Abstract:
:Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a spinner flask operating under conditions used to produce cartilage. Laboratory experiments carried out in a scaled-up model bioreactor employ particle-image velocimetry (PIV) to determine velocity and shear-rate fields in the vicinity of the construct closest to the stir bar, in addition to turbulence properties. Numerical computations calculated using FLUENT, a commercial software package, simulate the flow field in the same model bioreactor under similar operating conditions. In the computations, scaffolds were modeled as both solid and porous media with different permeabilities and flow rates through various faces of the construct nearest the stir bar were examined.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Sucosky P,Osorio DF,Brown JB,Neitzel GPdoi
10.1002/bit.10788subject
Has Abstractpub_date
2004-01-05 00:00:00pages
34-46issue
1eissn
0006-3592issn
1097-0290journal_volume
85pub_type
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