High-level heterologous production of propionate in engineered Escherichia coli.

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:A dynamic model for the degradation of phenol in a two-phase partitioning bioreactor has been developed based on mechanistic balances around the bioreactor. The key process characteristics including substrate transfer between the organic and aqueous phases, substrate inhibition, oxygen limitation, and cell entrainment...

journal_title：Biotechnology and bioengineering

authors： Cruickshank SM,Daugulis AJ,McLellan PJ

更新日期：2000-01-20 00:00:00

abstract：:Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

journal_title：Biotechnology and bioengineering

authors： Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

更新日期：2006-02-05 00:00:00

abstract：:A two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. The first bioreactor was employed for cell growth and the second for cell infection. Silkworm Bm5 cells were infected with a recombinant baculovirus, BmNPV/P...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Kalogerakis N,Behie LA,Iatrou K

更新日期：1993-07-01 00:00:00

abstract：:A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...

journal_title：Biotechnology and bioengineering

authors： Agena SM,Pusey ML,Bogle ID

更新日期：1999-07-20 00:00:00

abstract：:Alginate gels produced by an external or internal gelation technique were studied so as to determine the optimal bead matrix within which DNA can be immobilized for in vivo application. Alginates were characterized for guluronic/mannuronic acid (G/M) content and average molecular weight using 1H-NMR and LALLS analysis...

journal_title：Biotechnology and bioengineering

authors： Quong D,Neufeld RJ,Skjåk-Braek G,Poncelet D

更新日期：1998-02-20 00:00:00

abstract：:Growth of microorganisms on substitutable substrate mixtures display diverse growth dynamics characterized by simultaneous or preferential uptake of carbon sources. This article shows that cybernetic modeling concepts which were successful in predicting diauxic growth patterns can be extended to describe simultaneous ...

journal_title：Biotechnology and bioengineering

authors： Ramakrishna R,Ramkrishna D,Konopka AE

更新日期：1996-10-05 00:00:00

abstract：:The cellulase system of Bacteroides cellulosolvens was subjected to both catabolite repression and feedback inhibition by cellobiose. Cellulose-solubilizing activity was 50% inhibited at a cellobiose concentration of 2.6 g/L and completely inhibited by 12 g/L. Glucose at 12 g/L (the highest concentration tested) had n...

journal_title：Biotechnology and bioengineering

authors： Murray WD

更新日期：1987-06-01 00:00:00

abstract：:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined i...

journal_title：Biotechnology and bioengineering

authors： Kimura M,Michizoe J,Oakazaki SY,Furusaki S,Goto M,Tanaka H,Wariishi H

更新日期：2004-11-20 00:00:00

abstract：:Centrifugal adsorption technology (CAT) is a new compact, countercurrent technology for efficient adsorption from large liquid streams by using adsorbent particles in the micrometer range. CAT seems particularly suited for the recovery of macromolecules at low concentrations, because the small particle dimensions lead...

journal_title：Biotechnology and bioengineering

authors： Peet DJ,Bisschops MA,van Hateren SH,van der Wielen LA

更新日期：2002-05-05 00:00:00

abstract：:Autoflocculation and bioflocculation are considered to be the most promising means for the economical harvesting of microalgae. We have therefore studied these phenomena with cultures of Scenedesmus obliquus produced during biological tertiary wastewater treatment. The quantity of extracellular polymers produced durin...

journal_title：Biotechnology and bioengineering

authors： Lavoie A,de la Noüe J

更新日期：1987-11-01 00:00:00

abstract：:A novel method has been developed for the separation of bioproducts from yeast cells. The method uses a combination of physical, chemical, and biological agents such as lytic enzymes, osmotic supports, and spheroplast stabilizers. Using this technique, products (proteins and enzymes) can be released from specific cell...

journal_title：Biotechnology and bioengineering

authors： Huang RB,Andrews BA,Asenjo JA

更新日期：1991-11-01 00:00:00

abstract：:In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulations were performed to manipulate microparticles toward target areas. Base...

journal_title：Biotechnology and bioengineering

authors： Ino K,Shiku H,Ozawa F,Yasukawa T,Matsue T

更新日期：2009-11-01 00:00:00

abstract：:In this study, white rot fungus, Polyporus brumalis, was applied to degrade dibutyl phthalate (DBP), a major environmental pollutant. The degradation potential and resulting products were evaluated with HPLC and GC/MS. As DBP concentration increased to 250, 750, and 1,250 microM, the mycelial growth of P. brumalis was...

journal_title：Biotechnology and bioengineering

authors： Lee SM,Lee JW,Koo BW,Kim MK,Choi DH,Choi IG

更新日期：2007-08-15 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...

journal_title：Biotechnology and bioengineering

authors： Saucedo VM,Karim MN

更新日期：1997-07-20 00:00:00

abstract：:In vitro engineering systems can be powerful tools for studying tissue development in response to biophysical stimuli as well as for evaluating the functionality of engineered tissue grafts. It has been challenging, however, to develop systems that adequately integrate the application of biomimetic mechanical strain t...

