Fibrin gels engineered with pro-angiogenic growth factors promote engraftment of pancreatic islets in extrahepatic sites in mice.


:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin domain. Engineered matrices allowed for extended release of pro-angiogenic factors and for their synergistic signaling with extracellular matrix-binding domains in the post-transplant period. Aprotinin addition delayed matrix degradation and prolonged pro-angiogenic factor availability within the graft. Both subcutaneous (SC) and epididymal fat pad (EFP) sites were evaluated. We show that in the SC site, diabetes reversal in mice transplanted with 1,000 IEQ of syngeneic islets was not observed for islets transplanted alone, while engineered matrices resulted in a diabetes median reversal time (MDRT) of 38 days. In the EFP site, the MDRT with 250 IEQ of syngeneic islets within the engineered matrices was 24 days versus 86 days for islets transplanted alone. Improved function of engineered grafts was associated with enhanced and earlier (by day 7) angiogenesis. Our findings show that by engineering the transplant site to promote prompt re-vascularization, engraftment and long-term function of islet grafts can be improved in relevant extrahepatic sites.


Biotechnol Bioeng


Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA




Has Abstract


2015-09-01 00:00:00












  • Evaluation of selectivity changes in HIC systems using a preferential interaction based analysis.

    abstract::It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xia F,Nagrath D,Garde S,Cramer SM

    更新日期:2004-08-05 00:00:00

  • Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains.

    abstract::A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

    更新日期:2002-12-30 00:00:00

  • Review: tissue engineering: reconstitution of human hematopoiesis ex vivo.

    abstract::The reconstruction of functioning human tissues ex vivo is becoming an important part of biotechnology. There are compelling scientific, clinical, and biotechnological reasons for fully or partially reconstituting human tissues such as skin, bone marrow, and liver ex vivo. In particular, bone marrow is a tissue of muc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Koller MR,Palsson BO

    更新日期:1993-10-01 00:00:00

  • Scale-Up of flat plate photobioreactors considering diffuse and direct light characteristics.

    abstract::This study investigates the scaling of photobioreactor productivity based on the growth of Nannochloropsis salina incorporating the effects of direct and diffuse light. The scaling and optimization of photobioreactor geometry was analyzed by determining the growth response of a small-scale system designed to represent...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Quinn JC,Turner CW,Bradley TH

    更新日期:2012-02-01 00:00:00

  • Adapting viral safety assurance strategies to continuous processing of biological products.

    abstract::There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Johnson SA,Brown MR,Lute SC,Brorson KA

    更新日期:2017-01-01 00:00:00

  • Dynamic modeling and optimal fed-batch feeding strategies for a two-phase partitioning bioreactor.

    abstract::A dynamic model for the degradation of phenol in a two-phase partitioning bioreactor has been developed based on mechanistic balances around the bioreactor. The key process characteristics including substrate transfer between the organic and aqueous phases, substrate inhibition, oxygen limitation, and cell entrainment...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cruickshank SM,Daugulis AJ,McLellan PJ

    更新日期:2000-01-20 00:00:00

  • Industrial symbiosis: corn ethanol fermentation, hydrothermal carbonization, and anaerobic digestion.

    abstract::The production of dry-grind corn ethanol results in the generation of intermediate products, thin and whole stillage, which require energy-intensive downstream processing for conversion into commercial animal feed products. Hydrothermal carbonization of thin and whole stillage coupled with anaerobic digestion was inve...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wood BM,Jader LR,Schendel FJ,Hahn NJ,Valentas KJ,McNamara PJ,Novak PM,Heilmann SM

    更新日期:2013-10-01 00:00:00

  • Calculation of entropy change accompanying growth of Escherichia coli K-12 on succinic acid.

    abstract::The DeltaSf of one unit carbon formula weight of Escherichia coli K-12 cells, when grown on succinic acid, was calculated to be -80.13 J/deg. This value could then be used to calculate the entropy change accompanying the anabolism and metabolism of succinic acid to be 30.82 J/deg and 32.40 J/mol deg, respectively. The...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Battley EH

    更新日期:1993-02-20 00:00:00

  • Modeling and analysis of layered stationary anaerobic granular biofilms.

