Abstract:
:A rapid method is developed to analyze the kinetics of thermal inactivation of enzymes that exhibit a nonlinear biphasic log(activity)-time relationship. Thermal destruction experiments on alcohol dehydrogenase from baker's yeast demonstrate the applicability of the method. The method is based on physical considerations (as opposed to mathematical curve fitting/regression methods) and also serves as a quick check of results obtained using nonlinear regression. It is superior to fitting nonlinear enzyme inactivation data by first-order kinetics or taking the initial and final slopes of the inactivation data. In fact, the method is of general validity and can be applied to any decay process that can be represented by a sum of exponentials.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Nath Sdoi
10.1002/(SICI)1097-0290(19960105)49:1<106::AID-BITsubject
Has Abstractpub_date
1996-01-05 00:00:00pages
106-10issue
1eissn
0006-3592issn
1097-0290journal_volume
49pub_type
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