journal_title：Biotechnology and bioengineering

authors： Cook CA,Huri PY,Ginn BP,Gilbert-Honick J,Somers SM,Temple JP,Mao HQ,Grayson WL

更新日期：2016-08-01 00:00:00

abstract：:Clostridium tyrobutyricum ATCC 25755 is known as a natural hyper-butyrate producer with great potentials as an excellent platform to be engineered for valuable biochemical production from renewable resources. However, limited transformation efficiency and the lack of genetic manipulation tools have hampered the broade...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Hong W,Guo L,Wang Y,Wang Y

更新日期：2020-09-01 00:00:00

abstract：:Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

journal_title：Biotechnology and bioengineering

authors： Siano SA

更新日期：1996-12-20 00:00:00

abstract：:Although microalgae are considered as a promising feedstock for biofuels, the energy efficiency of the production process needs to be significantly improved. Due to their small size and low concentration in the culture medium, cost-efficient harvesting of microalgae is a major challenge. In this study, the use of elec...

journal_title：Biotechnology and bioengineering

authors： Vandamme D,Pontes SC,Goiris K,Foubert I,Pinoy LJ,Muylaert K

更新日期：2011-10-01 00:00:00

abstract：:Cellulase was modified with amphilic copolymers made of alpha-allyl-omega-methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer....

journal_title：Biotechnology and bioengineering

authors： Park JW,Kajiuchi T

更新日期：1995-02-20 00:00:00

abstract：:Extractive work-up of whole-cell biotransformations generally suffers from the formation of stable gels and slimes upon addition of the organic solvent to the cell suspension and the cell-free solution, respectively. This problem has been overcome by enzymatic lysis of emulsifying agents present in the medium through ...

journal_title：Biotechnology and bioengineering

authors： Jörg G,Leppchen K,Daussmann T,Bertau M

更新日期：2004-08-20 00:00:00

abstract：:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

journal_title：Biotechnology and bioengineering

authors： Schinkel H,Jacobs P,Schillberg S,Wehner M

更新日期：2008-01-01 00:00:00

abstract：:Accurate measurement of enzymatic cellulose digestibility (X) is important in evaluating the efficiency of lignocellulose pretreatment technologies, assessing the performance of reconstituted cellulase mixtures, and conducting economic analysis for biorefinery processes. We analyzed the effect of sugars contained in e...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Schell DJ,McMillan JD

更新日期：2007-01-01 00:00:00

abstract：:Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (GFP) and a monoclonal antibody as reporter proteins. Following t...

journal_title：Biotechnology and bioengineering

authors： Chenuet S,Martinet D,Besuchet-Schmutz N,Wicht M,Jaccard N,Bon AC,Derouazi M,Hacker DL,Beckmann JS,Wurm FM

更新日期：2008-12-01 00:00:00

abstract：:A microarray targeting four sequenced strains in the Dehalococcoides (Dhc) genus was used to analyze gene expression in a robust long-term trichloroethene (TCE)-degrading microbial community (designated ANAS) during feeding cycles that involve conditions of periodic substrate supply. The Dhc transcriptome was examined...

journal_title：Biotechnology and bioengineering

authors： West KA,Lee PK,Johnson DR,Zinder SH,Alvarez-Cohen L

更新日期：2013-05-01 00:00:00

abstract：:Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. H...

journal_title：Biotechnology and bioengineering

authors： Kamiya K,Tsumoto K,Arakawa S,Shimizu S,Morita I,Yoshimura T,Akiyoshi K

更新日期：2010-12-01 00:00:00

abstract：:Foam separation may have potential for protein recovery. However, for foam separation to be a viable protein recovery technique it is important to demonstrate, not only that high enrichments and recoveries can be achieved for single proteins, but also that high enrichments and recoveries, together with selectivity of ...

journal_title：Biotechnology and bioengineering

authors： Brown AK,Kaul A,Varley J

更新日期：1999-02-05 00:00:00

abstract：:The accumulation of inorganic phosphate inhibits protein synthesis in cell-free protein synthesis reactions that are energized by high-energy-phosphate-containing compounds. This study developed a new scheme for supplying energy using dual energy sources to enhance the regeneration of ATP and lower the rate of phospha...

journal_title：Biotechnology and bioengineering

authors： Kim TW,Oh IS,Keum JW,Kwon YC,Byun JY,Lee KH,Choi CY,Kim DM

更新日期：2007-08-15 00:00:00

abstract：:Increasing the throughput and efficiency of cell culture process development has become increasingly important to rapidly screen and optimize cell culture media and process parameters. This study describes the application of a miniaturized bioreactor system as a scaled-down model for cell culture process development u...

journal_title：Biotechnology and bioengineering

authors： Chen A,Chitta R,Chang D,Amanullah A

更新日期：2009-01-01 00:00:00