Reaction characteristics and stability of a membrane-bound enzyme reconstituted in bilayers of liposomes.

abstract：:In the process of producing ethanol from lignocellulosic materials such as wheat straw, compounds that can act inhibitory to enzymatic hydrolysis and to cellular growth may be generated during the pretreatment. Ethanol production was evaluated on pretreated wheat straw hydrolysate using four different recombinant Sacc...

journal_title：Biotechnology and bioengineering

authors： Panagiotou G,Olsson L

更新日期：2007-02-01 00:00:00

abstract：:Extracellular beta-xylosidase (1,4-beta-D-xylan xylohydrolase, EC 3.2.1.37) from culture filtrates of Neurospora crassa was purified to homogeneity by preparative isoelectric focusing followed by gel electrophoresis. The molecular weight of the purified xylosidase was 83,000 D and the K(m) on p-nitrophenyl-beta-D-xylo...

journal_title：Biotechnology and bioengineering

authors： Deshpande V,Lachke A,Mishra C,Keskar S,Rao M

更新日期：1986-12-01 00:00:00

abstract：:Multivalent cations have been known to be important components of activated sludge floc structure due to their bridging ability of the negatively charged sites on the biopolymer network. Recently in batch systems it was found that excess concentration of monovalent cations led to the deterioration in settleability, de...

journal_title：Biotechnology and bioengineering

authors： Kara F,Gurakan GC,Sanin FD

更新日期：2008-06-01 00:00:00

abstract：:Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...

journal_title：Biotechnology and bioengineering

authors： Cheng SY,Constantinidis I,Sambanis A

更新日期：2006-04-20 00:00:00

abstract：:Disulfide-rich peptides isolated from cone snails are of great interest as drug leads due to their high specificity and potency toward therapeutically relevant ion channels and receptors. They commonly contain the inhibitor cystine knot (ICK) motif comprising three disulfide bonds forming a knotted core. Here we repor...

journal_title：Biotechnology and bioengineering

authors： Kwon S,Bosmans F,Kaas Q,Cheneval O,Conibear AC,Rosengren KJ,Wang CK,Schroeder CI,Craik DJ

更新日期：2016-10-01 00:00:00

abstract：:Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed)...

journal_title：Biotechnology and bioengineering

authors： Wu LQ,Chen T,Wallace KK,Vazquez-Duhalt R,Payne GF

更新日期：2001-12-01 00:00:00

abstract：:A production process for B. thuringiensis (Bt) bioinsecticides was designed in detail, including alternative batch, low-density fed-batch (LDFB), and high-density fed-batch (HDFB) fermentation configurations. Capital and operating costs, as well as profitability based on simple rate of return, were performed using a p...

journal_title：Biotechnology and bioengineering

authors： Rowe GE,Margaritis A

更新日期：2004-05-20 00:00:00

abstract：:We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. Pseudomonas fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfull...

journal_title：Biotechnology and bioengineering

authors： Lee SH,Choi JI,Han MJ,Choi JH,Lee SY

更新日期：2005-04-20 00:00:00

abstract：:Trichoplusia ni larvae were infected with baculoviruses containing genes for the expression of ultraviolet optimized green fluorescent protein (GFPuv) and several product proteins. A GFP-specific optical probe was used to both excite the green fluorescent protein (lambda(ex) = 385 nm), and subsequently monitor fluores...

journal_title：Biotechnology and bioengineering

authors： Kramer SF,Kostov Y,Rao G,Bentley WE

更新日期：2003-07-20 00:00:00

abstract：:Biofouling is the undesirable adhesion and development of microorganisms and macroorganisms in a water environment. An electrochemical antifouling system based on management of primary adhesion of microorganisms was developed employing titanium electrode for antifouling of seawater cooling pipes and marine infrastruct...

journal_title：Biotechnology and bioengineering

authors： Wake H,Takahashi H,Takimoto T,Takayanagi H,Ozawa K,Kadoi H,Okochi M,Matsunaga T

更新日期：2006-10-20 00:00:00

abstract：:A number of bioreactor configurations have been developed for the manufacture of products from mammalian cell hosts. Even in the most efficient of these, however, problems such as nutrient exhaustion, growth factor deprivation, and toxin accumulations may arise. Consequently, the current effort focused on the feasibil...

journal_title：Biotechnology and bioengineering

authors： Mastrangelo AJ,Hardwick JM,Zou S,Betenbaugh MJ

更新日期：2000-03-05 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:A pure culture of Pseudomonas fluorescens was used as a model system to study the kinetics of denitrification. An exponentially growing culture was harvested and resuspended in an anoxic acetate solution buffered with K/Na phosphate at pH values of 6.6, 7.0, 7.4, and 7.8. The temperature was kept at 28 degrees C in al...

journal_title：Biotechnology and bioengineering

authors： Almeida JS,Reis MA,Carrondo MJ

更新日期：1995-06-05 00:00:00

abstract：:Working conditions of a biochemical fuel cell formed by an oxygen cathode and a platinum bioanode in a Saccharomyces cerevisiae suspension metabolizing glucose are described. The biocell response in terms of bioanode potential and current drainage under different fermentation conditions is reported. A kinetic equation...