    abstract::A model that portrays substrate profiles in a steady-state multispecies granular biofilm is developed and coupled with a biofilm detachment model. The model accounts for glucose, propionate, hydrogen, and acetate transformations performed by three bacterial trophic groups: acidogens, syntrophic bacterial consortia, an...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tartakovsky B,Guiot SR

    更新日期:1997-04-20 00:00:00

  • Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition.

    abstract::A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Goey CH,Tsang JMH,Bell D,Kontoravdi C

    更新日期:2017-12-01 00:00:00

  • Redesigning metabolic networks using mathematical programming

    abstract::The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dean JP,Dervakos GA

    更新日期:1998-04-05 00:00:00

  • Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar typhimurium.

    abstract::A recombinant strain of Salmonella enterica serovar Typhimurium (mutant in propionate-activation activity) was metabolically engineered to control the composition of poly(3-hydroxybutyrate-co-3-hydroxy- valerate) (PHBV), a polyhydroxyalkanoate copolymer with commercially desirable properties. A gene (prpE) encoding pr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Aldor I,Keasling JD

    更新日期:2001-09-01 00:00:00

  • A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis.

    abstract::The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang Z,Wang B,Hu Q,Sommerfeld M,Li Y,Han D

    更新日期:2016-10-01 00:00:00

  • Yield, solubility and conformational quality of soluble proteins are not simultaneously favored in recombinant Escherichia coli.

    abstract::Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational quality of recombinant ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Martínez-Alonso M,García-Fruitós E,Villaverde A

    更新日期:2008-12-15 00:00:00

  • The effect of medium composition on the acetone-butanol fermentation in continuous culture.

    abstract::The effects of growing Clostridium acetobutylicum NCIB 8052 in a chemostat under conditions of glucose, NH(4) (+), PO(4) (3-), Mg(2+), and Fe(2+) limitation were examined. It was noted that limitation of any major nutrient resulted in the same fermentation pattern. Conditions where minor nutrient levels were reduced a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: McNeil B,Kristiansen B

    更新日期:1987-02-01 00:00:00

  • Kinetic analysis of bioconversion of cellulose in attrition bioreactor.

    abstract::The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially incr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jones EO,Lee JM

    更新日期:1988-01-01 00:00:00

  • Differential stability of proteolytically active and inactive recombinant metalloproteinase in Chinese hamster ovary cells.

    abstract::Stability of heterologous protein expression during production is critical for regulatory approval of vaccine and therapeutic products. Leishmania GP63, a zinc metalloproteinase that is a potential vaccine candidate, has been expressed on the surface of Chinese hamster ovary (CHO) cells. Flow cytometry was used to fol...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Morrison CJ,McMaster WR,Piret JM

    更新日期:1997-03-20 00:00:00

  • Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Saccharomyces cerevisiae RWB222. Part II: investigation of discrepancies between predicted and observed performance at high solids concentration.

    abstract::The simultaneous saccharification and co-fermentation (SSCF) kinetic model described in the companion paper can predict batch and fed batch fermentations well at solids concentrations up to 62.4 g/L cellulose paper sludge but not in batch fermentation at 82.0 g/L cellulose paper sludge. Four hypotheses for the discrep...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang J,Shao X,Lynd LR

    更新日期:2009-12-01 00:00:00

  • Leveraging flow mechanics to determine critical process and scaling parameters in a continuous viral inactivation reactor.

    abstract::A continuous viral inactivation (CVI) chamber has been designed to operate with acceptable residence time distribution (RTD) characteristics. However, altering the CVI's geometry and operation to accommodate the scale was not obvious. In this work, we elucidate the influence of Dean vortices and leverage the transitio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brown MR,Orozco R,Coffman J

    更新日期:2020-03-01 00:00:00

  • Impact of external calcium and calcium sensors on ginsenoside Rb1 biosynthesis by Panax notoginseng cells.

    abstract::The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yue CJ,Zhong JJ

    更新日期:2005-02-20 00:00:00

  • Pilot trials of the microbial degradation of Christos-Bitas water in oil emulsion (chocolate mousse) and BP llandarcy gas oil using venturi aeration.

    abstract::Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and v...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Berwick PG

