Abstract:
:Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusion could explain the specific rate of glucose uptake observed during the production phase in several different types of fermentation.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Wayman FM,Mattey Mdoi
10.1002/(sici)1097-0290(20000220)67:4<451::aid-bitsubject
Has Abstractpub_date
2000-02-20 00:00:00pages
451-6issue
4eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(20000220)67:4<451::AID-BITjournal_volume
67pub_type
杂志文章abstract::The production of recombinant proteins by mammalian cells demands a highly controlled environment for cell cultivation. Temperature stress represents a commonly encountered disturbance in both research and process environments. In this study, we examined the effects of heat shock on the expression of recombinant human...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260401008
更新日期:1992-12-05 00:00:00
abstract::Many bacteria used for biotechnological applications are naturally motile. Their "bio-nanopropeller" driven movement allows searching for better environments in a process called chemotaxis. Since bacteria are extremely small in size compared to the bulk fluid volumes in bioreactors, single cell motility is not conside...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21372
更新日期:2007-08-15 00:00:00
abstract::Point and 95% confidence interval estimates of the true growth yield and maintenance coefficient are reported for four pure cultures andtwo mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus grown anaerobically on 3% nonfat dry milk using batch followed by fed batch culture. An improved method o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280619
更新日期:1986-06-01 00:00:00
abstract::One of the critical problems in scale-up of affinity chromatography is the mechanical strength of the support matrix against pressure. Because the costs of both the gel matrix and the ligand for the affinity chromatography are very high, the reusability of gel matrices is directly related to the total production cost....
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260391104
更新日期:1992-05-01 00:00:00
abstract::Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21420
更新日期:2007-09-01 00:00:00
abstract::The cellulase production from immobilized Trichoderma reesei composites prepared by radiation polymerization at low temperature was studied. The production of cellulase from the cells irradiated by radiation was slightly retarded at the initial stage of the culture, but was immediately recovered. The production of cel...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260105
更新日期:1984-01-01 00:00:00
abstract::All bacteria release organic compounds called soluble microbial products (SMP) as a part of their normal metabolism. In photobioreactor (PBR) settings, SMP produced by cyanobacteria represent a major pool of carbon and electrons available to heterotrophic bacteria. Thus, SMP in PBRs are a major driver for the growth o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25602
更新日期:2015-09-01 00:00:00
abstract::This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K(s) and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm r...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260281120
更新日期:1986-11-01 00:00:00
abstract::Start-up phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. In this study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22518
更新日期:2010-01-01 00:00:00
abstract::A uniquely simple model is developed to describe the NO(3) (-) and NO(2) (-) concentration profiles within a dentrification fluidized bed biological reactor. This simple model is compared to experimental data, and to a more complex model similar to those previously proposed in the literature. The simple model fits the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970620)54:6<543::AID-BIT
更新日期:1997-06-20 00:00:00
abstract::Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10397
更新日期:2002-12-05 00:00:00
abstract::The ferrous-iron oxidation kinetics of a bacterial culture consisting predominantly of Leptospirillum ferrooxidans were studied in continuous-flow bioreactors. The bacterial culture was fed with a salts solution containing 12 g/L ferrous-iron, at dilution rates ranging from 0.01 to 0.06 l/h, and temperatures ranging f...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19991005)65:1<44::aid-bit6
更新日期:1999-10-05 00:00:00
abstract::The twin arginine translocation (Tat) pathway occurs naturally in E. coli and has the distinct ability to translocate folded proteins across the inner membrane of the cell. It has the potential to export commercially useful proteins that cannot be exported by the ubiquitous Sec pathway. To better understand the biopro...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24384
更新日期:2012-04-01 00:00:00
abstract::Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970705)55:1<118::AID-BIT
更新日期:1997-07-05 00:00:00
