Preparation of connexin43-integrated giant Liposomes by a baculovirus expression-liposome fusion method.

abstract：:Steady-state simulations using our previously developed structured kinetic model of antibody synthesis and secretion by hybridoma cells are used here in conjunction with factorial design analysis to identify intracellular parameters important in determining the specific antibody secretion rate and predict the dependen...

journal_title：Biotechnology and bioengineering

authors： Bibila TA,Flickinger MC

更新日期：1992-02-05 00:00:00

abstract：:Affinity chromatography using immobilized S-protein was used for the screening of affinity peptide ligands from two soluble peptide libraries. Peptide library I consisted of octamers with glycine (G) at both termini of each peptide, i.e. GXXXXXXG. The six center positions were constructed using random sequences of six...

journal_title：Biotechnology and bioengineering

authors： Huang PY,Carbonell RG

更新日期：1999-06-20 00:00:00

abstract：:In this study we report the isolation of four denitrifying bacteria from a batch reactor, where the progress of hydrogenotrophic denitrification was examined. Only three of the strains had the ability to use hydrogen as electron donor. In the present work, kinetic batch experiments were carried out in order to study t...

journal_title：Biotechnology and bioengineering

authors： Vasiliadou IA,Siozios S,Papadas IT,Bourtzis K,Pavlou S,Vayenas DV

更新日期：2006-10-20 00:00:00

abstract：:Pretreatment of high-crystalline cellulose with N-methyl-morpholine-N-oxide (NMO or NMMO) to improve bioethanol and biogas production was investigated. The pretreatments were performed at 90 and 120 degrees C for 0.5-15 h in three different modes, including dissolution (85% NMO), ballooning (79% NMO), and swelling (73...

journal_title：Biotechnology and bioengineering

authors： Jeihanipour A,Karimi K,Taherzadeh MJ

更新日期：2010-02-15 00:00:00

abstract：:This article studies the operation of a new process for the production of biopolymers (polyhydroxyalkanoates, PHAs) at different applied organic load rates (OLRs). The process is based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store PHAs at high rates and yields. A mixture of aceti...

journal_title：Biotechnology and bioengineering

authors： Dionisi D,Majone M,Vallini G,Di Gregorio S,Beccari M

更新日期：2006-01-05 00:00:00

abstract：:Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without sel...

journal_title：Biotechnology and bioengineering

authors： González Vara A,Rossi M,Altomare L,Eikmanns B,Matteuzzi D

更新日期：2003-10-20 00:00:00

abstract：:The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...

journal_title：Biotechnology and bioengineering

authors： Bielefeldt AR,Cort T

更新日期：2005-03-20 00:00:00

abstract：:A nuclear magnetic resonance (NMR) method has been developed to monitor on-line lipase-catalyzed esterification reactions without the need to sample the reaction medium. The technique, through (1)H NMR, measures the concentrations of alcohol, ester, hydroxylic hydrogens in the organic phase, and hydroxylic hydrogens i...

journal_title：Biotechnology and bioengineering

authors： Sarazin C,Ergan F,Séguin JP,Goethals G,Legoy MD,Barbotin JN

更新日期：1996-09-20 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:In this study, omics-based analysis tools were used to explore the effect of glucose starvation and culture duration on monoclonal antibody (mAb) production in fed-batch CHO cell culture to gain better insight into how these parameters can be controlled to ensure optimal mAb productivity and quality. Titer and N-glyco...

journal_title：Biotechnology and bioengineering

authors： Fan Y,Jimenez Del Val I,Müller C,Lund AM,Sen JW,Rasmussen SK,Kontoravdi C,Baycin-Hizal D,Betenbaugh MJ,Weilguny D,Andersen MR

更新日期：2015-10-01 00:00:00

abstract：:Isothermal microcalorimeters (IMC) are highly sensitive instruments that allow the measurement of heat flow in the microwatt range. Due to their detection of minute thermal heat, IMC techniques have been used in numerous biological applications, including the study of fermentation processes, pharmaceutical development...

journal_title：Biotechnology and bioengineering

authors： Santoro R,Braissant O,Müller B,Wirz D,Daniels AU,Martin I,Wendt D

更新日期：2011-12-01 00:00:00

abstract：:Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

journal_title：Biotechnology and bioengineering

authors： Braud A,Jézéquel K,Léger MA,Lebeau T

更新日期：2006-08-20 00:00:00

abstract：:Among the host of substratum properties that affect animal cell behavior, surface morphology has received relatively little attention. The earliest effect of surface morphology on animal cells was discovered almost a century ago when it was found that cells became oriented in response to the underlying topography. Thi...

