Oxidation of an inhibitory substrate by washed cells (oxidation of phenol by Pseudomonas putida).

abstract：:Trichoplusia ni larvae were infected with baculoviruses containing genes for the expression of ultraviolet optimized green fluorescent protein (GFPuv) and several product proteins. A GFP-specific optical probe was used to both excite the green fluorescent protein (lambda(ex) = 385 nm), and subsequently monitor fluores...

journal_title：Biotechnology and bioengineering

authors： Kramer SF,Kostov Y,Rao G,Bentley WE

更新日期：2003-07-20 00:00:00

abstract：:A production process for B. thuringiensis (Bt) bioinsecticides was designed in detail, including alternative batch, low-density fed-batch (LDFB), and high-density fed-batch (HDFB) fermentation configurations. Capital and operating costs, as well as profitability based on simple rate of return, were performed using a p...

journal_title：Biotechnology and bioengineering

authors： Rowe GE,Margaritis A

更新日期：2004-05-20 00:00:00

abstract：:In a bioelectrochemical system (BES), microbial community of anode biofilm is crucial to BES performance. In this study, the stratified pattern of community structure and activity of an anode-respiring biofilm in a BES fueled with brewery wastewater was investigated over time. The anode biofilm exhibited a superior pe...

journal_title：Biotechnology and bioengineering

authors： Mai Q,Yang G,Cao J,Zhang X,Zhuang L

更新日期：2020-07-01 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...

journal_title：Biotechnology and bioengineering

authors： Prokop A,Ratcliffe HD,Fatayer MI,Al-Awadhi N,Khamis A,Murad M,Bond C,Hamdan IY

更新日期：1984-09-01 00:00:00

abstract：:Several researchers have demonstrated that the presence of a heterologous protein in recombinant Escherichia coli elicits a response similar to the heat-shock response, which includes enhanced protease expression. The present work detects, quantifies, and characterizes intracellular protease activity in E. coli that a...

journal_title：Biotechnology and bioengineering

authors： Harcum SW,Bentley WE

更新日期：1993-09-05 00:00:00

abstract：:The budding yeast Saccharomyces cerevisiae is equipped with G protein-coupled receptors (GPCR). Because the yeast GPCR signaling mechanism is partly similar to that of the mammalian system, S. cerevisiae can be used for a host of mammalian GPCR expression and ligand-mediated activation assays. However, currently avail...

journal_title：Biotechnology and bioengineering

authors： Fukutani Y,Ishii J,Kondo A,Ozawa T,Matsunami H,Yohda M

更新日期：2017-06-01 00:00:00

abstract：:Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

journal_title：Biotechnology and bioengineering

authors： Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

更新日期：1993-07-01 00:00:00

abstract：:The regeneration of functional, clinically viable, tissues from acellular ex vivo tissues has been problematic largely due to poor nutrient transport conditions that limit cell migration and integration. Compounding these issues are subcellular pore sizes that necessarily requires extracellular matrix (ECM) remodeling...

journal_title：Biotechnology and bioengineering

authors： Tosun Z,McFetridge PS

更新日期：2013-07-01 00:00:00

abstract：:Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied in wastewater-fed M...

journal_title：Biotechnology and bioengineering

authors： Bourdakos N,Marsili E,Mahadevan R

更新日期：2014-04-01 00:00:00

abstract：:For the application of immobilized enzymes, fixed bed reactors are used almost exclusively. Fixed bed reactors have specific disadvantages, especially for processes with a deactivating catalyst. Therefore, we have studied a novel reactor type with continuous transport of the immobilized biocatalyst. Flow of biocatalys...

journal_title：Biotechnology and bioengineering

authors： Vos HJ,Groen DJ,Potters JJ,Luyben KC

更新日期：1990-08-05 00:00:00

abstract：:An integrated approach is used to develop a rapid sampling strategy for the quantitative analysis of in vivo kinetic behavior based on measured concentrations of intracellular metabolites in Saccharomyces cerevisiae. Emphasis is laid on small sample sizes during sampling and analysis. Subsecond residence times are acc...

journal_title：Biotechnology and bioengineering

authors： Lange HC,Eman M,van Zuijlen G,Visser D,van Dam JC,Frank J,de Mattos MJ,Heijnen JJ

更新日期：2001-11-20 00:00:00

abstract：:Centrifugal adsorption technology (CAT) is a new compact, countercurrent technology for efficient adsorption from large liquid streams by using adsorbent particles in the micrometer range. CAT seems particularly suited for the recovery of macromolecules at low concentrations, because the small particle dimensions lead...

journal_title：Biotechnology and bioengineering

authors： Peet DJ,Bisschops MA,van Hateren SH,van der Wielen LA

更新日期：2002-05-05 00:00:00

abstract：:Oxygenases catalyze, among other interesting reactions, highly selective hydrocarbon oxyfunctionalizations, which are important in industrial organic synthesis but difficult to achieve by chemical means. Many enzymatic oxygenations have been described, but few of these have been scaled up to industrial scales, due to ...

