A structured model for hyphal differentiation and penicillin production using Penicillium chrysogenum.

abstract：:Aryl-alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2 O2 . Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has hitherto been hampered by the lack of appropriate heterologous express...

journal_title：Biotechnology and bioengineering

authors： Viña-Gonzalez J,Elbl K,Ponte X,Valero F,Alcalde M

更新日期：2018-07-01 00:00:00

abstract：:We present a mathematical model which includes TNF-alpha initiated survival and apoptotic cascades, as well as nuclear transcription of IkappaB. These pathways play a crucial role in deciding cell fate in response to inflammation and infection. Our model incorporates known specific protein-protein interactions as iden...

journal_title：Biotechnology and bioengineering

authors： Rangamani P,Sirovich L

更新日期：2007-08-01 00:00:00

abstract：:A novel deuterium ((2)H) NMR technique as developed for measuring the total number of deuterons exchanged by lyophilised protein samples following hydrogen-deuterium (H-D) exchange. Using this methodology differences in the H-D exchange behaviour of the proteolytic enzyme subtilisin Carlsberg hydrated either in air or...

journal_title：Biotechnology and bioengineering

authors： Hutcheon GA,Parker MC,Moore BD

更新日期：2000-11-05 00:00:00

abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

journal_title：Biotechnology and bioengineering

authors： Vavilin VA

更新日期：1983-06-01 00:00:00

abstract：:A model for radial flow mammalian cell culture has been developed. Two situations, one with permeable hollow fibers and the other with a porous matrix in the annulus, were considered. The hollow fibers were modeled as continuously distributed sinks. Numerical solutions are presented for the complete model as well as l...

journal_title：Biotechnology and bioengineering

authors： Tharakan JP,Chau PC

更新日期：1987-04-01 00:00:00

abstract：:The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

journal_title：Biotechnology and bioengineering

authors： Kheirolomoom A,Katoh S,Sada E,Yoshida K

更新日期：1991-04-15 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00

abstract：:The influence of fluid-dynamic conditions on the yield of Phaeodactylum tricornutum microalgal cultures was analyzed in two stages: first, the influence of air flow rate; second, the influence of using fluid-moving pumps for recirculating the culture. With respect to the air flow rate, the yield of the cultures increa...

journal_title：Biotechnology and bioengineering

authors： Alías CB,García-Malea López MC,Acién Fernández FG,Ferníndez Sevilla JM,García Sánchez JL,Molina Grima E

更新日期：2004-09-20 00:00:00

abstract：:The key to achieving successful, reproducible results following the introduction of beneficial microbes into soil relies on the survival rate of the inoculated bacteria in a heterogeneous soil environment and hence an improved encapsulation method was developed. Owing to the constraints associated with the inoculum fo...

journal_title：Biotechnology and bioengineering

authors： Young CC,Rekha PD,Lai WA,Arun AB

更新日期：2006-09-05 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main limiting steps for the hybridization to occur remain unclear. In here, FISH is approache...

journal_title：Biotechnology and bioengineering

authors： Lima JF,Maia P,T Magalhães B,Cerqueira L,Azevedo NF

更新日期：2020-10-01 00:00:00

abstract：:A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

journal_title：Biotechnology and bioengineering

authors： Niranjan SC,San KY

更新日期：1989-08-05 00:00:00

abstract：:Lyophilized proteins exhibit "pH memory," i.e., their behavior in the solid form corresponds to the pH of the aqueous solution from which they were freeze dried. Herein, we directly tested whether the ionization state is "remembered" by model organic compounds containing various protein functional groups (amino, carbo...

journal_title：Biotechnology and bioengineering

authors： Costantino HR,Griebenow K,Langer R,Klibanov AM

更新日期：1997-02-05 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial ce...

journal_title：Biotechnology and bioengineering

authors： Kang BC,Lee SY,Chang HN

更新日期：1993-11-05 00:00:00

abstract：:Escherichia coli has been the host organism most frequently investigated for efficient recombinant protein production. However, the production of a foreign protein in recombinant E. coli often leads to growth deterioration and elevated secretion of acetic acid. Such observed phenomena have been widely linked with cell...

journal_title：Biotechnology and bioengineering

authors： Zeng H,Yang A

更新日期：2019-06-01 00:00:00

abstract：:This article provides the analysis on biomass yields and energetic yields of the oleaginous yeasts. The biomass yields of the oleaginous yeasts are consistently lower than nonoleaginous microorganisms, whereas their energetic yields are higher. Data inconsistencies of literature are explained by the variation of energ...

journal_title：Biotechnology and bioengineering

authors： Pan JG,Rhee JS

更新日期：1986-01-01 00:00:00

abstract：:For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...

