viewed during exposure to toluene vapors. Th...

journal_title：Biotechnology and bioengineering

authors： Roziewski K,Russell AJ

更新日期：1992-05-01 00:00:00

abstract：:The coenzyme NAD plays a major role in metabolism as a key redox carrier and signaling molecule but current measurement techniques cannot distinguish between different compartment pools, between free and protein-bound forms and/or between NAD(H) and NADP(H). Local free NAD/NADH ratios can be determined from product/su...

journal_title：Biotechnology and bioengineering

authors： Canelas AB,van Gulik WM,Heijnen JJ

更新日期：2008-07-01 00:00:00

abstract：:In this study, white rot fungus, Polyporus brumalis, was applied to degrade dibutyl phthalate (DBP), a major environmental pollutant. The degradation potential and resulting products were evaluated with HPLC and GC/MS. As DBP concentration increased to 250, 750, and 1,250 microM, the mycelial growth of P. brumalis was...

journal_title：Biotechnology and bioengineering

authors： Lee SM,Lee JW,Koo BW,Kim MK,Choi DH,Choi IG

更新日期：2007-08-15 00:00:00

abstract：:Reduction and oxidation of steroids in the human gut are catalyzed by hydroxysteroid dehydrogenases of microorganisms. For the production of 12-ketochenodeoxycholic acid (12-Keto-CDCA) from cholic acid the biocatalytic application of the 12α-hydroxysteroid dehydrogenase of Clostridium group P, strain C 48-50 (HSDH) is...

journal_title：Biotechnology and bioengineering

authors： Braun M,Link H,Liu L,Schmid RD,Weuster-Botz D

更新日期：2011-06-01 00:00:00

abstract：:The increasing accessibility of mass isotopomer data via GC-MS and NMR technology has necessitated the use of a systematic and reliable method to take advantage of such data for flux analysis. Here we applied a nonlinear, optimization-based method to study substrate metabolism in cardiomyocytes using (13)C data from p...

journal_title：Biotechnology and bioengineering

authors： Vo TD,Palsson BO

更新日期：2006-12-05 00:00:00

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:Two general models for batch simultaneous enzymatic and microbial reaction (SEMR) processes are presented, the second derived from and simpler than the first and accounting for enzyme denaturation. Using the second model and parameter values from the literature, simulation was used to examine a range of enzyme additio...

journal_title：Biotechnology and bioengineering

authors： Asenjo JA,Sun WH,Spencer JL

更新日期：1991-05-01 00:00:00

abstract：:Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...

journal_title：Biotechnology and bioengineering

authors： Ritter A,Rauschert T,Oertli M,Piehlmaier D,Mantas P,Kuntzelmann G,Lageyre N,Brannetti B,Voedisch B,Geisse S,Jostock T,Laux H

更新日期：2016-11-01 00:00:00

abstract：:Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...

journal_title：Biotechnology and bioengineering

authors： Chen CI,Taylor RT

更新日期：1995-12-20 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:Recombinant protein glycosylation profiles have been shown to affect the in-vivo half-life, and therefore the efficacy and economics, for many therapeutics. While much research has been conducted correlating the effects of various stimuli on recombinant protein glycosylation characteristics, relatively little work has...

journal_title：Biotechnology and bioengineering

authors： Clark KJ,Griffiths J,Bailey KM,Harcum SW

更新日期：2005-06-05 00:00:00

abstract：:Modifications are made to an earlier thermodynamic model (TEEM1) for prediction of maximum microbial yields from aerobic and anaerobic as well as heterotrophic and autotrophic growth. The revised model (TEEM2) corrects for lower yields found with aerobic oxidations of organic compounds where an oxygenase is involved a...

journal_title：Biotechnology and bioengineering

authors： McCarty PL

更新日期：2007-06-01 00:00:00

abstract：:Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(e...

journal_title：Biotechnology and bioengineering

authors： Racine J,Cheradame H,Chu YS,Thiery JP,Rodriguez I

更新日期：2011-04-01 00:00:00

abstract：:Pseudomonas putida cells were grown in confined volumes in dual-membrane immobilized cell reactors constructed from microporous polyethylene hollow fibers and silicone rubber tubules as a model system for the study of mass transport in microbial aggregates. Local cell concentrations in the reactors reached 300 g dry m...