journal_title：Biotechnology and bioengineering

authors： Bühler B,Bollhalder I,Hauer B,Witholt B,Schmid A

更新日期：2003-06-30 00:00:00

abstract：:Expanded bed adsorption is a new downstream processing technique forcapture of proteins directly from unclarified feedstocks. Expanded bed adsorption reduces the number of operations in purification processes by combining clarification, concentration, and capture into one operation. It is based on stable fluidization ...

journal_title：Biotechnology and bioengineering

authors： Barnfield Frej AK,Hjorth R,Hammarström A

更新日期：1994-10-01 00:00:00

abstract：:Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

journal_title：Biotechnology and bioengineering

authors： Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

更新日期：2008-07-01 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially incr...

journal_title：Biotechnology and bioengineering

authors： Jones EO,Lee JM

更新日期：1988-01-01 00:00:00

abstract：:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

journal_title：Biotechnology and bioengineering

authors： Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

更新日期：1997-06-05 00:00:00

abstract：:Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...

journal_title：Biotechnology and bioengineering

authors： Zhao L,Ye B,Zhang Q,Cheng D,Zhou C,Cheng S,Yan X

更新日期：2019-08-01 00:00:00

abstract：:Peroxidase-catalyzed asymmetric sulfoxidations, while synthetically attractive, suffer from relatively low reaction rates due to poor substrate solubilities in water and from appreciable spontaneous oxidation of substrates (especially aryl alkyl sulfides) with H(2)O(2). In this work, we found that both of these shortc...

journal_title：Biotechnology and bioengineering

authors： Dai L,Klibanov AM

更新日期：2000-11-05 00:00:00

abstract：:The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...

journal_title：Biotechnology and bioengineering

authors： Hortacsu A,Ryu DD

更新日期：1991-10-20 00:00:00

abstract：:At the most fundamental level, saccharification occurs when cell wall degrading enzymes (CWDEs) diffuse, bind to and react on readily accessible cellulose fibrils. Thus, the study of the diffusive behavior of solutes into and out of cellulosic substrates is important for understanding how biomass pore size distributio...

journal_title：Biotechnology and bioengineering

authors： Yang D,Moran-Mirabal JM,Parlange JY,Walker LP

更新日期：2013-11-01 00:00:00

abstract：:Escherichia coli has been the host organism most frequently investigated for efficient recombinant protein production. However, the production of a foreign protein in recombinant E. coli often leads to growth deterioration and elevated secretion of acetic acid. Such observed phenomena have been widely linked with cell...

journal_title：Biotechnology and bioengineering

authors： Zeng H,Yang A

更新日期：2019-06-01 00:00:00

abstract：:It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

journal_title：Biotechnology and bioengineering

authors： Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

更新日期：2008-05-01 00:00:00

abstract：:The use of expanded beds of STREAMLINE ion exchange adsorbents for the direct extraction of an intracellular enzyme glucose-6-phosphate dehydrogenase (G6PDH) from unclarified yeast cell homogenates has been investigated. It has been demonstrated that such crude feedstocks can be applied to the bed without prior clarif...

journal_title：Biotechnology and bioengineering

authors： Chang YK,Chase HA

更新日期：1996-01-20 00:00:00

abstract：:Biofilms commonly develop in flowing aqueous environments, where the flow causes the biofilm to deform. Because biofilm deformation affects the flow regime, and because biofilms behave as complex heterogeneous viscoelastic materials, few models are able to predict biofilm deformation. In this study, a phase-field (PF)...

journal_title：Biotechnology and bioengineering

authors： Li M,Matouš K,Nerenberg R

更新日期：2020-07-13 00:00:00

abstract：:In our preceding article, we demonstrated a procedure based upon enzymic flow microcalorimetry using an enzyme thermistor (ET) to characterize the microkinetic properties of an immobilized enzyme (IME) and its further application in the screening of IMEs. To consider the ET method (single ET unit, ET system 1) as stan...

journal_title：Biotechnology and bioengineering

authors： Gemeiner P,Docolomansky P,Nahálka J,Stefuca V,Danielsson B

更新日期：1996-01-05 00:00:00

abstract：:The stoichiometry and kinetics of the spontaneous, chemical reaction between pyrite and ferric iron was studied at 30, 45, and 70 degrees C in shake flasks at pH 1.5 by monitoring the ferrous iron, total iron, elemental sulfur, and sulfate concentration profiles in time. It was found that the sulfur moiety of pyrite w...

journal_title：Biotechnology and bioengineering

authors： Boogerd FC,van den Beemd C,Stoelwinder T,Bos P,Kuenen JG

更新日期：1991-06-20 00:00:00

abstract：:The fim inversion system of Escherichia coli (E. coli) can behave as a unidirectional switch in an efficient manner. We have developed a new expression system for E. coli, comprising the arabinose-inducible fimE gene and the fim invertible DNA segment containing a constitutively active promoter. In this system, the ta...

journal_title：Biotechnology and bioengineering

authors： Ham TS,Lee SK,Keasling JD,Arkin AP

更新日期：2006-05-05 00:00:00