Expression of recombinant protein A from the lac promoter in Escherichia coli JM83 is not subject to catabolite repression when grown under specific conditions of continuous culture.

abstract：:Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-ga...

journal_title：Biotechnology and bioengineering

authors： Banerjee M,Chakrabarty A,Majumdar SK

更新日期：1982-08-01 00:00:00

abstract：:Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

journal_title：Biotechnology and bioengineering

authors： Kim Y,Arp DJ,Semprini L

更新日期：2002-12-05 00:00:00

abstract：:Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

journal_title：Biotechnology and bioengineering

authors： Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

更新日期：2008-01-01 00:00:00

abstract：:Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondro...

journal_title：Biotechnology and bioengineering

authors： Oh TI,Kim C,Karki B,Son Y,Lee E,Woo EJ

更新日期：2015-02-01 00:00:00

abstract：:This paper presents a new formulation for the analysis of growth kinetics on multiple nutrients. The baseline for the theory is the concept that a noninteractive growth process occurs among perfectly substitutable nutrients if the locus of points of the substrate concentrations producing equal growth rate is linear. A...

journal_title：Biotechnology and bioengineering

authors： Haas CN

更新日期：1994-06-20 00:00:00

abstract：:We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

journal_title：Biotechnology and bioengineering

authors： Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

更新日期：2017-08-01 00:00:00

abstract：:Calculation of kinetic constants of an enzymatic reaction in organic solvents requires knowledge of the functional active-site concentration in organic solvents, and this can be significantly different than that in water. An experimental method for active-site titration of serine proteases in organic media has been de...

journal_title：Biotechnology and bioengineering

authors： Wangikar PP,Carmichael D,Clark DS,Dordick JS

更新日期：1996-05-05 00:00:00

abstract：:Detachment from biofilms was evaluated using a mixed culture biofilm grown on primary wastewater in a tube reactor. The growth of biofilms and the detachment of biomass from biofilms are strongly influenced by hydrodynamic conditions. In a long-term study, three biofilms were cultivated in a biofilm tube reactor. The ...

journal_title：Biotechnology and bioengineering

authors： Horn H,Reiff H,Morgenroth E

更新日期：2003-03-05 00:00:00

abstract：:Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational quality of recombinant ...

journal_title：Biotechnology and bioengineering

authors： Martínez-Alonso M,García-Fruitós E,Villaverde A

更新日期：2008-12-15 00:00:00

abstract：:The emergence of medicinal indications for stem cell therapies has seen a need to develop the manufacturing capacity for adherent cells such as mesenchymal stem cells (MSCs). One such development is in the use of microcarriers, which facilitate enhanced cell densities for adherent stem cell cultures when compared with...

journal_title：Biotechnology and bioengineering

authors： Odeleye AOO,Castillo-Avila S,Boon M,Martin H,Coopman K

更新日期：2017-09-01 00:00:00

abstract：:Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

journal_title：Biotechnology and bioengineering

authors： Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

更新日期：2019-01-01 00:00:00

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:Installing membranes for solid-liquid separation into biological nutrient removal (BNR) activated sludge (AS) systems makes a profound difference not only in the design of the BNR system itself, but also in the design approach for the whole wastewater treatment plant (WWTP). In multizone BNR systems with membranes in ...

journal_title：Biotechnology and bioengineering

authors： Ramphao M,Wentzel MC,Merritt R,Ekama GA,Young T,Buckley CA

更新日期：2005-03-20 00:00:00

abstract：:The major objectives for preparative protein chromatography are maximal loading and increased flow rate while maintaining defined resolution. Conventionally a series of chromatographic experiments are performed and the optimal conditions are selected according to the separation criteria. Computer-aided process design ...

journal_title：Biotechnology and bioengineering

authors： Jungbauer A,Kaltenbrunner O

更新日期：1996-10-20 00:00:00

abstract：:In biotechnology and biomedicine reliable models of cell proliferation kinetics need to capture the relevant phenomena taking place during the mitotic cycle. To this aim, a novel mathematical model helpful to investigate the intrinsic kinetics of in vitro culture of adherent cells up to confluence is proposed in this ...

journal_title：Biotechnology and bioengineering

authors： Fadda S,Cincotti A,Cao G

更新日期：2012-03-01 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:In this report, alpha-Amylase originating from Bacillus subtilis (liquefying type) was immobilized on partially imidoesterized polyacrylonitrile (PAN) by covalent bonding. For the preparation of immobilized alpha-amylase, which has a high activity and high stability to repeated use, the optimum conditions for the prep...