journal_title：Biotechnology and bioengineering

authors： Kwon YJ,Peng CA

更新日期：2005-05-05 00:00:00

abstract：:A microscale model for the transport and coupled reaction of microbes and chemicals in an idealized two-dimensional porous media has been developed. This model includes the flow, transport, and bioreaction of nutrients, electron acceptors, and microbial cells in a saturated granular porous media. The fluid and chemica...

journal_title：Biotechnology and bioengineering

authors： Dillon R,Fauci L

更新日期：2000-06-05 00:00:00

abstract：:Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature profiles were fairly similar up to 55 degrees C. However, above this temper...

journal_title：Biotechnology and bioengineering

authors： Dickensheets PA,Chen LF,Tsao GT

更新日期：1977-03-01 00:00:00

abstract：:The success of in situ bioremediation is often limited by the inability to bring bacteria in contact with the pollutant, which they will degrade. A bench-scale model aquifer was used to evaluate the impact of chemotaxis on the migration of bacteria toward the source of a chemical pollutant. The model was packed with s...

journal_title：Biotechnology and bioengineering

authors： Strobel KL,McGowan S,Bauer RD,Griebler C,Liu J,Ford RM

更新日期：2011-09-01 00:00:00

abstract：:Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

journal_title：Biotechnology and bioengineering

authors： Davidson AJ,Ellis MJ,Chaudhuri JB

更新日期：2010-08-15 00:00:00

abstract：:Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sen...

journal_title：Biotechnology and bioengineering

authors： Woolston BM,Roth T,Kohale I,Liu DR,Stephanopoulos G

更新日期：2018-01-01 00:00:00

abstract：:Innovation in monoclonal antibody (mAb) production continues to be driven by cell engineering strategies to increase yield and improve product quality. In a previous study, to investigate the effectiveness of transporter overexpression strategies, we prepared a taurine transporter-overexpressing host cell line (DXB11/...

journal_title：Biotechnology and bioengineering

authors： Tabuchi H,Sugiyama T

更新日期：2013-08-01 00:00:00

abstract：:In crude extract-based cell-free protein synthesis (CFPS), DNA templates are transcribed and translated into functional proteins. Although linear expression templates (LETs) are less laborious and expensive to generate, plasmid templates are often desired over polymerase chain reaction-generated LETs due to increased ...

journal_title：Biotechnology and bioengineering

authors： Zhu B,Gan R,Cabezas MD,Kojima T,Nicol R,Jewett MC,Nakano H

更新日期：2020-08-20 00:00:00

abstract：:Two kinds of disposable bioreactors, air-lift disposable bioreactors (ADB) and wave disposable bioreactors (WDB) were compared with stirred-tank reactors (5-L STR). These bioreactors were successfully applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immu...

journal_title：Biotechnology and bioengineering

authors： Kwon JY,Yang YS,Cheon SH,Nam HJ,Jin GH,Kim DI

更新日期：2013-09-01 00:00:00

abstract：:Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...

journal_title：Biotechnology and bioengineering

authors： Mehta KK,Paskaleva EE,Wu X,Grover N,Mundra RV,Chen K,Zhang Y,Yang Z,Feng H,Dordick JS,Kane RS

更新日期：2016-12-01 00:00:00

abstract：:Osteochondral tissue engineering aims to regenerate functional tissue-mimicking physiological properties of injured cartilage and its subchondral bone. Given the distinct structural and biochemical difference between bone and cartilage, bilayered scaffolds, and bioreactors are commonly employed. We present an osteocho...

journal_title：Biotechnology and bioengineering

authors： Xue R,Chung B,Tamaddon M,Carr J,Liu C,Cartmell SH

更新日期：2019-11-01 00:00:00

abstract：:The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-a...

journal_title：Biotechnology and bioengineering

authors： Dean JP,Dervakos GA

更新日期：1998-04-05 00:00:00

abstract：:Recombinant retroviruses are effective vectors able to integrate transgenes into the target cell's genome to achieve longer-term expression. This study investigates the effect of cell lysis products, a common cell culture by-product, on the transduction of suspension cells by gammaretroviral vectors. Cell lysates deri...

journal_title：Biotechnology and bioengineering

authors： Beauchesne PR,Bruce KJ,Bowen BD,Piret JM

更新日期：2010-04-15 00:00:00

abstract：:trans-4-Hydroxy- l-proline (Hyp) is an abundant component of mammalian collagen and functions as a chiral synthon for the syntheses of anti-inflammatory drugs in the pharmaceutical industry. Proline 4-hydroxylase (P4H) can catalyze the conversion of l-proline to Hyp; however, it is still challenging for the fermentati...

journal_title：Biotechnology and bioengineering

authors： Zhang Y,Zhang Y,Shang X,Wang B,Hu Q,Liu S,Wen T

更新日期：2019-01-01 00:00:00