Efficient production of poly γ-d-glutamic acid from the bloom-forming green macroalgae, Ulva sp., by Bacillus sp. SJ-10.

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this stud...

journal_title：Biotechnology and bioengineering

authors： Baek K,Yu J,Jeong J,Sim SJ,Bae S,Jin E

更新日期：2018-03-01 00:00:00

abstract：:Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Marletta MA,Klibanov AM

更新日期：1987-07-01 00:00:00

abstract：:A model is developed to predict batch and continuous culture behavior of fermentations on two different carbon and energy sources. The basic assumption of the model is that the permease for the favored substrate is constitutive, whereas the premease for the second substrate is subject to induction and catabolite repre...

journal_title：Biotechnology and bioengineering

authors： Van Dedem G,Moo-Young M

更新日期：1975-09-01 00:00:00

abstract：:A structured kinetic model describing growth, differentiation, and penicillin production in submerged Penicillium chrysogenum fermentations is reported. The filamentous hyphae are divided into four distinct regions on the basis of the activities and structure of hyphal compartments, viz., actively growing (mainly apic...

journal_title：Biotechnology and bioengineering

authors： Paul GC,Thomas CR

更新日期：1996-09-05 00:00:00

abstract：:We designed a bioartificial liver support system in which encapsulated multicellular spheroids of rat hepatocytes were utilized as a bioreactor in a hollow fiber cartridge. The spheroids, formed in a positively charged polystyrene dish that contained hormonally defined medium, were encapsulated into microdroplets of a...

journal_title：Biotechnology and bioengineering

authors： Shiraha H,Koide N,Hada H,Ujike K,Nakamura M,Shinji T,Gotoh S,Tsuji T

更新日期：1996-05-20 00:00:00

abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...

journal_title：Biotechnology and bioengineering

authors： Li SW,Yu B,Byrne G,Wright M,O'Rourke S,Mesa K,Berman PW

更新日期：2019-09-01 00:00:00

abstract：:The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The value...

journal_title：Biotechnology and bioengineering

authors： Sokol W

更新日期：1987-12-05 00:00:00

abstract：:Yeast alcohol dehydrogenase (ADH) solutions (approximately 1 mg/ml, pH 7) were sheared in a coaxial cylindrical viscometer. This was fitted with a lid sealing the contents from the atmosphere and preventing evaporation. At 30 degrees C after a total of 5 hr intermittent shearing at 683 sec-1 no losses of activity were...

journal_title：Biotechnology and bioengineering

authors： Thomas CR,Nienow AW,Dunnill P

更新日期：1979-12-01 00:00:00

abstract：:Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos is characterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, ...

journal_title：Biotechnology and bioengineering

authors： Chi CM,Zhang C,Staba EJ,Cooke TJ,Hu WS

更新日期：1996-04-05 00:00:00

abstract：:In order to evaluate the sensitivity of hepatocellular cultures to variations in both substrate stiffness and bioactive ligand presentation, hepatocytes were cultured on differentially compliant polyacrylamide gel discs functionalized with varying amounts of the ECM ligand, fibronectin (FN). Subconfluent cell cultures...

journal_title：Biotechnology and bioengineering

authors： Semler EJ,Lancin PA,Dasgupta A,Moghe PV

更新日期：2005-02-05 00:00:00

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:Mathematical models to describe transcription (Arnold et al. (2001); Biotech Bioeng 72:548-561) and translation (Mehra et al. (2003); Biotech Bioeng 84:822-841) in bacteria are modified in order to improve reaction kinetics and to include the number of polymerase molecules that are active on the DNA, as well as to inc...

journal_title：Biotechnology and bioengineering

authors： Kremling A

更新日期：2007-03-01 00:00:00

abstract：:This research investigates the effect of solids retention time (SRT) on the acid-phase anaerobic digestion of primary sludge. A series of experiments were conducted using two continuous-flow 3-L units with the following configuration: a completely mixed reactor (CMR) with clarifier and solids recycle and an upflow ana...

journal_title：Biotechnology and bioengineering

authors： Elefsiniotis P,Oldham WK

更新日期：1994-06-05 00:00:00

abstract：:Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separate...

journal_title：Biotechnology and bioengineering

authors： Adachi M,Yamazaki M,Harada M,Shioi A,Katch S

更新日期：1997-02-20 00:00:00

abstract：:Low-temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a ...

journal_title：Biotechnology and bioengineering

authors： Kawakami M,Koya H,Gondo S

更新日期：1988-07-20 00:00:00

abstract：:A stirred bath technique was developed for determining effective diffusivities in cell matrices. The technique involves cell immobilization in a dilute gel which has negligible effect on solute diffusion. Agar and collagen were tested as immobilizing gels. Agar gel was shown to have minor interactions with the diffusi...