journal_title：Biotechnology and bioengineering

authors： Handa T,Hirose A,Akino T,Watanabe K,Tsuchiya H

更新日期：1983-12-01 00:00:00

abstract：:Alcaligenes Eutrophus H-16 was grown in continuous culture under conditions which induced hydrogenase production. The hydrogenase enzyme was extracted, partially purified and immobilized on porous glass. This enzyme was then studied both in solution and in immobilized form as a possible candidate for a number of indus...

journal_title：Biotechnology and bioengineering

authors： Deloggio TJ,Graves DJ

更新日期：1988-07-20 00:00:00

abstract：:The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradation of PAHs in soils can be enhanced by employing surface-active agents. However, chemical surfactants are often recalcitrant and exer...

journal_title：Biotechnology and bioengineering

authors： Fava F,Berselli S,Conte P,Piccolo A,Marchetti L

更新日期：2004-10-20 00:00:00

abstract：:The fermentation kinetics of methane production from whey permeate in a packed bed immobilized cell bioreactor at mesophilic temperatures and pHs around neutral was studied. Propionate and acetate were the only two major organic intermediates found in the methanogenic fermentation of lactose. Based on this finding, a ...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Quo M

更新日期：1991-02-20 00:00:00

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:Small interfering RNAs (siRNAs) are invaluable research tools for studying gene functions in mammalian cells. siRNAs are mainly produced by chemical synthesis or by enzymatic digestion of double-stranded RNA (dsRNA) produced in vitro. Recently, bacterial cells, engineered with ectopic plant viral siRNA binding protein...

journal_title：Biotechnology and bioengineering

authors： Kaur G,Cheung HC,Xu W,Wong JV,Chan FF,Li Y,McReynolds L,Huang L

更新日期：2018-09-01 00:00:00

abstract：:Autoflocculation and bioflocculation are considered to be the most promising means for the economical harvesting of microalgae. We have therefore studied these phenomena with cultures of Scenedesmus obliquus produced during biological tertiary wastewater treatment. The quantity of extracellular polymers produced durin...

journal_title：Biotechnology and bioengineering

authors： Lavoie A,de la Noüe J

更新日期：1987-11-01 00:00:00

abstract：:The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...

journal_title：Biotechnology and bioengineering

authors： Beun JJ,Heijnen JJ,van Loosdrecht MC

更新日期：2001-10-05 00:00:00

abstract：:Direct perfusion of 3D tissue engineered constructs is known to enhance osteogenesis, which can be partly attributed to enhanced nutrient and waste transport. In addition flow mediated shear stresses are known to upregulate osteogenic differentiation and mineralization. A quantification of the hydrodynamic environment...

journal_title：Biotechnology and bioengineering

authors： Maes F,Van Ransbeeck P,Van Oosterwyck H,Verdonck P

更新日期：2009-06-15 00:00:00

abstract：:The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The value...

journal_title：Biotechnology and bioengineering

authors： Sokol W

更新日期：1987-12-05 00:00:00

abstract：:A new method for solid-state ethanol fermentation (the SSEF system) was experimented on for the ethanol production from solid starchy materials, where a packedbed-type fermentor was used. Both cultivation of Aspergillus saitoi and enrichment of a saccharifying enzyme were effective for hydrolysis of the starch. Ethano...

journal_title：Biotechnology and bioengineering

authors： Sato K,Nakamura K,Sato S

更新日期：1985-09-01 00:00:00

abstract：:A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...

journal_title：Biotechnology and bioengineering

authors： Feins M,Sirkar KK

更新日期：2004-06-20 00:00:00

abstract：:Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis ...

journal_title：Biotechnology and bioengineering

authors： Sivakumar G,Liu C,Towler MJ,Weathers PJ

更新日期：2010-12-01 00:00:00

abstract：:Seven different supports were compared in solid-phase S(N)Ar and S(N)2 macrocyclization reactions. Product purities were assayed for a relatively facile ring-closure process to give products 1 and 3. Some less-facile ring-closure reactions give the undesired dimeric macrocyclization by-products 2; some of these more-d...

journal_title：Biotechnology and bioengineering

authors： Feng Y,Burgess K

更新日期：2000-01-01 00:00:00