Enzyme engineering: Reshaping the biocatalytic functions.

abstract：:Sugarcane bagasse is an abundant and sustainable resource, generated as a by-product of sugarcane milling. The cellulosic material within bagasse can be broken down into glucose molecules and fermented to produce ethanol, making it a promising feedstock for biofuel production. Mild acid pretreatment hydrolyses the hem...

journal_title：Biotechnology and bioengineering

authors： Greenwood AA,Farrell TW,O'Hara IM

更新日期：2013-12-01 00:00:00

abstract：:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

journal_title：Biotechnology and bioengineering

authors： Birnbaum S,Bailey JE

更新日期：1991-04-05 00:00:00

abstract：:In view of the biochemical reaction catalyzed by enzyme powder suspended in a water-insoluble organic solvent, an equation was derived to estimate the amount of water bound to the enzyme powder. With this equation, an apparent adsorption isotherm between free water (water freely dissolved in benzene) and bound water (...

journal_title：Biotechnology and bioengineering

authors： Yamane T,Kojima Y,Ichiryu T,Nagata M,Shimizu S

更新日期：1989-09-01 00:00:00

abstract：:Uranium contamination is a serious concern at several sites motivating the development of novel treatment strategies such as the Geobacter-mediated reductive immobilization of uranium. However, this bioremediation strategy has not yet been optimized for the sustained uranium removal. While several reactive-transport m...

journal_title：Biotechnology and bioengineering

authors： Zhao J,Scheibe TD,Mahadevan R

更新日期：2011-07-01 00:00:00

abstract：:In macroscopic dynamic models of fermentation processes, elementary modes (EM) derived from metabolic networks are often used to describe the reaction stoichiometry in a simplified manner and to build predictive models by parameterizing kinetic rate equations for the EM. In this procedure, the selection of a set of EM...

journal_title：Biotechnology and bioengineering

authors： Hebing L,Neymann T,Engell S

更新日期：2020-07-01 00:00:00

abstract：:A mutant of Saccharomyces cerevisiae, which forms large, multicellular flocs in liquid culture, rapidly fermented media containing high concentrations of glucose (100-180 g/L) in a continuous nonaerated tower fermentor at 30 degrees C. The fermentor operated continuously for seven months. Batch and tower fermentor dat...

journal_title：Biotechnology and bioengineering

authors： Jones ST,Korus RA,Admassu W,Heimsch RC

更新日期：1984-07-01 00:00:00

abstract：:A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...

journal_title：Biotechnology and bioengineering

authors： Shiomi N,Fukuda H,Fukuda Y,Murata K,Kimura A

更新日期：1990-05-01 00:00:00

abstract：:The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size wit...

journal_title：Biotechnology and bioengineering

authors： Chan MY,Hoare M,Dunnill P

更新日期：1986-03-01 00:00:00

abstract：:The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

journal_title：Biotechnology and bioengineering

authors： Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

更新日期：2006-08-20 00:00:00

abstract：:Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...

journal_title：Biotechnology and bioengineering

authors： Jong JZ,Hsiun DY,Wu WT,Tzeng YM

更新日期：1995-11-05 00:00:00

abstract：:The study was done to evaluate the efficacy of using reuterin produced by Lactobacillus reuteri to sanitize biological tissues. The microorganism tested in the study was Pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. The inhibitory effect of reuterin on P. aeruginosa for an inocul...

journal_title：Biotechnology and bioengineering

authors： Liang HF,Chen CN,Chang Y,Sung HW

更新日期：2003-10-20 00:00:00

abstract：:The coenzyme NAD plays a major role in metabolism as a key redox carrier and signaling molecule but current measurement techniques cannot distinguish between different compartment pools, between free and protein-bound forms and/or between NAD(H) and NADP(H). Local free NAD/NADH ratios can be determined from product/su...

journal_title：Biotechnology and bioengineering

authors： Canelas AB,van Gulik WM,Heijnen JJ

更新日期：2008-07-01 00:00:00

abstract：:To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...

journal_title：Biotechnology and bioengineering

authors： Oh MK,Kim BG,Park SH

更新日期：1995-09-20 00:00:00

abstract：:The kinetics in batch culture of the acetone butanol fermentation by Clostridium acetobutylicum is compared on glucose, xylose, and mixtures of both sugars. The fastest initial growth and transition from an acid to a solvent metabolism occurs on glucose, with a final 62 g/L glucose conversion. On xylose, an initial sl...

journal_title：Biotechnology and bioengineering

authors： Fond O,Engasser JM,Matta-El-Amouri G,Petitdemange H

更新日期：1986-02-01 00:00:00

abstract：:Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

journal_title：Biotechnology and bioengineering

authors： Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

更新日期：2006-02-05 00:00:00

abstract：:The enzymology and kinetics of tyrosine phenol lyase (TPL) from Erwinia herbicola, and tyrosine decarboxylase (TDC) from Streptococcus faecalis have been investigated for potential use in a coimmobilized multienzyme biocatalytic system for the production of dopamine. In this multienzyme biotransformation using whole c...

