Biodegradation of 3,4-dichloroaniline in a fluidized bed bioreactor and a steady-state biofilm Kinetic model.

abstract：:Many microorganisms are capable of sequestering and concentrating heavy metals from their aqueous environment. While much research has beep carried out on the uptake of single species of metal ions, little attention seems to have been given to the study of multimetal ion systems. A mathematical model has previously be...

journal_title：Biotechnology and bioengineering

authors： Ting YP,Prince IG,Lawson F

更新日期：1991-03-05 00:00:00

abstract：:In order to evaluate the sensitivity of hepatocellular cultures to variations in both substrate stiffness and bioactive ligand presentation, hepatocytes were cultured on differentially compliant polyacrylamide gel discs functionalized with varying amounts of the ECM ligand, fibronectin (FN). Subconfluent cell cultures...

journal_title：Biotechnology and bioengineering

authors： Semler EJ,Lancin PA,Dasgupta A,Moghe PV

更新日期：2005-02-05 00:00:00

abstract：:We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

journal_title：Biotechnology and bioengineering

authors： Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

更新日期：2017-08-01 00:00:00

abstract：:A rapid method is developed to analyze the kinetics of thermal inactivation of enzymes that exhibit a nonlinear biphasic log(activity)-time relationship. Thermal destruction experiments on alcohol dehydrogenase from baker's yeast demonstrate the applicability of the method. The method is based on physical consideratio...

journal_title：Biotechnology and bioengineering

authors： Nath S

更新日期：1996-01-05 00:00:00

abstract：:Helical-blade solids mixers have a large potential as bioreactors for solid-state fermentation (SSF). Fundamental knowledge of the flow and mixing behavior is required for robust operation of these mixers. In this study predictions of a discrete particle model were compared to experiments with colored wheat grain part...

journal_title：Biotechnology and bioengineering

authors： Schutyser MA,Briels WJ,Rinzema A,Boom RM

更新日期：2003-10-05 00:00:00

abstract：:RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger RNA (mRNA) splicing, has thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclona...

journal_title：Biotechnology and bioengineering

authors： Tzani I,Monger C,Motheramgari K,Gallagher C,Hagan R,Kelly P,Costello A,Meiller J,Floris P,Zhang L,Clynes M,Bones J,Barron N,Clarke C

更新日期：2020-08-01 00:00:00

abstract：:NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 ...

journal_title：Biotechnology and bioengineering

authors： Seth G,Philp RJ,Denoya CD,McGrath K,Stutzman-Engwall KJ,Yap M,Hu WS

更新日期：2005-06-05 00:00:00

abstract：:Two general models for batch simultaneous enzymatic and microbial reaction (SEMR) processes are presented, the second derived from and simpler than the first and accounting for enzyme denaturation. Using the second model and parameter values from the literature, simulation was used to examine a range of enzyme additio...

journal_title：Biotechnology and bioengineering

authors： Asenjo JA,Sun WH,Spencer JL

更新日期：1991-05-01 00:00:00

abstract：:A combined approach based on the use of ATR-FT/IR and steady-state fluorescence spectroscopy allowed to shed light on the effects of the additive methoxypolyethylene glycol (MePEG) on the hydration, conformation and dynamic properties of lipase from Burkholderia cepacia dehydrated to form a film. Spectroscopic data sh...

journal_title：Biotechnology and bioengineering

authors： Secundo F,Barletta G,Mazzola G

更新日期：2008-10-01 00:00:00

abstract：:We have developed a simple and robust transient expression system utilizing the 25 kDa branched cationic polymer polyethylenimine (PEI) as a vehicle to deliver plasmid DNA into suspension-adapted Chinese hamster ovary cells synchronized in G2/M phase of the cell cycle by anti-mitotic microtubule disrupting agents. The...

journal_title：Biotechnology and bioengineering

authors： Tait AS,Brown CJ,Galbraith DJ,Hines MJ,Hoare M,Birch JR,James DC

更新日期：2004-12-20 00:00:00

abstract：:A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isoc...

journal_title：Biotechnology and bioengineering

authors： Katti A,Huang JX,Guiochon G

更新日期：1990-07-01 00:00:00

abstract：:Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...

journal_title：Biotechnology and bioengineering

authors： Gupta KG,Yadav NK,Dhawan S

更新日期：1978-12-01 00:00:00

abstract：:The major objectives for preparative protein chromatography are maximal loading and increased flow rate while maintaining defined resolution. Conventionally a series of chromatographic experiments are performed and the optimal conditions are selected according to the separation criteria. Computer-aided process design ...

journal_title：Biotechnology and bioengineering

authors： Jungbauer A,Kaltenbrunner O

更新日期：1996-10-20 00:00:00

abstract：:Generating stable, high-producing cell lines for recombinant protein production requires an understanding of the potential limitations in the cellular machinery for protein expression. In order to increase our understanding of what makes a stable high producer, we have generated a panel of 17 recombinant monoclonal an...

journal_title：Biotechnology and bioengineering

authors： Chusainow J,Yang YS,Yeo JH,Toh PC,Asvadi P,Wong NS,Yap MG

更新日期：2009-03-01 00:00:00

abstract：:Reactor systems based on tapered fluidized beds are being developed for aqueous bioprocesses in which adhering microorganisms or immobilized active biological fractions are used. The use of a fluidized bed prevents biomass buildup, accommodates particulates in the feed stream, is compatible with gas sparging, and allo...

