New soluble angiopoietin analog of Hepta-ANG1 prevents pathological vascular leakage.

abstract：:Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

journal_title：Biotechnology and bioengineering

authors： Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

更新日期：2008-07-01 00:00:00

abstract：:Non-invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online d...

journal_title：Biotechnology and bioengineering

authors： Regestein L,Maskow T,Tack A,Knabben I,Wunderlich M,Lerchner J,Büchs J

更新日期：2013-05-01 00:00:00

abstract：:Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Marletta MA,Klibanov AM

更新日期：1987-07-01 00:00:00

abstract：:Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

journal_title：Biotechnology and bioengineering

authors： Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

更新日期：2008-01-01 00:00:00

abstract：:A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifyi...

journal_title：Biotechnology and bioengineering

authors： Terada A,Yamamoto T,Tsuneda S,Hirata A

更新日期：2006-07-05 00:00:00

abstract：:A general theory is presented in this article for determining the intrinsic rate constants for the main reaction and deactivation reaction, the effective diffusivity of the substrate, and the active enzyme distribution within porous solid supports from deactivation study of a continuous stirred-basket reactor (CSBR). ...

journal_title：Biotechnology and bioengineering

authors： Hossain MM,Do DD

更新日期：1989-03-01 00:00:00

abstract：:Efficient xylose utilization will facilitate microbial conversion of lignocellulosic sugar mixtures into valuable products. In Escherichia coli, xylose catabolism is controlled by carbon catabolite repression (CCR). However, in E. coli such as the succinate-producing strain KJ122 with disrupted CCR, xylose utilization...

journal_title：Biotechnology and bioengineering

authors： Kurgan G,Sievert C,Flores A,Schneider A,Billings T,Panyon L,Morris C,Taylor E,Kurgan L,Cartwright R,Wang X

更新日期：2019-08-01 00:00:00

abstract：:The patterning of cardiac myocytes on a micron scale ( approximately 5 microm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca(2+) transition o...

journal_title：Biotechnology and bioengineering

authors： Kaji H,Takoh K,Nishizawa M,Matsue T

更新日期：2003-03-20 00:00:00

abstract：:The sensitivity of a conceptual model of a foam emulsion bioreactor (FEBR) used for the control of toluene vapors in air was examined. Model parametric sensitivity studies showed which parameters affect the removal of toluene (as model pollutant) in the FEBR the most significantly, and enabled definition of the limits...

journal_title：Biotechnology and bioengineering

authors： Kan E,Deshusses MA

更新日期：2009-02-15 00:00:00

abstract：:The Model presented in this work demonstrates the combination of cell-cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady-state continuous culture. The cell-cycle model is based upon a population balance model as described by Cazzador et al. and assumes the existence o...

journal_title：Biotechnology and bioengineering

authors： Martens DE,Sipkema EM,de Gooijer CD,Beuvery EC,Tramper J

更新日期：1995-10-05 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:A 3D printed ultrafiltration/diafiltration (UF/DF) module is presented allowing the continuous, simultaneous concentration of retained (bio-)molecules and reduction or exchange of the salt buffer. Differing from the single-pass UF concepts known from the literature, DF operation does not require the application of sev...

journal_title：Biotechnology and bioengineering

authors： Tan R,Franzreb M

更新日期：2020-03-01 00:00:00

abstract：:Embryonic stem cells (ESCs) are an important source of cardiomyocytes for regenerating injured myocardium. The successful use of ESC-derived cardiomyocytes in cardiac tissue engineering requires an understanding of the important scaffold properties and culture conditions to promote cell attachment, differentiation, or...

journal_title：Biotechnology and bioengineering

authors： Parrag IC,Zandstra PW,Woodhouse KA

更新日期：2012-03-01 00:00:00

abstract：:The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and ...

journal_title：Biotechnology and bioengineering

authors： Zhang Z,Wang B,Hu Q,Sommerfeld M,Li Y,Han D

更新日期：2016-10-01 00:00:00

abstract：:A simple approach was developed to determine the half-saturation coefficient for dissolved oxygen (K(DO)) for three bacteria by maintaining a constant oxygen concentration in continuous culture, and employing a dynamic method to obtain the specific growth rate (mu) for each species. Measurement of mu at selected disso...

journal_title：Biotechnology and bioengineering

authors： Hao OJ,Richard MG,Jenkins D,Blanch HW

更新日期：1983-02-01 00:00:00

abstract：:Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the sin...

