Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption.

abstract：:Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTO...

journal_title：Biotechnology and bioengineering

authors： Kim MH,Wang N,McDonald T,Chu KH

更新日期：2012-12-01 00:00:00

abstract：:The denitrification capability of Cluster 1 Defluviicoccus vanus-related glycogen-accumulating organisms (DvGAOs) is investigated. A sequencing batch reactor (SBR) fed with acetate as the sole carbon source was operated under alternating anaerobic-aerobic conditions to enrich Cluster 1 DvGAOs. Fluorescence in situ hyb...

journal_title：Biotechnology and bioengineering

authors： Wang X,Zeng RJ,Dai Y,Peng Y,Yuan Z

更新日期：2008-04-15 00:00:00

abstract：:The morphology of mycelial fungi in liquid culture effects culture rheology and this in turn may affect product yield. It is therefore important to understand how environmental factors influence mycelial morphology and this paper describes the effect of dilution rate on two strains of Fusarium graminearum, the relativ...

journal_title：Biotechnology and bioengineering

authors： Wiebe MG,Trinci AP

更新日期：1991-06-05 00:00:00

abstract：:The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponen...

journal_title：Biotechnology and bioengineering

authors： Zheng ZM,Xu YZ,Liu HJ,Guo NN,Cai ZZ,Liu DH

更新日期：2008-08-01 00:00:00

abstract：:A mechanistic model-based soft sensor is developed and validated for 550L filamentous fungus fermentations operated at Novozymes A/S. The soft sensor is comprised of a parameter estimation block based on a stoichiometric balance, coupled to a dynamic process model. The on-line parameter estimation block models the cha...

journal_title：Biotechnology and bioengineering

authors： Mears L,Stocks SM,Albaek MO,Sin G,Gernaey KV

更新日期：2017-03-01 00:00:00

abstract：:The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...

journal_title：Biotechnology and bioengineering

authors： Chung R,Kalyon DM,Yu X,Valdevit A

更新日期：2018-09-01 00:00:00

abstract：:The purpose of this study was to understand bacteria profile modification and its applications in subsurface biological operations such as biobarrier formation, in situ bioremediation, and microbial-enhanced oil recovery. Biomass accumulation and evolution in porous media were investigated both experimentally and theo...

journal_title：Biotechnology and bioengineering

authors： Kim DS,Fogler HS

更新日期：2000-07-05 00:00:00

abstract：:Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...

journal_title：Biotechnology and bioengineering

authors： Soriano E,Borth N,Katinger H,Mattanovich D

更新日期：2002-10-05 00:00:00

abstract：:A major challenge when designing cell scaffolds for chondrocyte delivery in vivo is creating scaffolds with sufficient mechanical properties to restore initial function while simultaneously controlling temporal changes in the gel structure to facilitate tissue formation. To address this design challenge, degradable ph...

journal_title：Biotechnology and bioengineering

authors： Bryant SJ,Bender RJ,Durand KL,Anseth KS

更新日期：2004-06-30 00:00:00

abstract：:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...

journal_title：Biotechnology and bioengineering

authors： López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano G

更新日期：2017-03-01 00:00:00

abstract：:The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...

journal_title：Biotechnology and bioengineering

authors： Janseen AE,Van der Padt A,Van Sonsbeek HM,Van't Riet K

更新日期：1993-01-05 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:Flow cytometry is a fast and sensitive method that allows monitoring of different cellular parameters on large samples of a population. Protein distributions give relevant information on growth dynamics, since they are related to the age distribution and depend on the law of growth of the population and the law of pro...

journal_title：Biotechnology and bioengineering

authors： Alberghina L,Mariani L,Martegani E,Vanoni M

更新日期：1983-05-01 00:00:00

abstract：:A mixed culture of sulfate-reducing bacteria containing the species Desulfovibrio desulfuricans was used to study sulfate-reduction stoichiometry and kinetics using ethanol as the carbon source. Growth yield was lower, and kinetics were slower, for ethanol compared to lactate. Ethanol was converted into acetate and no...

journal_title：Biotechnology and bioengineering

authors： Nagpal S,Chuichulcherm S,Livingston A,Peeva L

更新日期：2000-12-05 00:00:00

abstract：:A facile methodology has been developed for covalently derivatizing the surfaces of common materials with a designed antibacterial polycation, poly(vinyl-N-pyridinium bromide), wherein the first, key step involves surface coating with a nanolayer of silica. Various commercial synthetic polymers derivatized in this man...

journal_title：Biotechnology and bioengineering

authors： Tiller JC,Lee SB,Lewis K,Klibanov AM

更新日期：2002-08-20 00:00:00

abstract：:Microfluidic bioreactors have been shown valuable for various cellular applications. The use of micro-wells/grooves bioreactors, in which micro-topographical features are used to protect sensitive cells from the detrimental effects of fluidic shear stress, is a promising approach to culture sensitive cells in these pe...

