Correlating single cell motility with population growth dynamics for flagellated bacteria.

abstract：:Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

更新日期：2019-07-01 00:00:00

abstract：:Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...

journal_title：Biotechnology and bioengineering

authors： Ghosh P,Lin M,Vogel JH,Choy D,Haynes C,von Lieres E

更新日期：2014-08-01 00:00:00

abstract：:Isothermal microcalorimeters (IMC) are highly sensitive instruments that allow the measurement of heat flow in the microwatt range. Due to their detection of minute thermal heat, IMC techniques have been used in numerous biological applications, including the study of fermentation processes, pharmaceutical development...

journal_title：Biotechnology and bioengineering

authors： Santoro R,Braissant O,Müller B,Wirz D,Daniels AU,Martin I,Wendt D

更新日期：2011-12-01 00:00:00

abstract：:A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

journal_title：Biotechnology and bioengineering

authors： Wang M,Yu C,Zhao H

更新日期：2016-03-01 00:00:00

abstract：:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

journal_title：Biotechnology and bioengineering

authors： Moussy Y

更新日期：2000-04-20 00:00:00

abstract：:The bacterial consortium MPD-M, isolated from sediment associated with Colombian mangrove roots, was effective in the treatment of hydrocarbons in water with salinities varying from 0 to 180 g L(-1). Where the salinity of the culture medium surpassed 20 g L(-1), its effectiveness increased when the cells were immobili...

journal_title：Biotechnology and bioengineering

authors： Díaz MP,Boyd KG,Grigson SJ,Burgess JG

更新日期：2002-07-20 00:00:00

abstract：:The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size wit...

journal_title：Biotechnology and bioengineering

authors： Chan MY,Hoare M,Dunnill P

更新日期：1986-03-01 00:00:00

abstract：:Pronounced spatial nonuniformities in cell density, physiology, and activity frequently arise within densely packed immobilized cell supports. For a more fundamental understanding of immobilized cell phenomena, we have developed high-resolution microfluorimetric procedures to analyze local variations in both immobiliz...

journal_title：Biotechnology and bioengineering

authors： Kuhn RH,Peretti SW,Ollis DF

更新日期：1991-08-05 00:00:00

abstract：:Reduced downstream costs, together with high purity recovery of polyhydroxyalkanoate (PHA), will accelerate the commercialization of high quality PHA-based products. In this work, a process was designed for effective recovery of the copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) (P(HB-co-HHx)) containing high lev...

journal_title：Biotechnology and bioengineering

authors： Riedel SL,Brigham CJ,Budde CF,Bader J,Rha C,Stahl U,Sinskey AJ

更新日期：2013-02-01 00:00:00

abstract：:Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...

journal_title：Biotechnology and bioengineering

authors： Wayman FM,Mattey M

更新日期：2000-02-20 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:This paper presents an experimental analysis of matrix bead size distribution and voidage variations with axial height in an expanded bed adsorption system. Use of a specially constructed expanded bed with side ports has enabled sampling from within the expanded bed along the vertical axis. Particles removed from with...

journal_title：Biotechnology and bioengineering

authors： Willoughby NA,Hjorth R,Titchener-Hooker NJ

更新日期：2000-09-20 00:00:00

abstract：:An experimental device was constructed to allow nearly simultaneous measurements to be made on temperature and gas composition at different depths in a solid-substrate fermentation bed. The time-dependent values of temperature, mol % O(2) and mol % CO(2) were measured at five positions in beds 6.35 cm (2.5 in.) deep. ...

journal_title：Biotechnology and bioengineering

authors： Rathbun BL,Shuler ML

更新日期：1983-04-01 00:00:00

abstract：:In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...

journal_title：Biotechnology and bioengineering

authors： Ottengraf SP,Van Den Oever AH

更新日期：1983-12-01 00:00:00

abstract：:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...

journal_title：Biotechnology and bioengineering

authors： Park JB,Clark DS

更新日期：2006-05-05 00:00:00

abstract：:Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale-up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model ...

journal_title：Biotechnology and bioengineering

authors： Souret FF,Kim Y,Wyslouzil BE,Wobbe KK,Weathers PJ

更新日期：2003-09-20 00:00:00

abstract：:Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and t...

journal_title：Biotechnology and bioengineering

authors： Bok M,Li H,Yeo LY,Friend JR

更新日期：2009-06-01 00:00:00

abstract：:The performance and microbial communities of three differently inoculated biotrickling filters removing dimethyl sulfide (DMS) were compared. The biotrickling filters were inoculated with Thiobacillus thioparus TK-m (THIO), sludge (HANDS) and sludge + T. thioparus TK-m + Hyphomicrobium VS (HANDS++), respectively. The ...

journal_title：Biotechnology and bioengineering

authors： Sercu B,Boon N,Beken SV,Verstraete W,Van Langenhove H

更新日期：2007-03-01 00:00:00

abstract：:A number of bioreactor configurations have been developed for the manufacture of products from mammalian cell hosts. Even in the most efficient of these, however, problems such as nutrient exhaustion, growth factor deprivation, and toxin accumulations may arise. Consequently, the current effort focused on the feasibil...