journal_title：Biotechnology and bioengineering

authors： Chresand TJ,Dale BE,Hanson SL,Gillies RJ

更新日期：1988-10-05 00:00:00

abstract：:Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...

journal_title：Biotechnology and bioengineering

authors： Kamata K,Toya Y,Shimizu H

更新日期：2019-05-01 00:00:00

abstract：:Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...

journal_title：Biotechnology and bioengineering

authors： Cheng SY,Constantinidis I,Sambanis A

更新日期：2006-04-20 00:00:00

abstract：:The development of renewable alternatives to diesel and jet fuels is highly desirable for the heavy transportation sector, and would offer benefits over the production and use of short-chain alcohols for personal transportation. Here, we report the development of a metabolically engineered strain of Escherichia coli t...

journal_title：Biotechnology and bioengineering

authors： Lennen RM,Braden DJ,West RA,Dumesic JA,Pfleger BF

更新日期：2010-06-01 00:00:00

abstract：:Using our recently developed sensor reactor approach, lysine-producing, nongrowing Corynebacterium glutamicum MH20-22B cells were subjected to serial (13)C-labeling experiments for flux analysis during the leucine-limited fed-batch production phase in a 300-L bioreactor. Based on two-dimensional (2D) nuclear magnetic ...

journal_title：Biotechnology and bioengineering

authors： Drysch A,El Massaoudi M,Wiechert W,de Graaf AA,Takors R

更新日期：2004-03-05 00:00:00

abstract：:In our previous study [Hong Y, Brown DG (2009) Appl Environ Microbiol 75(8):2346-2353], the adenosine triphosphate (ATP) level of adhered bacteria was observed to be 2-5 times higher than that of planktonic bacteria. Consequently, the proton motive force (Delta p) of adhered bacteria was approximately 15% greater than...

journal_title：Biotechnology and bioengineering

authors： Hong Y,Brown DG

更新日期：2010-04-01 00:00:00

abstract：:Various structure-similar plant secondary metabolites like ginseng saponins (ginsenosides) possess different or even totally opposite biological activities. Intentional manipulation of the ginsenoside heterogeneity in cellular biosynthesis is of great interest and significance [Zhong and Yue (2005); Adv Biochem Eng Bi...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhou X,Zhong JJ

更新日期：2008-08-01 00:00:00

abstract：:The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations...

journal_title：Biotechnology and bioengineering

authors： Pih N,De Bernardez E,Dhurjati P

更新日期：1988-03-01 00:00:00

abstract：:The mean sizes and size distributions of air bubbles and viscous castor oil drops were studied in a salt-rich aqueous solution (medium), first separately, and then simultaneously as a three-phase system. The dispersion was created in a 150-mm-diameter stirred tank equipped with a Rushton turbine, and the sizes were me...

journal_title：Biotechnology and bioengineering

authors： Galindo E,Pacek AW,Nienow AW

更新日期：2000-07-20 00:00:00

abstract：:We studied the resolution of racemic glycidol through esterification with butyric acid catalyzed by porcine pancreatic lipase in organic media. A screening of seven solvents (log P values between 0.49 and 3.0, P being the n-octanol-water partition coefficient of the solvent) showed that neither log P nor the logarithm...

journal_title：Biotechnology and bioengineering

authors： Martins JF,Da Ponte MN,Barreiros S

更新日期：1993-08-05 00:00:00

abstract：:A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs-Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox-magnetohydrodynamics (redox-MHD) conditions. Redox-MHD possesses uni...

journal_title：Biotechnology and bioengineering

authors： Cheah LT,Fritsch I,Haswell SJ,Greenman J

更新日期：2012-07-01 00:00:00

abstract：:Pulses of several nonreacting solutes were fed to a column containing immobilized enzyme catalyst beads. The response curves were recorded, and their first and second moments were determined by numerical integration. From the moment analysis, the void volume, internal porosity, and tortuosity of the support were calcu...

journal_title：Biotechnology and bioengineering

authors： Sirotti DA,Emery A

更新日期：1983-07-01 00:00:00

abstract：:N-linked glycan distribution affects important end-use characteristics such as the bioactivity and efficacy of many therapeutic proteins, (including monoclonal antibodies), in vivo. Yet, obtaining desired glycan distributions consistently during batch-to-batch production can be challenging for biopharmaceutical manufa...

journal_title：Biotechnology and bioengineering

authors： St Amand MM,Radhakrishnan D,Robinson AS,Ogunnaike BA

更新日期：2014-10-01 00:00:00

abstract：:Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...

journal_title：Biotechnology and bioengineering

authors： Shimizu K,Kobayashi T,Nagara A,Matsubara M

更新日期：1985-05-01 00:00:00