journal_title：Biotechnology and bioengineering

authors： Scott CD,Hancher CW

更新日期：1976-10-01 00:00:00

abstract：:Catalytic activity of lipases (from Rhizopus arrhizus, Canadida rugosa, and Pseudomonas sp. was studied in organic media, mainly diisopropyl ether. The effect of water activity (a(w)) on V(max) showed that the enzyme activity in general increased with increasing amounts of water for the three enzymes. This was shown b...

journal_title：Biotechnology and bioengineering

authors： Wehtje E,Adlercreutz P

更新日期：1997-09-05 00:00:00

abstract：:The inhibitory effect of ethanol on yeast growth and fermentation has been studied for the strain Saccharomyces cerevisiae ATCC No. 4126 under anaerobic batch conditions. The results obtained reveal that there is no striking difference between the response of growth and ethanol fermentation. Two kinetic models are als...

journal_title：Biotechnology and bioengineering

authors： Luong JH

更新日期：1985-03-01 00:00:00

abstract：:Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...

journal_title：Biotechnology and bioengineering

authors： Criddle CS

更新日期：1993-05-01 00:00:00

abstract：:Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

journal_title：Biotechnology and bioengineering

authors： Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

更新日期：2020-01-01 00:00:00

abstract：:In order to predict the potential benefit associated with mixing devices designed to introduce periodic light modulations in dense cultures of microalgae, it is necessary to develop a quantitative understanding of the relationship between the frequency of the modulations and the resulting photosynthetic efficiency enh...

journal_title：Biotechnology and bioengineering

authors： Terry KL

更新日期：1986-07-01 00:00:00

abstract：:The production of volatile industrial chemicals utilizing metabolically engineered extreme thermophiles offers the potential for processes with simultaneous fermentation and product separation. An excellent target chemical for such a process is acetone (Tb  = 56°C), ideally produced from lignocellulosic biomass. Caldi...

journal_title：Biotechnology and bioengineering

authors： Straub CT,Bing RG,Otten JK,Keller LM,Zeldes BM,Adams MWW,Kelly RM

更新日期：2020-08-08 00:00:00

abstract：:A simple feeding strategy was developed and successfully employed for nutritional control in a 2-L fed-batch culture of hybridoma cells. A previously developed stoichiometric model for animal cell growth was used to design a supplemental medium for feeding. Undialyzed fetal bovine serum and trace metals (Fe(2+), SeO(3...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1996-09-20 00:00:00

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:The redox control bioreactor (RCB) is a new hollow fiber membrane bioreactor (HFMBR) design in which oxygen and hydrogen gases are provided simultaneously through separate arrays of juxtaposed hollow fiber (HF) membranes. This study applied the RCB for completely autotrophic conversion of ammonia to N(2) through nitri...

journal_title：Biotechnology and bioengineering

authors： Smith DP,Rector T,Reid-Black K,Hummerick M,Strayer R,Birmele M,Roberts MS,Garland JL

更新日期：2008-03-01 00:00:00

abstract：:Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

journal_title：Biotechnology and bioengineering

authors： Vemuri GN,Eiteman MA,Altman E

更新日期：2006-06-20 00:00:00

abstract：:Ethanol-producing bioreactors employing cells of Zymomonas mobilis attached to glass-fiber pads were operated continuously for as long as 28 days. Ethanol production, which is related to bed-associated biomass levels, was found to occur in three distinct phases: an exponential phase, a linear phase, and a "steady-stat...

journal_title：Biotechnology and bioengineering

authors： Arcuri EJ

更新日期：1982-03-01 00:00:00

abstract：:One unique to detect cytotoxicity is to utilize reporter gene assays for promoters that respond to stress-induced effects. In the present study, we discovered that the DNA sequence from nt -287 to +110 of the heat shock protein 70B' (HSP70B') gene could be used as a functional promoter to detect cytotoxicity of cadmiu...

journal_title：Biotechnology and bioengineering

authors： Wada K,Taniguchi A,Xu L,Okano T

更新日期：2005-11-20 00:00:00

abstract：:Pre-filtration using ion exchange membrane adsorbers can improve parvovirus filter throughput of monoclonal antibodies (mAbs). The membranes work by binding trace foulants, and although some antibody product also binds, yields > or =99% are easily achieved by overloading. Results show that foulant adsorption is depend...

journal_title：Biotechnology and bioengineering

authors： Brown A,Bechtel C,Bill J,Liu H,Liu J,McDonald D,Pai S,Radhamohan A,Renslow R,Thayer B,Yohe S,Dowd C

更新日期：2010-07-01 00:00:00

abstract：:The engineering of production cell lines to express anti-apoptotic genes has been pursued in recent years due to potential process benefits, including enhanced cell survival, increased protein expression, and improved product quality. In this study, a baby hamster kidney cell line secreting recombinant factor VIII (BH...

journal_title：Biotechnology and bioengineering

authors： Nivitchanyong T,Martinez A,Ishaque A,Murphy JE,Konstantinov K,Betenbaugh MJ,Thrift J

更新日期：2007-11-01 00:00:00

abstract：:Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...

journal_title：Biotechnology and bioengineering

authors： Livernoche D,Jurasek L,Desrochers M,Dorica J,Veliky IA

更新日期：1983-08-01 00:00:00