abstract::Metabolite accumulation has pleiotropic, toxic, or beneficial effects on cell physiology, but such effects are not well understood at the molecular level. Cells respond and adapt to metabolite stress by mechanisms largely unexplored, especially in the context of multiple and simultaneous stresses. Solventogenic and re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22628
更新日期:2010-04-15 00:00:00
abstract::Engineered human T-cells are a promising therapeutic modality for cancer immunotherapy. T-cells expressing chimeric antigen receptors combined with additional genes to enhance T-cell proliferation, survival, or tumor targeting may further improve efficacy but require multiple stable gene transfer events. Methods are t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25538
更新日期:2015-07-01 00:00:00
abstract::Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400609
更新日期:1992-09-01 00:00:00
abstract::We present a mathematical model which includes TNF-alpha initiated survival and apoptotic cascades, as well as nuclear transcription of IkappaB. These pathways play a crucial role in deciding cell fate in response to inflammation and infection. Our model incorporates known specific protein-protein interactions as iden...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21307
更新日期:2007-08-01 00:00:00
abstract::The simultaneous saccharification and co-fermentation (SSCF) kinetic model described in the companion paper can predict batch and fed batch fermentations well at solids concentrations up to 62.4 g/L cellulose paper sludge but not in batch fermentation at 82.0 g/L cellulose paper sludge. Four hypotheses for the discrep...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22465
更新日期:2009-12-01 00:00:00
abstract::Based on the recently constructed Escherichia coli itaconic acid production strain ita23, we aimed to improve the productivity by applying a two-stage process strategy with decoupled production of biomass and itaconic acid. We constructed a strain ita32 (MG1655 ΔaceA Δpta ΔpykF ΔpykA pCadCs), which, in contrast to ita...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26446
更新日期:2018-01-01 00:00:00
abstract::Bioprocess identification starts with collection of process information. Usually there is a variety of information available, consisting of actual measurement data, historical data, empirical kinetic and yield correlations, and general knowledge available from literature. A central problem is to find out how the vario...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960220)49:4<364::AID-BIT
更新日期:1996-02-20 00:00:00
abstract::Studies have been conducted in a gas circulation type fermentor in order to characterize the ethanol fermentation of uncooked cassava starch with Rhizopus koji. Results showed that ethanol concentration reached 13-14% (v/v) in 4-day broth, and the maximum productivity of ethanol was 2.3 g ethanol/L broth h. This produ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270823
更新日期:1985-08-01 00:00:00
abstract::After injury to the central nervous system, a glial scar develops that physically and biochemically inhibits axon growth. In the scar, activated astrocytes secrete inhibitory extracellular matrix, of which chondroitin sulfate proteoglycans (CSPGs) are considered the major inhibitory component. An inhibitory interface ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24750
更新日期:2013-03-01 00:00:00
abstract::The effective diffusivity of oxygen, D(e), in Ca-alginate and PVA-SbQ gels was measured using a two-chamber vessel with a membrane between the two chambers. The effect of cell density, C(c), on D(e) in Ca-alginate gels was studied. The effective diffusivity of oxygen decreased with increasing cell density, to C(c) = 1...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340107
更新日期:1989-06-05 00:00:00
abstract::The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20365
更新日期:2005-03-20 00:00:00
abstract::The performance of an industrial pharmaceutical process (production of an active pharmaceutical ingredient by fermentation, API) was modeled by multiblock partial least squares (MBPLS). The most important process stages are inoculum production and API production fermentation. Thirty batches (runs) were produced accord...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10382
更新日期:2002-11-20 00:00:00
abstract::This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21674
更新日期:2008-04-01 00:00:00
abstract::A pure culture of Pseudomonas fluorescens was used as a model system to study the kinetics of denitrification. An exponentially growing culture was harvested and resuspended in an anoxic acetate solution buffered with K/Na phosphate at pH values of 6.6, 7.0, 7.4, and 7.8. The temperature was kept at 28 degrees C in al...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260460512
更新日期:1995-06-05 00:00:00
abstract::A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340409
更新日期:1989-08-05 00:00:00
abstract::With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25589
更新日期:2015-09-01 00:00:00