journal_title：Biotechnology and bioengineering

authors： Karel SF,Robertson CR

更新日期：1989-07-01 00:00:00

abstract：:A conceptual model describing the relative roles of sorption and precipitation processes for metals in solid-solution suspensions is presented. The model performance is demonstrated using experimental data on sorption and precipitation of metals in samples of activated sludge mixed liquor. Based on the experimental re...

journal_title：Biotechnology and bioengineering

authors： Kodukula PS,Patterson JW,Surampalli RY

更新日期：1994-04-15 00:00:00

abstract：:Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Marletta MA,Klibanov AM

更新日期：1987-07-01 00:00:00

abstract：:The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...

journal_title：Biotechnology and bioengineering

authors： Fava F,Bertin L

更新日期：1999-07-20 00:00:00

abstract：:The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...

journal_title：Biotechnology and bioengineering

authors： Gilbert PA,Kamen A,Bernier A,Garnier A

更新日期：2007-09-01 00:00:00

abstract：:This study addresses the characterization of human islet-like structures generated from a newly discovered sparse population of precursor cells (Petropavlovskaia and Rosenberg, 2002) in the human pancreas. These cells may be progenitor cells capable of producing pancreatic cells suitable for the treatment of type 1 di...

journal_title：Biotechnology and bioengineering

authors： Bodnar CA,Sen A,Kallos MS,Behie LA,Petropavlovskaia M,Rosenberg L

更新日期：2006-04-05 00:00:00

abstract：:Membrane adsorbers may be a viable alternative to the packed-bed chromatography for clearance of virus, host cell proteins, DNA, and other trace impurities. However, incorporation of membrane adsorbers into manufacturing processes has been slow due to the significant cost associated with obtaining regulatory approval ...

journal_title：Biotechnology and bioengineering

authors： Weaver J,Husson SM,Murphy L,Wickramasinghe SR

更新日期：2013-02-01 00:00:00

abstract：:Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...

journal_title：Biotechnology and bioengineering

authors： Shimizu K,Kobayashi T,Nagara A,Matsubara M

更新日期：1985-05-01 00:00:00

abstract：:Perfusion bioreactors have been used in different tissue engineering applications because of their consistent distribution of nutrients and flow-induced shear stress within the tissue-engineering scaffold. A widely used configuration uses a scaffold with a circular cross-section enclosed within a cylindrical chamber a...

journal_title：Biotechnology and bioengineering

authors： Hidalgo-Bastida LA,Thirunavukkarasu S,Griffiths S,Cartmell SH,Naire S

更新日期：2012-04-01 00:00:00

abstract：:A priori knowledge of the dissolved oxygen (O2) concentration profile within a hepatic hollow fiber (HF) bioreactor is important in developing an effective bioartificial liver assist device (BLAD). O2 provision is limiting within HF bioreactors and we hypothesize that supplementing a hepatic HF bioreactor's circulatin...

journal_title：Biotechnology and bioengineering

authors： Sullivan JP,Gordon JE,Palmer AF

更新日期：2006-02-05 00:00:00

abstract：:Hydrocarbon oils of the alga Botryococcus braunii, extracted from a natural "bloom" of the plant, have been hydrocracked to produce a distillate comprising 67% gasoline fraction, 15% aviation turbine fuel fraction, 15% diesel fuel fraction, and 3% residual oil. The distillate was examined by a number of standard petro...

journal_title：Biotechnology and bioengineering

authors： Hillen LW,Pollard G,Wake LV,White N

更新日期：1982-01-01 00:00:00

abstract：:The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...

journal_title：Biotechnology and bioengineering

authors： Prokop A,Ratcliffe HD,Fatayer MI,Al-Awadhi N,Khamis A,Murad M,Bond C,Hamdan IY

更新日期：1984-09-01 00:00:00

abstract：:The initial steps of glycerol dissimilation and 1,3-propanediol (1, 3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The...

journal_title：Biotechnology and bioengineering

authors： Ahrens K,Menzel K,Zeng A,Deckwer W

更新日期：1998-09-05 00:00:00