Abstract：
:The key objective for recombinant protein production in bacteria is the maximum exploitation of the cell factory's potential, whereby often strong expression vectors are used to increase product yield. If the metabolic load caused by recombinant expression exceeds the host's capacity, the system exhausts itself, resulting in a loss of protein yield. Excessive plasmid replication is observed after inducing recombinant gene expression, which greatly contributes to metabolic overload of the host cell. The transcriptional and translational machineries are extremely overstrained. By abolishing sequence homology between ColE1 RNA I/RNA II and tRNAs, we were able to restore the plasmid's replication control mechanisms and to keep the plasmid copy number constant throughout the culture process, thereby prolonging metabolic activity and productivity of the bacterial expression system. Because the bacterial host cell is not being exploited beyond its tolerable potential with this method, the constancy of the plasmid copy number level throughout the whole period of the bioprocess provides novel strategies for bioprocess optimization.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Grabherr R,Nilsson E,Striedner G,Bayer Kdoi
10.1002/bit.10104subject
Has Abstractpub_date
2002-01-20 00:00:00pages
142-7issue
2eissn
0006-3592issn
1097-0290pii
10.1002/bit.10104journal_volume
77pub_type
杂志文章abstract：:Bamboo carbohydrates were hydrolyzed with commercial amylases and a mixture of fungal culture broths containing cellulolytic and hemicellulolytic enzymes. The effects of cooking temperature and the size of fiber particles were also investigated. It was found that the higher the cooking temperature, the higher the rate...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260250415
更新日期：1983-04-01 00:00:00
abstract：:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especia...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260290707
更新日期：1987-05-01 00:00:00
abstract：:This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-co...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.26030
更新日期：2016-12-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.27277
更新日期：2020-04-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260280312
更新日期：1986-03-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.22704
更新日期：2010-06-15 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260410103
更新日期：1993-01-05 00:00:00
abstract：:A bioremediation process to remove Co(2+) from aqueous solution is investigated in this study using a magnetic resonance imaging (MRI) protocol to rapidly obtain multiple 2D spatially resolved Co(2+) ion concentration maps. The MRI technique is described in detail and its ability to determine the evolution in both axi...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.21647
更新日期：2008-03-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20369
更新日期：2005-02-20 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260281110
更新日期：1986-11-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/(sici)1097-0290(19990420)63:2<141::aid-bit
更新日期：1999-04-20 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20046
更新日期：2004-05-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：
更新日期：2000-11-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260270803
更新日期：1985-08-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260260509
更新日期：1984-05-01 00:00:00
abstract：:While feeding protocols and ectopic expression of anti-apoptotic genes have been used to improve the viability of hybridoma cell lines, the effect of the expression levels of survival genes on the behavior of hybridomas following nutrient supplementation is unknown. In this study, we compared the behavior of the Sp2/0...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.10467
更新日期：2003-02-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.21421
更新日期：2007-09-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/(SICI)1097-0290(19961220)52:6<713::AID-BIT
更新日期：1996-12-20 00:00:00
abstract：:It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20120
更新日期：2004-08-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260380510
更新日期：1991-08-20 00:00:00
abstract：:For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20435
更新日期：2005-05-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260440113
更新日期：1994-06-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260460204
更新日期：1995-04-20 00:00:00
abstract：:Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/(sici)1097-0290(20000220)67:4<451::aid-bit
更新日期：2000-02-20 00:00:00
abstract：:Sugarcane bagasse is an abundant and sustainable resource, generated as a by-product of sugarcane milling. The cellulosic material within bagasse can be broken down into glucose molecules and fermented to produce ethanol, making it a promising feedstock for biofuel production. Mild acid pretreatment hydrolyses the hem...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.24984
更新日期：2013-12-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20399
更新日期：2005-04-20 00:00:00
abstract：:A stimulus-response tracer technique has been used to characterize packed beds of untreated, as well as acid prehydrolyzed, and enzymatically hydrolyzed aspen wood chips. Glucose was used as the tracer. Bulk liquid phase dispersion, interphase mass transfer, and intraparticle diffusion coefficients were determined for...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260410310
更新日期：1993-02-05 00:00:00
abstract：:A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.22242
更新日期：2009-06-01 00:00:00
abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260170802
更新日期：1975-08-01 00:00:00
abstract：:Stricter regulations on volatile organic compounds and hazardous air pollutants have increased the demand for abatement technologies. Biofiltration, a process in which contaminated air is passed through a biologically active bed, can be used to remove these pollutants from air streams. In this study, a fungal vapor-ph...
journal_title：Biotechnology and bioengineering
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doi：10.1002/bit.10066
更新日期：2001-12-05 00:00:00