journal_title：Biotechnology and bioengineering

authors： Anderson WA,Moo-Young M,Legge RL

更新日期：1992-03-25 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:Although widely used in experimental and industrial situations, genetically engineered plasmids containing the lac promoter from Escherichia coli are subject to catabolite repression when grown in glucose-containing media. Several methods of overcoming this problem have been investigated by studying the expression of ...

journal_title：Biotechnology and bioengineering

authors： Warnes A,Stephenson JR,Fooks AR,Melling J,Brown MR

更新日期：1991-11-01 00:00:00

abstract：:The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory recombinant glycoprotein. The human interleukin-2 (IL-2) mutant glyc...

journal_title：Biotechnology and bioengineering

authors： Gawlitzek M,Valley U,Wagner R

更新日期：1998-03-05 00:00:00

abstract：:Centrifugal adsorption technology (CAT) is a new compact, countercurrent technology for efficient adsorption from large liquid streams by using adsorbent particles in the micrometer range. CAT seems particularly suited for the recovery of macromolecules at low concentrations, because the small particle dimensions lead...

journal_title：Biotechnology and bioengineering

authors： Peet DJ,Bisschops MA,van Hateren SH,van der Wielen LA

更新日期：2002-05-05 00:00:00

abstract：:A biotechnological process is described to remove hydrogen sulfide (H(2)S) from high-pressure natural gas and sour gases produced in the petrochemical industry. The process operates at halo-alkaline conditions and combines an aerobic sulfide-oxidizing reactor with an anaerobic sulfate (SO(4) (2-)) and thiosulfate (S(2...

journal_title：Biotechnology and bioengineering

authors： van den Bosch PL,van Beusekom OC,Buisman CJ,Janssen AJ

更新日期：2007-08-01 00:00:00

abstract：:Mathematical models to describe transcription (Arnold et al. (2001); Biotech Bioeng 72:548-561) and translation (Mehra et al. (2003); Biotech Bioeng 84:822-841) in bacteria are modified in order to improve reaction kinetics and to include the number of polymerase molecules that are active on the DNA, as well as to inc...

journal_title：Biotechnology and bioengineering

authors： Kremling A

更新日期：2007-03-01 00:00:00

abstract：:At the most fundamental level, saccharification occurs when cell wall degrading enzymes (CWDEs) diffuse, bind to and react on readily accessible cellulose fibrils. Thus, the study of the diffusive behavior of solutes into and out of cellulosic substrates is important for understanding how biomass pore size distributio...

journal_title：Biotechnology and bioengineering

authors： Yang D,Moran-Mirabal JM,Parlange JY,Walker LP

更新日期：2013-11-01 00:00:00

abstract：:Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...

journal_title：Biotechnology and bioengineering

authors： Shimizu K,Kobayashi T,Nagara A,Matsubara M

更新日期：1985-05-01 00:00:00

abstract：:This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of ...

journal_title：Biotechnology and bioengineering

authors： McMahon M,Mulcahy P

更新日期：2002-03-05 00:00:00

abstract：:An immobilized enzyme reactor has been developed for the degradation of bilirubin as a potential treatment for neonatal jaundice. It utilizes the enzyme bilirubin oxidase from Myrothecium verrucaria, which in the presence of molecular oxygen converts bilirubin to biliverdin and other products that are much less toxic ...

journal_title：Biotechnology and bioengineering

authors： Sung C,Lavin A,Klibanov AM,Langer R

更新日期：1986-10-01 00:00:00

abstract：:The fermentation kinetics of methane production from whey permeate in a packed bed immobilized cell bioreactor at mesophilic temperatures and pHs around neutral was studied. Propionate and acetate were the only two major organic intermediates found in the methanogenic fermentation of lactose. Based on this finding, a ...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Quo M

更新日期：1991-02-20 00:00:00

abstract：:Current techniques for characterizing biofilm physiology lack the signal filtering capability required for quantifying signals associated with real time biologically active transport. Though a great deal was learned from previous investigations, no results have been reported on the characterization of in vivo, real ti...

journal_title：Biotechnology and bioengineering

authors： McLamore ES,Porterfield DM,Banks MK

更新日期：2009-02-15 00:00:00

abstract：:A drug-releasing model compound based on photosensitive acrylated ortho-nitrobenzylether (o-NBE) moiety and fluorescein was synthesized to demonstrate photolysis as a mechanism for drug release. Release of this model compound from a hydrogel network can be controlled with light intensity (5-20 mW/cm(2)), exposure dura...

journal_title：Biotechnology and bioengineering

authors： Griffin DR,Patterson JT,Kasko AM

更新日期：2010-12-15 00:00:00

abstract：:The catalytic properties of bovine serum albumin (BSA) have been studied rather extensively. The protein has shown catalytic activities toward a variety of reactions in aqueous media. However, there appears to be no reports of reactions catalyzed exclusively in organic media. In this study, we report the catalytic pro...

journal_title：Biotechnology and bioengineering

authors： Liu XC,Lemma-Gray P

更新日期：2001-09-20 00:00:00