journal_title：Biotechnology and bioengineering

authors： Seo JH,Bailey JE

更新日期：1985-02-01 00:00:00

abstract：:A spinning cylindrical filter, known as a spinfilter, permits the mammalian cell bioreactor operation at high perfusion rates leading to very high cell densities (10(7) mL(-1)). Filter screens with openings (25 mum) slightly larger than the average cell size have been used to retain single cells in suspension over a l...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1994-01-20 00:00:00

abstract：:Alkyl beta-D-glucosides were synthesized from D-glucose and alcohols by reverse hydrolysis using the commercially available almond beta-D-glucosidase in 9:1 (v/v) acetonitrile-water medium. The main characteristics of this enzyme-catalyzed glucosylation were established by using 2-hydroxybenzyl alcohol. The reaction i...

journal_title：Biotechnology and bioengineering

authors： Vic G,Biton J,Le Beller D,Michel JM,Thomas D

更新日期：1995-04-20 00:00:00

abstract：:Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Partic...

journal_title：Biotechnology and bioengineering

authors： Kaup BA,Ehrich K,Pescheck M,Schrader J

更新日期：2008-02-15 00:00:00

abstract：:A means of rapidly renaturing denatured protein was devised and evaluated. Three liquids were laminarly layered in a centrifuge tube, in which two solutions sandwiched liquid paraffin so as to form a pseudolipid bilayer. Denatured and aggregted protein placed on the upper surface of liquid paraffin was renatured as it...

journal_title：Biotechnology and bioengineering

authors： Yoshii H,Kojima T,Furuta T,Yasunishi A

更新日期：1994-01-05 00:00:00

abstract：:The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...

journal_title：Biotechnology and bioengineering

authors： Junker B

更新日期：2001-07-05 00:00:00

abstract：:Protein switches have a variety of potential applications in biotechnology and medicine that motivate efforts to accelerate their development. Switches can be built by the proper fusion of two proteins with the prerequisite input and output functions. However, the exact fusion geometry for switch creation, which typic...

journal_title：Biotechnology and bioengineering

authors： Tullman J,Nicholes N,Dumont MR,Ribeiro LF,Ostermeier M

更新日期：2016-04-01 00:00:00

abstract：:Enzyme engineering is a powerful tool to fine-tune the enzymes. It is a technique by which the stability, activity, and specificity of the enzymes can be altered. The characteristic properties of an enzyme can be amended by immobilization and protein engineering. Among them, protein engineering is the most promising, ...

journal_title：Biotechnology and bioengineering

authors： Ali M,Ishqi HM,Husain Q

更新日期：2020-06-01 00:00:00

abstract：:The release kinetics of beta-galactosidase protein have been determined during small-scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramatically affect the...

journal_title：Biotechnology and bioengineering

authors： Feliu JX,Cubarsi R,Villaverde A

更新日期：1998-06-05 00:00:00

abstract：:Recombinant cell growth and protein synthesis by a recombinant Escherichia coli under various inducing conditions are compared to the predictions of a mathematical model. The mathematical model used was a combination of two literature models: (1) an empirical kinetic model for recombinant growth and product formation ...

journal_title：Biotechnology and bioengineering

authors： Betenbaugh MJ,Dhurjati P

更新日期：1990-06-20 00:00:00

abstract：:It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...

journal_title：Biotechnology and bioengineering

authors： Ngian KF,Lin SH,Martin WR

更新日期：1977-12-01 00:00:00

abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

journal_title：Biotechnology and bioengineering

authors： Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

更新日期：2008-08-01 00:00:00

abstract：:Chemostat cultivation of Aspergillus niger and other filamentous fungi is often hindered by the spontaneous appearance of morphologic mutants. Using the Variomixing bioreactor and applying different chemostat conditions we tried to optimize morphologic stability in both ammonium- and glucose-limited cultures. In most ...

journal_title：Biotechnology and bioengineering

authors： van de Vondervoort PJ,Poulsen BR,Ruijter GJ,Schuleit T,Visser J,Iversen JJ

更新日期：2004-05-05 00:00:00

abstract：:There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...

journal_title：Biotechnology and bioengineering

authors： Johnson SA,Brown MR,Lute SC,Brorson KA

更新日期：2017-01-01 00:00:00

abstract：:The use of cell numbers rather than mass to quantify the size of the biotic phase in animal cell cultures causes several problems. First, the cell size varies with growth conditions, thus yields expressed in terms of cell numbers cannot be used in the normal mass balance sense. Second, experience from microbial system...

journal_title：Biotechnology and bioengineering

authors： Nielsen LK,Reid S,Greenfield PF

更新日期：1997-11-20 00:00:00