journal_title：Biotechnology and bioengineering

authors： Singhvi R,Stephanopoulos G,Wang DI

更新日期：1994-04-05 00:00:00

abstract：:We developed a self-assembling polymer based on polyallylamine (PAH) for use in DNA chips. Thioctic acid (TA) was covalently attached to PAH in sidechains to immobilize the polymer on a gold surface by self-assembly. N-hydroxysuccinimide-ester terminated probe single-stranded (ss) DNA is easily covalently immobilized ...

journal_title：Biotechnology and bioengineering

authors： Taira S,Yokoyama K

更新日期：2005-03-30 00:00:00

abstract：:Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...

journal_title：Biotechnology and bioengineering

authors： Docoslis A,Kalogerakis N,Behie LA,Kaler KV

更新日期：1997-05-05 00:00:00

abstract：:Trypsin and alpha-chymotrypsin were immobilized by gelentrapment in polyacrylamide cross-linked with N,N(1)-methylenebisacrylamide. The immobilized enzymes are catalytically efficient in suspensions of reverse micelles formed in isooctane by bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and water. Both entrapped enzym...

journal_title：Biotechnology and bioengineering

authors： Fadnavis NW,Luisi PL

更新日期：1989-04-20 00:00:00

abstract：:The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass without allowing glucose to be diverted into the reductive meta...

journal_title：Biotechnology and bioengineering

authors： Duboc P,Cascão-Pereira LG,von Stockar U

更新日期：1998-03-05 00:00:00

abstract：:Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen ...

journal_title：Biotechnology and bioengineering

authors： Nandi R,Dey S,Sengupta S

更新日期：2001-11-20 00:00:00

abstract：:Mathematical modeling of animal cell growth and metabolism is essential for the understanding and improvement of the production of biopharmaceuticals. Models can explain the dynamic behavior of cell growth and product formation, support the identification of the most relevant parameters for process design, and signifi...

journal_title：Biotechnology and bioengineering

authors： Ramos JRC,Rath AG,Genzel Y,Sandig V,Reichl U

更新日期：2020-05-01 00:00:00

abstract：:Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separate...

journal_title：Biotechnology and bioengineering

authors： Adachi M,Yamazaki M,Harada M,Shioi A,Katch S

更新日期：1997-02-20 00:00:00

abstract：:Clostridium carboxidivorans P7 is one of three microbial catalysts capable of fermenting synthesis gas (mainly CO, CO(2) , and H(2) ) to produce the liquid biofuels ethanol and butanol. Gasification of feedstocks to produce synthesis gas (syngas), followed by microbial conversion to solvents, greatly expands the diver...

journal_title：Biotechnology and bioengineering

authors： Ukpong MN,Atiyeh HK,De Lorme MJ,Liu K,Zhu X,Tanner RS,Wilkins MR,Stevenson BS

更新日期：2012-11-01 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...

journal_title：Biotechnology and bioengineering

authors： James RI,Elton JP,Todd P,Kompala DS

更新日期：2000-01-20 00:00:00

abstract：:Steam explosion after sulphur dioxide impregnation of wood chips is an effective method for improving the enzymatic digestibility of cellulose in the softwood Pinus radiata. Digestibility of pretreated fiber was progressively increased by altering the conditions of steam explosion. With increasing digestibility, there...

journal_title：Biotechnology and bioengineering

authors： Wong KK,Deverell KF,Mackie KL,Clark TA,Donaldson LA

更新日期：1988-04-05 00:00:00

abstract：:Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...

journal_title：Biotechnology and bioengineering

authors： Selvarasu S,Ow DS,Lee SY,Lee MM,Oh SK,Karimi IA,Lee DY

更新日期：2009-02-15 00:00:00

abstract：:Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-...

journal_title：Biotechnology and bioengineering

authors： Tang Q,Piard C,Lin J,Nan K,Guo T,Caccamese J,Fisher J,Chen Y

更新日期：2018-01-01 00:00:00

abstract：:The starting point for the present investigations was the finding that increasing influent concentrations from 10 to 380 mmol/L glucose decreased the attainable growth rate of an acidogenic population in continuous culture from 0.52 to 0.05 h(-1) To account for this phenomenon, a new kinetic model is developed that co...

journal_title：Biotechnology and bioengineering

authors： Van Den Heuvel JC,Beeftink HH

更新日期：1988-05-01 00:00:00

abstract：:The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is ...

journal_title：Biotechnology and bioengineering

authors： Yousefpour P,Chilkoti A

更新日期：2014-09-01 00:00:00

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...

journal_title：Biotechnology and bioengineering

authors： Soriano E,Borth N,Katinger H,Mattanovich D

更新日期：2002-10-05 00:00:00