journal_title：Biotechnology and bioengineering

authors： Videla HA,Arvía AJ

更新日期：1975-10-01 00:00:00

abstract：:The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...

journal_title：Biotechnology and bioengineering

authors： Fox SR,Patel UA,Yap MG,Wang DI

更新日期：2004-01-20 00:00:00

abstract：:Recombinant cell growth and protein synthesis by a recombinant Escherichia coli under various inducing conditions are compared to the predictions of a mathematical model. The mathematical model used was a combination of two literature models: (1) an empirical kinetic model for recombinant growth and product formation ...

journal_title：Biotechnology and bioengineering

authors： Betenbaugh MJ,Dhurjati P

更新日期：1990-06-20 00:00:00

abstract：:Clostridium carboxidivorans P7 is one of three microbial catalysts capable of fermenting synthesis gas (mainly CO, CO(2) , and H(2) ) to produce the liquid biofuels ethanol and butanol. Gasification of feedstocks to produce synthesis gas (syngas), followed by microbial conversion to solvents, greatly expands the diver...

journal_title：Biotechnology and bioengineering

authors： Ukpong MN,Atiyeh HK,De Lorme MJ,Liu K,Zhu X,Tanner RS,Wilkins MR,Stevenson BS

更新日期：2012-11-01 00:00:00

abstract：:Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a s...

journal_title：Biotechnology and bioengineering

authors： Sucosky P,Osorio DF,Brown JB,Neitzel GP

更新日期：2004-01-05 00:00:00

abstract：:The enantiocatalytic performance of immobilized lipase in an emulsion membrane reactor using stable emulsion prepared by membrane emulsification technology was studied. The production of optical pure (S)-naproxen from racemic naproxen methyl ester was used as a model reaction system. The O/W emulsion, containing the s...

journal_title：Biotechnology and bioengineering

authors： Giorno L,Li N,Drioli E

更新日期：2003-12-20 00:00:00

abstract：:Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and t...

journal_title：Biotechnology and bioengineering

authors： Bok M,Li H,Yeo LY,Friend JR

更新日期：2009-06-01 00:00:00

abstract：:After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...

journal_title：Biotechnology and bioengineering

authors： Marek M,Valentová O,Kás J

更新日期：1984-10-01 00:00:00

abstract：:Biomanufacturing exhibits inherent variability that can lead to variation in performance attributes and batch failure. To help ensure process consistency and product quality the development of predictive models and integrated control strategies is a promising approach. In this study, a feedback controller was develope...

journal_title：Biotechnology and bioengineering

authors： Schmitt J,Downey B,Beller J,Russell B,Quach A,Lyon D,Curran M,Mulukutla BC,Chu C

更新日期：2019-09-01 00:00:00

abstract：:Light-responsive bioconjugates are synthesized by a two-step protocol calling first for cotelomerization (chain-transfer polymerization) of N-isopropylacrylamide and N-acryloxysuccinimide. The desired bioligand (biotin) is used in modified form as chain-transfer agent in this step. As a consequence, 100% of the produc...

journal_title：Biotechnology and bioengineering

authors： Desponds A,Freitag R

更新日期：2005-09-05 00:00:00

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00

abstract：:The cellulase production from immobilized Trichoderma reesei composites prepared by radiation polymerization at low temperature was studied. The production of cellulase from the cells irradiated by radiation was slightly retarded at the initial stage of the culture, but was immediately recovered. The production of cel...

journal_title：Biotechnology and bioengineering

authors： Kumakura M,Kaetsu I,Nisizawa K

更新日期：1984-01-01 00:00:00

abstract：:The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...

journal_title：Biotechnology and bioengineering

authors： Kunert R,Steinfellner W,Purtscher M,Assadian A,Katinger H

更新日期：2000-01-05 00:00:00

abstract：:Spores, although often considered metabolically inert, catalyze a variety of reactions. The use of spores instead of mycelium for bioconversions has several advantages. In this paper, we describe the difference in susceptibility of mycelium and spores against toxic substrates and products. A higher resistance of spore...

journal_title：Biotechnology and bioengineering

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2002-12-20 00:00:00

abstract：:Rare sugars have various known biological functions and potential for applications in pharmaceutical, cosmetics, and food industries. Here we designed and constructed a recombination pathway in Corynebacterium glutamicum, in which dihydroxyacetone phosphate (DHAP), an intermediate of the glycolytic pathway, and a vari...

journal_title：Biotechnology and bioengineering

authors： Yang J,Zhu Y,Li J,Men Y,Sun Y,Ma Y

更新日期：2015-01-01 00:00:00

abstract：:In crude extract-based cell-free protein synthesis (CFPS), DNA templates are transcribed and translated into functional proteins. Although linear expression templates (LETs) are less laborious and expensive to generate, plasmid templates are often desired over polymerase chain reaction-generated LETs due to increased ...

journal_title：Biotechnology and bioengineering

authors： Zhu B,Gan R,Cabezas MD,Kojima T,Nicol R,Jewett MC,Nakano H

更新日期：2020-08-20 00:00:00

abstract：:Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation. In the latter case, trapping efficiencies of up to 100% could be obtained. Activities ...

journal_title：Biotechnology and bioengineering

authors： Mosmuller EW,Franssen MC,Engbersen JF

更新日期：1993-06-20 00:00:00