journal_title：Biotechnology and bioengineering

authors： Mastrangelo AJ,Hardwick JM,Zou S,Betenbaugh MJ

更新日期：2000-03-05 00:00:00

abstract：:Application of the well-characterized Streptococcus pyogenes CRISPR-Cas9 system in actinomycetes streptomycetes has enabled high-efficiency multiplex genome editing and CRISPRi-mediated transcriptional regulation in these prolific bioactive metabolite producers. Nonetheless, SpCas9 has its limitations and can be ineff...

journal_title：Biotechnology and bioengineering

authors： Yeo WL,Heng E,Tan LL,Lim YW,Lim YH,Hoon S,Zhao H,Zhang MM,Wong FT

更新日期：2019-09-01 00:00:00

abstract：:Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without the addition of plasmids or foreign genes. This was done by a combination of gene deletions (genetic engineering) and metabolic ...

journal_title：Biotechnology and bioengineering

authors： Jantama K,Haupt MJ,Svoronos SA,Zhang X,Moore JC,Shanmugam KT,Ingram LO

更新日期：2008-04-01 00:00:00

abstract：:Modern large-scale agricultural practices that incorporate high density farming with subtherapeutic antibiotic dosing are considered a major contributor to the rise of antibiotic-resistant bacterial infections of humans with species of Salmonella being a leading agriculture-based bacterial infection. Microcin J25, a p...

journal_title：Biotechnology and bioengineering

authors： Ritter SC,Yang ML,Kaznessis YN,Hackel BJ

更新日期：2018-10-01 00:00:00

abstract：:Perfusion bioreactors have been used in different tissue engineering applications because of their consistent distribution of nutrients and flow-induced shear stress within the tissue-engineering scaffold. A widely used configuration uses a scaffold with a circular cross-section enclosed within a cylindrical chamber a...

journal_title：Biotechnology and bioengineering

authors： Hidalgo-Bastida LA,Thirunavukkarasu S,Griffiths S,Cartmell SH,Naire S

更新日期：2012-04-01 00:00:00

abstract：:A propanologenic (i.e., 1-propanol-producing) bacterium Escherichia coli strain was previously derived by activating the genomic sleeping beauty mutase (Sbm) operon. The activated Sbm pathway branches out of the tricarboxylic acid (TCA) cycle at the succinyl-CoA node to form propionyl-CoA and its derived metabolites o...

journal_title：Biotechnology and bioengineering

authors： Miscevic D,Mao JY,Moo-Young M,Chou CP

更新日期：2020-05-01 00:00:00

abstract：:The enzymatic synthesis of polyesters from activated diesters and diols has been investigated. Differences between enzymatic synthesis and traditional chemical condensation processes are discussed. The disappearance of monomers during the initial phase of reaction indicates that enzyme has a higher specificity for tra...

journal_title：Biotechnology and bioengineering

authors： Chaudhary AK,Beckman EJ,Russell AJ

更新日期：1997-07-05 00:00:00

abstract：:Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the nativ...

journal_title：Biotechnology and bioengineering

authors： Doran JB,Aldrich HC,Ingram LO

更新日期：1994-06-20 00:00:00

abstract：:Acid phosphatase thermal deactivation follows a complex path: an initial decay toward an equilibrium distribution of at least two intermediate structures, mutually at the equilibrium, followed by a final breakdown toward a completely inactive enzyme configuration. The results obtained in the presence of sorbitol have ...

journal_title：Biotechnology and bioengineering

authors： Gianfreda L,Toscano G,Pirozzi D,Greco G Jr

更新日期：1991-12-05 00:00:00

abstract：:In this short review, we describe the use of high molecular weight proteins produced in the glands of several arthropods-commonly called silks-for the purpose to enhance human skin wound healing. To this end an extensive literature search has been performed, the publications have been categorized concerning silk prepa...

journal_title：Biotechnology and bioengineering

authors： Reimers K,Liebsch C,Radtke C,Kuhbier JW,Vogt PM

更新日期：2015-11-01 00:00:00

abstract：:Tissue function is comprised of a complex interplay between biological and physicochemical rate processes. The design of bioreactors for tissue engineering must account for these processes simultaneously in order to obtain a bioreactor that provides a uniform environment for tissue growth and development. In the prese...

journal_title：Biotechnology and bioengineering

authors： Peng CA,Palsson BØ

更新日期：1996-06-05 00:00:00

abstract：:In this study we describe a model that estimates the extracellular (nonfungal) and overall water contents of wheat grains during solid-state fermentation (SSF) with Aspergillus oryzae, using on-line measurements of oxygen, carbon dioxide, and water vapor in the gas phase. The model uses elemental balances to predict s...

journal_title：Biotechnology and bioengineering

authors： Nagel FJ,Tramper J,Bakker MS,Rinzema A

更新日期：2001-01-20 00:00:00