Abstract:
:Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves of number of areas or wells inoculated versus cell concentration were prepared for bacteria, yeast and fungi (spores). Experiments with yeast auxotrophic mutants and fungal spores showed that microbe inoculation follows Poisson statistics in distributing a single microorganism per inoculation point. The isolation and identification of Yarrowia lipolytica auxotrophic, morphological, and temperature-sensitive or tolerant mutants demonstrated the use of the instruments for microbial screening.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Stieglitz B,Defelice CPdoi
10.1002/bit.260280905subject
Has Abstractpub_date
1986-09-01 00:00:00pages
1310-7issue
9eissn
0006-3592issn
1097-0290journal_volume
28pub_type
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20777
更新日期:2006-04-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280107
更新日期:1986-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20484
更新日期:2005-07-05 00:00:00
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doi:10.1002/bit.21274
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更新日期:1987-04-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260441114
更新日期:1994-12-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24803
更新日期:2013-05-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-12-05 00:00:00
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更新日期:2003-08-20 00:00:00
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更新日期:2013-03-01 00:00:00
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更新日期:2007-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-12-15 00:00:00