    更新日期:1985-01-01 00:00:00

  • Peptides derived from a secretory yeast library restore factor VIII activity in the presence of an inhibitory antibody.

    abstract::The development of autoantibodies against factor VIII represents one of the major complications in the treatment of hemophilia A patients. We have employed a novel library system to obtain peptides that specifically neutralize the interaction between factor VIII and these inhibitors. The random peptides are presented ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mersich C,Billes W,Pabinger I,Jungbauer A

    更新日期:2007-09-01 00:00:00

  • Metabolic engineering of Bacillus subtilis for production of D-lactic acid.

    abstract::Poly lactic acid (PLA) based plastics is renewable, bio-based, and biodegradable. Although present day PLA is composed of mainly L-LA, an L- and D- LA copolymer is expected to improve the quality of PLA and expand its use. To increase the number of thermotolerant microbial biocatalysts that produce D-LA, a derivative ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Awasthi D,Wang L,Rhee MS,Wang Q,Chauliac D,Ingram LO,Shanmugam KT

    更新日期:2018-02-01 00:00:00

  • Bacterial SCP from methanol in Kuwait: Product revovery and composition.

    abstract::The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Prokop A,Ratcliffe HD,Fatayer MI,Al-Awadhi N,Khamis A,Murad M,Bond C,Hamdan IY

    更新日期:1984-09-01 00:00:00

  • Quantitative and kinetic study of oxidative stress regulons using green fluorescent protein.

    abstract::Potentially damaging reactive oxygen species (ROS) are involved in a number of pathways ranging from signal transduction to apoptosis. Cells have adapted this alteration in redox status into a complex regulatory mechanism. ROS are specifically able to induce the expression of a multitude of genes. We constructed and c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lu C,Albano CR,Bentley WE,Rao G

    更新日期:2005-03-05 00:00:00

  • Extracellular hemoglobin combined with an O2 -generating material overcomes O2 limitation in the bioartificial pancreas.

    abstract::The bioartificial pancreas encapsulating pancreatic islets in immunoprotective hydrogel is a promising therapy for Type 1 diabetes. As pancreatic islets are highly metabolically active and exquisitely sensitive to hypoxia, maintaining O2 supply after transplantation remains a major challenge. In this study, we address...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mouré A,Bacou E,Bosch S,Jegou D,Salama A,Riochet D,Gauthier O,Blancho G,Soulillou JP,Poncelet D,Olmos E,Bach JM,Mosser M

    更新日期:2019-05-01 00:00:00

  • Dependence of biological treatment rate on species composition in activated sludge or biofilm. II: From models to theory.

    abstract::Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vavilin VA

    更新日期:1983-06-01 00:00:00

  • The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study.

    abstract::At the laboratory scale, sudden step increases from 30 to 42 degrees C can be readily accomplished when expressing heterologous proteins in heat-inducible systems. However, for large scale-cultures only slow ramp-type increases in temperature are possible due to heat transfer limitations, where the heating rate decrea...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Caspeta L,Flores N,Pérez NO,Bolívar F,Ramírez OT

    更新日期:2009-02-01 00:00:00

  • Metabolic engineering of acid resistance elements to improve acid resistance and propionic acid production of Propionibacterium jensenii.

    abstract::Propionic acid (PA) and its salts are widely used in the food, pharmaceutical, and chemical industries. Microbial production of PA by propionibacteria is a typical product-inhibited process, and acid resistance is crucial in the improvement of PA titers and productivity. We previously identified two key acid resistanc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Guan N,Li J,Shin HD,Du G,Chen J,Liu L

    更新日期:2016-06-01 00:00:00

  • Investigation of the TCA cycle and the glyoxylate shunt in Escherichia coli BL21 and JM109 using (13)C-NMR/MS.

    abstract::Acetate accumulation is a common problem observed in aerobic high cell density Escherichia coli cultures. A previous report has hypothesized that the glyoxylate shunt is active in a low acetate producer, E. coli BL21, and inactive in a high acetate producer, JM109. To further investigate this hypothesis, we now develo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Noronha SB,Yeh HJ,Spande TF,Shiloach J

    更新日期:2000-05-05 00:00:00