journal_title：Biotechnology and bioengineering

authors： Korin N,Bransky A,Khoury M,Dinnar U,Levenberg S

更新日期：2009-03-01 00:00:00

abstract：:The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as substrate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL B-512F dextransucras...

journal_title：Biotechnology and bioengineering

authors： Dols M,Remaud-Simeon M,Willemot RM,Demuth B,Jordening HJ,Buchholz K,Monsan P

更新日期：1999-05-05 00:00:00

abstract：:The application of enantioseparation methods alone can only yield up to 50% of the desired chiral product. Thus enantioseparation becomes more attractive when accompanied by the racemization of the counter-enantiomer. Here we present first results of dynamic kinetic resolution of L-asparagine (L-Asn) via preferential ...

journal_title：Biotechnology and bioengineering

authors： Würges K,Petrusevska-Seebach K,Elsner MP,Lütz S

更新日期：2009-12-15 00:00:00

abstract：:Hexanal is a key organoleptic element of green-note that is found in both fragrances and flavors. We report a novel process for the production of hexanal using immobilized enzyme templates extracted from different plant sources in combination with hollow-fiber ultrafiltration for in situ separation. Enzyme templates, ...

journal_title：Biotechnology and bioengineering

authors： Schade F,Thompson JE,Legge RL

更新日期：2003-11-05 00:00:00

abstract：:Dehydrogenase activity assay of activated sludge using the redox dye 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) was investigated. INT-dehydrogenase activity (INT-DHA) was directly proportional to INT dosage and inversely proportional to bio-mass concentration over limited ranges. INT dosages...

journal_title：Biotechnology and bioengineering

authors： Lopez JM,Koopman B,Bitton G

更新日期：1986-07-01 00:00:00

abstract：:The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...

journal_title：Biotechnology and bioengineering

authors： Yuan H,Liu Y,Li J,Shin HD,Du G,Shi Z,Chen J,Liu L

更新日期：2018-09-01 00:00:00

abstract：:A thermodynamic model was used in this study to predict the adhesion of Phanerochaete chrysosporium INA-12 as conidiospores or mycelium to various solid carriers. Theoretical predictions were closely reflected by experimental results. Amount of immobilized mycelium was higher for hydrophobic (polypropylene and polyure...

journal_title：Biotechnology and bioengineering

authors： Asther M,Bellon-Fontaine MN,Capdevila C,Corrieu G

更新日期：1990-03-05 00:00:00

abstract：:Differences in plasmid retention and expression are studied in both suspended and biofilm cultures of Escherichia coli DH5alpha(PMJR1750). An alternative mathematical model is proposed which allows the determination of plasmid loss probability in both suspended batch and continuously fed biofilm cultures. In our exper...

journal_title：Biotechnology and bioengineering

authors： Huang CT,Peretti SW,Bryers JD

更新日期：1993-01-20 00:00:00

abstract：:Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves o...

journal_title：Biotechnology and bioengineering

authors： Stieglitz B,Defelice CP

更新日期：1986-09-01 00:00:00

abstract：:The key objective for recombinant protein production in bacteria is the maximum exploitation of the cell factory's potential, whereby often strong expression vectors are used to increase product yield. If the metabolic load caused by recombinant expression exceeds the host's capacity, the system exhausts itself, resul...

journal_title：Biotechnology and bioengineering

authors： Grabherr R,Nilsson E,Striedner G,Bayer K

更新日期：2002-01-20 00:00:00

abstract：:Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

journal_title：Biotechnology and bioengineering

authors： Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

更新日期：2019-01-01 00:00:00

abstract：:The feasibility of producing ethanol in a continuous system from cellulose using Clostridirrrn thermocellum was investigated. This anaerobic and therniophilic bacterium was able to degrade cellulose directly into ethanol with acetic acid, hydrogen. and carbon dioxide as by-products of this fermentation. The fermentati...

journal_title：Biotechnology and bioengineering

authors： Zertuche L,Zall RR

更新日期：1982-01-01 00:00:00

abstract：:Several synthetic metabolic pathways for butanol synthesis have been reported in Escherichia coli by modification of the native CoA-dependent pathway from selected Clostridium species. These pathways are all dependent on the O2 -sensitive AdhE2 enzyme from Clostridium acetobutylicum that catalyzes the sequential reduc...

journal_title：Biotechnology and bioengineering

authors： Pásztor A,Kallio P,Malatinszky D,Akhtar MK,Jones PR

更新日期：2015-01-01 00:00:00

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...

journal_title：Biotechnology and bioengineering

authors： Brethauer S,Held M,Panke S

更新日期：2008-06-15 00:00:00